pGALD7DLFN vector (V006098)

LOCUS       V006098                 4719 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V006098
VERSION     V006098
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4719)
  AUTHORS   Loset GA, Roos N, Bogen B, Sandlie I.
  TITLE     Expanding the versatility of phage display II: improved affinity
            selection of folded domains on protein VII and IX of the filamentous
            phage
  JOURNAL   PLoS ONE 6 (2), E17433 (2011)
   PUBMED   21390283
REFERENCE   2  (bases 1 to 4719)
  AUTHORS   Loset GA, Roos N, Bogen B, Sandlie I.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-OCT-2010) Department of Molecular Biosciences,
            University of Oslo, PO Box 1041 Blindern, Oslo N-0316, Norway
REFERENCE   3  (bases 1 to 4719)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4719)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE";
            date: "2011"; volume: "6"; issue: "2"; pages: "E17433"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (10-OCT-2010) Department of Molecular Biosciences, University of
            Oslo, PO Box 1041 Blindern, Oslo N-0316, Norway"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4719
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    31..52
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        67..97
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    105..121
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             140..151
                     /note="strong bacterial ribosome binding site (Elowitz and
                     Leibler, 2000)"
     misc_feature    164..907
                     /gene="scTCR-pVII fusion"
                     /label="Region: scTCR"
                     /note="Region: scTCR"
     CDS             935..1033
                     /label="M13 gene VII"
                     /note="pVII"
     terminator      complement(1057..1100)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             complement(1121..1144)
                     /label="FLAG"
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     CDS             complement(1145..1954)
                     /gene="fkpA"
                     /label="FKBP-type peptidyl-prolyl cis-trans isomerase FkpA"
                     /note="FKBP-type peptidyl-prolyl cis-trans isomerase FkpA
                     from Escherichia coli (strain K12). Accession#: P45523"
     rep_origin      2252..2707
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2733..2837
                     /label="AmpR promoter"
     CDS             2838..3695
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      3869..4457
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"