LOCUS       40924_21075        7942 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Agrobacterium binary gap repair vector pGAP-Hyg, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7942)
  AUTHORS   Bitrian M, Roodbarkelari F, Horvath M, Koncz C.
  TITLE     BAC-recombineering for studying plant gene regulation: developmental
            control and cellular localization of SnRK1 kinase subunits
  JOURNAL   Plant J. 65 (5), 829-842 (2011)
  PUBMED    21235649
REFERENCE   2  (bases 1 to 7942)
  AUTHORS   Bitrian M, Koncz C.
  TITLE     Agrobacterium binary gap repair vectors for recombineering with 
            Arabidopsis BACs
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 7942)
  AUTHORS   Bitrian M, Koncz C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JUL-2008) Plant Developmental Biology, Max-Planck 
            Institute for Plant Breeding Research, Carl-von-Linne-Weg 10, 
            Cologne, NRW 50829, Germany
REFERENCE   4  (bases 1 to 7942)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 7942)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J."; 
            date: "2011"; volume: "65"; issue: "5"; pages: "829-842"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (30-JUL-2008) Plant Developmental Biology, Max-Planck Institute for 
            Plant Breeding Research, Carl-von-Linne-Weg 10, Cologne, NRW 50829, 
            Germany"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7942
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..59
                     /note="polylinker: EcoRI, HindIII, HindIII, XbaI, BglII,
                     PstI, SalI, XbaI, BamHI"
     misc_feature    64..383
                     /note="derived from Escherichia coli transposon Tn5
                     sequence in GenBank Accession Number U00004"
     promoter        467..646
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             691..1713
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
     terminator      1747..1999
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    2059..2083
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     oriT            complement(3211..3320)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      4114..4825
                     /label=oriV
                     /note="incP origin of replication"
     misc_feature    4829..5124
                     /note="derived from Birmingham IncP-alpha plasmid RK2 
                     (gb|L27758.1|BIACOMGEN; NC_001621)"
     misc_feature    5412..5436
                     /label=LB T-DNA repeat
                     /note="left border repeat from octopine Ach5 T-DNA"
     rep_origin      complement(5924..6512)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6686..7543)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(7544..7648)
                     /label=AmpR promoter
ORIGIN
        1 gaattccaag cttggccaag cttctagaga tctgcaggtc gactctagag gatcctagaa
       61 gctagcttca cgctgccgca agcactcagg gcgcaagggc tgctaaagga agcggaacac
      121 gtagaaagcc agtccgcaga aacggtgctg accccggatg aatgtcagct actgggctat
      181 ctggacaagg gaaaacgcaa gcgcaaagag aaagcaggta gcttgcagtg ggcttacatg
      241 gcgatagcta gactgggcgg ttttatggac agcaagcgaa ccggaattgc cagctggggc
      301 gccctctggt aaggttggga agccctgcaa agtaaactgg atggctttct tgccgccaag
      361 gatctgatgg cgcaggggat caagatcatg agcggagaat taagggagtc acgttatgac
      421 ccccgccgat gacgcgggac aagccgtttt acgtttggaa ctgacagaac cgcaacgttg
      481 aaggagccac tcagccgcgg gtttctggag tttaatgagc taagcacata cgtcagaaac
      541 cattattgcg cgttcaaaag tcgcctaagg tcactatcag ctagcaaata tttcttgtca
      601 aaaatgctcc actgacgttc cataaattcc cctcggtatc caattagagt ctcatattca
      661 ctctcaatcc agatcggggg gcaataagat atgaaaaagc ctgaactcac cgcgacgtct
      721 gtcgagaagt ttctgatcga aaagttcgac agcgtctccg acctgatgca gctctcggag
      781 ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt cctgcgggta
      841 aatagctgcg ccgatggttt ctacaaagat cgttatgttt atcggcactt tgcatcggcc
      901 gcgctcccga ttccggaagt gcttgacatt ggggcattca gcgagagcct gacctattgc
      961 atctcccgcc gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga actgcccgct
     1021 gttctgcagc cggtcgcgga ggccatggat gcgatcgctg cggccgatct tagccagacg
     1081 agcgggttcg gcccattcgg accgcaagga atcggtcaat acactacatg gcgtgatttc
     1141 atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc
     1201 agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga ctgccccgaa
     1261 gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga caatggccgc
     1321 ataacagcgg tcattgactg gagcgaggcg atgttcgggg attcccaata cgaggtcgcc
     1381 aacatcttct tctggaggcc gtggttggct tgtatggagc agcagacgcg ctacttcgag
     1441 cggaggcatc cggagcttgc aggatcgccg cggctccggg cgtatatgct ccgcattggt
     1501 cttgaccaac tctatcagag cttggttgac ggcaatttcg atgatgcagc ttgggcgcag
     1561 ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg tcgggcgtac acaaatcgcc
     1621 cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag tactcgccga tagtggaaac
     1681 cgacgcccca gcactcgtcc gagggcaaag gaatagagta gatgccgacc gggatcttcg
     1741 atccccgatc gttcaaacat ttggcaataa agtttcttaa gattgaatcc tgttgccggt
     1801 cttgcgatga ttatcatata atttctgttg aattacgtta agcatgtaat aattaacatg
     1861 taatgcatga cgttatttat gagatgggtt tttatgatta gagtcccgca attatacatt
     1921 taatacgcga tagaaaacaa aatatagcgc gcaaactagg ataaattatc gcgcgcggtg
     1981 tcatctatgt tactagatcg ggaattgcca agctgatcag attgtcgttt cccgccttcg
     2041 gtttaaacta tcagtgtttg acaggatata ttggcgggta aacctaagag aaaagagcgt
     2101 ttattagaat aatcggatat ttaaaagggc gtgaaaaggt ttatccgttc gtccatttgt
     2161 atgtgcatgc caaccacagg gttcccctcg ggagtgcttg gcattccgtg cgataatgac
     2221 ttctgttcaa ccacccaaac gtcggaaagc ctgacgacgg agcagcattc caaaaagatc
     2281 ccttggctcg tctgggtcgg ctagaaggtc gagtgggctg ctgtggcttg atccctcaac
     2341 gcggtcgcgg acgtagcgca gcgccgaaaa atcctcgatc gcaaatccga cgctgtcgaa
     2401 aagcgtgatc tgcttgtcgc tctttcggcc gacgtcctgg ccagtcatca cgcgccaaag
     2461 ttccgtcaca ggatgatctg gcgcgagttg ctggatctcg ccttcaatcc gggtctgtgg
     2521 cgggaactcc acgaaaatat ccgaacgcag caagatatcg cggtgcatct cggtcttgcc
     2581 tgggcagtcg ccgccgacgc cgttgatgtg gacgccgggc ccgatcatat tgtcgctcag
     2641 gatcgtggcg ttgtgcttgt cggccgttgc tgtcgtaatg atatcggcac cttcgaccgc
     2701 ctgttccgca gaggtgcagg cctcgatctg aaacccgaac cgctggagat tgcgggagca
     2761 gcgagcagta gcctcggggt cgatgtcgta aagtcgtatc cgatcgacgc cgatcagcgc
     2821 cttgaaggcc aaagcctgga actcactttg ggcaccgttg ccgatcagcg ccatcgtgcg
     2881 cgaatcttta cgggccagat actttgccgc gatcgcggag gtcgcggccg ttcgcaaggc
     2941 cgtcaggatt gtcatttccg acagcagcag cggatagccg ctatcgacat cggagagcac
     3001 gccgaacgcg gttactcgag atccggattt tgtagccctg gccgacggcc agcaggtagg
     3061 ccgacaggct catgccggcc gccgccgcct tttcctcaat cgctcttcgt tcgtctggaa
     3121 ggcagtacac cttgataggt gggctgccct tcctggttgg cttggtttca tcagccatcc
     3181 gcttgccctc atctgttacg ccggcggtag ccggccagcc tcgcagagca ggattcccgt
     3241 tgagcaccgc caggtgcgaa taagggacag tgaagaagga acacccgctc gcgggtgggc
     3301 ctacttcacc tatcctgccc ggctgacgcc gttggataca ccaaggaaag tctacacgaa
     3361 ccctttggca aaatcctgta tatcgtgcga aaaaggatgg atataccgaa aaaatcgcta
     3421 taatgacccc gaagcagggt tatgcagcgg aaaagcgctg cttccctgct gttttgtgga
     3481 atatctaccg actggaaaca ggcaaatgca ggaaattact gaactgaggg gacaggcgag
     3541 agacgatgcc aaagagctac accgacgagc tggccgagtg ggttgaatcc cgcgcggcca
     3601 agaagcgccg gcgtgatgag gctgcggttg cgttcctggc ggtgagggcg gatgtcgagg
     3661 cggcgttagc gtccggctat gcgctcgtca ccatttggga gcacatgcgg gaaacgggga
     3721 aggtcaagtt ctcctacgag acgttccgct cgcacgccag gcggcacatc aaggccaagc
     3781 ccgccgatgt gcccgcaccg caggccaagg ctgcggaacc cgcgccggca cccaagacgc
     3841 cggagccacg gcggccgaag caggggggca aggctgaaaa gccggccccc gctgcggccc
     3901 cgaccggctt caccttcaac ccaacaccgg acaaaaagga tctagcgtgg actcaaggct
     3961 ctcgcgaatg gctcgcgttg gaaactttca ttgacacttg aggggcaccg cagggaaatt
     4021 ctcgtccttg cgagaaccgg ctatgtcgtg ctgcgcatcg agcctgcgcc cttggcttgt
     4081 ctcgcccctc tccgcgtcgc tacggggctt ccagcgcctt tccgacgctc accgggctgg
     4141 ttgccctcgc cgctgggctg gcggccgtct atggccctgc aaacgcgcca gaaacgccgt
     4201 cgaagccgtg tgcgagacac cgcggccgcc ggcgttgtgg atacctcgcg gaaaacttgg
     4261 ccctcactga cagatgaggg gcggacgttg acacttgagg ggccgactca cccggcgcgg
     4321 cgttgataga tgaggggcag gctcgatttc ggccggcgac gtggagctgg ccagcctcgc
     4381 aaatcggcga aaacgcctga ttttacgcga gtttcccaca gatgatgtgg acaagcctgg
     4441 ggataagtgc cctgcggtat tgacacttga ggggcgcgac tactgacaga tgaggggcgc
     4501 gatccttgac acttgagggg cagagtgctg acagatgagg ggcgcaccta ttgacatttg
     4561 aggggctgtc cacaggcaga aaatccagca tttgcaaggg tttccgcccg tttttcggcc
     4621 accgctaacc tgtcttttaa cctgctttta aaccaatatt tataaacctt gtttttaacc
     4681 agggctgcgc cctgtgcgcg tgaccgcgca cgccgaaggg gggtgccccc ccttctcgaa
     4741 ccctcccggc ccgctaacgc gggcctccca tccccccagg ggctgcgccc ctcggccgcg
     4801 aacggcctca ccccaaaaat ggcagcgctg gcagtccttg ccattgccgg gatcggggca
     4861 gtaacgggat gggcgatcag cccgagcgcg acgcccggaa gcattgacgt gccgcaggtg
     4921 ctggcatcga cattcagcga ccaggtgccg ggcagtgagg gcggcggcct gggtggcggc
     4981 ctgcccttca cttcggccgt cggggcattc acggacttca tggcggggcc ggcaattttt
     5041 accttgggca ttcttggcat agtggtcgcg ggtgccgtgc tcgtgttcgg gggtgaattg
     5101 caagctagct tgcttggtcg ttccggtacc gtgaacgtcg gctcgattgt acctgcgttc
     5161 aaatactttg cgatcgtgtt gcgcgcctgc ccggtgcgtc ggctgatctc acggatcgac
     5221 tgcttctctc gcaacgccat ccgacggatg atgtttaaaa gtcccatgtg gatcactccg
     5281 ttgccccgtc gctcaccgtg ttggggggaa ggtgcacatg gctcagttct caatggaaat
     5341 tatctgccta accggctcag ttctgcgtag aaaccaacat gcaagctcca ccgggtgcaa
     5401 agcggcagcg gcggcaggat atattcaatt gtaaatggct tcatgtccgg gaaatctaca
     5461 tggatcagca atgagtatga tggtcaatat ggagaaaaag aaagagtaat taccaatttt
     5521 ttttcaattc aaaaatgtag atgtccgcag cgttattata aaatgaaagt acattttgat
     5581 aaaacgacaa attacgatcc gtcgtattta taggcgaaag caataaacaa attattctaa
     5641 ttcggaaatc tttatttcga cgtgtctaca ttcacgtcca aatgggggct tagatgagaa
     5701 acttcacgat cgatgggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     5761 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     5821 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     5881 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     5941 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     6001 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     6061 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     6121 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     6181 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     6241 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     6301 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     6361 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     6421 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     6481 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     6541 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     6601 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     6661 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     6721 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     6781 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     6841 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     6901 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     6961 cgccagttaa tagtttgcgc aacgttgttt gcattgctac aggcatcgtg gtgtcacgct
     7021 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     7081 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     7141 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     7201 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     7261 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     7321 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     7381 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     7441 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     7501 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     7561 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     7621 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgcgc
     7681 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     7741 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     7801 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     7861 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     7921 cctgatagac ggtttttcgc cc
//
