Basic Vector Information
- Vector Name:
- pGal4-p65f1-539
- Antibiotic Resistance:
- Ampicillin
- Length:
- 6147 bp
- Type:
- Mammalian expression vector
- Replication origin:
- ori
- Source/Author:
- De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman Fonseca M, Vanhoucke M, Beyaert R.
- Promoter:
- RSV
pGal4-p65f1-539 vector Map
pGal4-p65f1-539 vector Sequence
LOCUS V006110 6147 bp DNA circular SYN 18-DEC-2018
DEFINITION Exported.
ACCESSION V006110
VERSION V006110
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
.
REFERENCE 1 (bases 1 to 6147)
AUTHORS De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
Fonseca M, Vanhoucke M, Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 6147)
AUTHORS De Schamphelaire W.
TITLE Direct Submission
JOURNAL Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM
REFERENCE 3 (bases 1 to 6147)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 6147)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
9052, BELGIUM"
SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..6147
/mol_type="other DNA"
/organism="synthetic DNA construct"
polyA_signal complement(766..900)
/label="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
CDS complement(924..2540)
/gene="RELA"
/label="Transcription factor p65"
/note="Transcription factor p65 from Homo sapiens.
Accession#: Q04206"
misc_feature complement(2541..2576)
/label="link"
/note="link"
CDS complement(2577..3017)
/label="GAL4 DNA binding domain"
/note="DNA binding domain of the GAL4 transcriptional
activator"
promoter complement(3159..3420)
/label="RSV promoter"
/note="Rous sarcoma virus enhancer/promoter"
promoter 3745..4074
/label="SV40 promoter"
/note="SV40 enhancer and early promoter"
rep_origin complement(4320..4908)
/direction=LEFT
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(5082..5939)
/label="AmpR"
/note="beta-lactamase"
promoter complement(5940..6044)
/label="AmpR promoter"
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