LOCUS       V006098                 4719 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V006098
VERSION     V006098
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4719)
  AUTHORS   Loset GA, Roos N, Bogen B, Sandlie I.
  TITLE     Expanding the versatility of phage display II: improved affinity
            selection of folded domains on protein VII and IX of the filamentous
            phage
  JOURNAL   PLoS ONE 6 (2), E17433 (2011)
   PUBMED   21390283
REFERENCE   2  (bases 1 to 4719)
  AUTHORS   Loset GA, Roos N, Bogen B, Sandlie I.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-OCT-2010) Department of Molecular Biosciences,
            University of Oslo, PO Box 1041 Blindern, Oslo N-0316, Norway
REFERENCE   3  (bases 1 to 4719)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4719)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE";
            date: "2011"; volume: "6"; issue: "2"; pages: "E17433"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (10-OCT-2010) Department of Molecular Biosciences, University of
            Oslo, PO Box 1041 Blindern, Oslo N-0316, Norway"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4719
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    31..52
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        67..97
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    105..121
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             140..151
                     /note="strong bacterial ribosome binding site (Elowitz and
                     Leibler, 2000)"
     misc_feature    164..907
                     /gene="scTCR-pVII fusion"
                     /label="Region: scTCR"
                     /note="Region: scTCR"
     CDS             935..1033
                     /label="M13 gene VII"
                     /note="pVII"
     terminator      complement(1057..1100)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             complement(1121..1144)
                     /label="FLAG"
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     CDS             complement(1145..1954)
                     /gene="fkpA"
                     /label="FKBP-type peptidyl-prolyl cis-trans isomerase FkpA"
                     /note="FKBP-type peptidyl-prolyl cis-trans isomerase FkpA
                     from Escherichia coli (strain K12). Accession#: P45523"
     rep_origin      2252..2707
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2733..2837
                     /label="AmpR promoter"
     CDS             2838..3695
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      3869..4457
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 ctcgagagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
       61 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
      121 atttcacaca gaattcatta aagaggagaa attaaccatg gcccagcaga aggtgcagca
      181 gagcccagaa tccctcattg tcccagaggg agccatgact tctctcaact gcactttcag
      241 cgacagtgct tctcagtatt ttgcatggta cagacagcaa tctgggaaag cccccaaggc
      301 actgatgtcc atcttctcca atggtgaaaa agaagaaggc agattcacaa ttcacctcaa
      361 taaagccagt ctgcatttct cgctacacat cagagactcc cagcccagtg actctgctct
      421 ctacctctgt gcagtgaggg gccctaatac aggaaactac aaatacgtct ttggagcagg
      481 taccagactg aaggttatag caaagctttc agggagtgca tccgccccaa aacttgaaga
      541 aggtgaattt tcagaagcac gcgtagaggc tgcagtcacc caaagcccaa gaaacaaggt
      601 ggcagtaaca ggaggaaagg tgacattgag ctgtaatcag actaataacc acaacaacat
      661 gtactggtat cggcaggaca cggggcatgg gctgaggctg atccattatt catatggtgc
      721 tggcagcact gagaaaggag atatccctga tggatacaag gcctccagac caagccaaga
      781 gaacttctcc ctcattctgg agttggctac cccctctcag acatcagtgt acttctgtgc
      841 cagcggtgat gcgggacagg ggcactccga ctacaccttc ggctcaggga ccaggctttt
      901 ggtaatagcg gccgctggat ccaaagatat cagaatggag caggtcgcgg atttcgacac
      961 aatttatcag gcgatgatac aaatctccgt tgtactttgt ttcgcgcttg gtataatcgc
     1021 tgggggtcaa agatgataag ctagcttgag gcatcaataa aacgaaaggc tcagtcgaaa
     1081 gactgggcct ttcattttat ctgttgtttg tcggttaacg cttgtcgtca tcgtccttgt
     1141 agtctttttt agcagaatct gcggctttcg catcagcttc cggctttgca tcagccttcg
     1201 gcgctggttt cacatccagc agctctacgt caaacaccag ggtagaattc ggtgggatcc
     1261 ccggaacacc cgctttgccg taagccagtt ctggtggaat aaccagtttg atcttaccgc
     1321 ctttcttgat gttcttcaga ccttctgtcc aacccgggat aacaccgtcc agacggaaag
     1381 aaagcggttc accacgggtg taagagttgt cgaactcttt accgtcgatc agcgtacctt
     1441 tgtagttcac tacaacagta tcgctgtctt tcggtgcttc gcctttaccg gcttctacta
     1501 cctgataaac cagaccagtt gaagaggttt tcacaccttt ctctttggca aatttctcgc
     1561 ggtactcttt accttttgct tcgttatcag ccgcgtcttt ttccatcttc gcctgagcag
     1621 aagacttcac gcgagcttcg aatgcttgta gagtctgttc gatctcttgg tcggagagtt
     1681 tgctcttatc agcaaatgca tcctgaacac cagcgatcag ctgatcttta tccagtttga
     1741 tgcccagttt ttcttgttct tttagagagt tttccatgta acgacccagc gaggcaccca
     1801 gtgcataagc tgatttctga tcgtcatttt tgaacgctgc tttgctgtca gcagctgtag
     1861 caggttttgc agcttcagca gcaaaagtga ttggtgcatg cagggcaacg gccattgtgg
     1921 tcgccagcag cgttacttta aacagtgatt tcatccatat ctccaggatc ggggcatctc
     1981 accccagggt taactattat cagaagggta ctataaagcg ttgtcgaaca aatctacata
     2041 cagacacgcc ctattatcat ctattttcag actctttttg tttaaattag tttcgatgac
     2101 cgcgaaatga gtgctgtctc gggcagcaaa gttaagtaga atccgcggcg accattcgac
     2161 aaaagaggtg aasagtcgac ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     2221 caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg
     2281 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     2341 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     2401 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     2461 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     2521 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     2581 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     2641 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
     2701 acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     2761 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     2821 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     2881 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     2941 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     3001 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     3061 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     3121 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     3181 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     3241 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     3301 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     3361 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     3421 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     3481 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     3541 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     3601 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     3661 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     3721 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     3781 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     3841 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     3901 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     3961 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
     4021 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     4081 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     4141 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     4201 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     4261 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     4321 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     4381 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     4441 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     4501 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     4561 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     4621 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     4681 ttcattaatg caggtatcac gaggcccttt cgtcttcac
//
