LOCUS       V006110                 6147 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V006110
VERSION     V006110
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6147)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6147)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 6147)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6147)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6147
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    complement(766..900)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     CDS             complement(924..2540)
                     /gene="RELA"
                     /label="Transcription factor p65"
                     /note="Transcription factor p65 from Homo sapiens.
                     Accession#: Q04206"
     misc_feature    complement(2541..2576)
                     /label="link"
                     /note="link"
     CDS             complement(2577..3017)
                     /label="GAL4 DNA binding domain"
                     /note="DNA binding domain of the GAL4 transcriptional
                     activator"
     promoter        complement(3159..3420)
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     promoter        3745..4074
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     rep_origin      complement(4320..4908)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5082..5939)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(5940..6044)
                     /label="AmpR promoter"
ORIGIN
        1 ttcctttgcc taatttaaat gaggacttaa cctgtggaaa tattttgatg tgggaagctg
       61 ttactgttaa aactgaggtt attggggtaa ctgctatgtt aaacttgcat tcagggacac
      121 aaaaaactca tgaaaatggt gctggaaaac ccattcaagg gtcaaatttt catttttttg
      181 ctgttggtgg ggaacctttg gagctgcagg gtgtgttagc aaactacagg accaaatatc
      241 ctgctcaaac tgtaacccca aaaaatgcta cagttgacag tcagcagatg aacactgacc
      301 acaaggctgt tttggataag gataatgctt atccagtgga gtgctgggtt cctgatccaa
      361 gtaaaaatga aaacactaga tattttggaa cctacacagg tggggaaaat gtgcctcctg
      421 ttttgcacat tactaacaca gcaaccacag tgcttcttga tgagcagggt gttgggccct
      481 tgtgcaaagc tgacagcttg tatgtttctg ctgttgacat ttgtgggctg tttaccaaca
      541 cttctggaac acagcagtgg aagggacttc ccagatattt taaaattacc cttagaaagc
      601 ggtctgtgaa aaacccctac ccaatttcct ttttgttaag tgacctaatt aacaggagga
      661 cacagagggt ggatgggcag cctatgattg gaatgtcctc tcaagtagag gaggttaggg
      721 tttatgagga cacagaggag cttcctgggg atcgctcgat cgagcgatcc agacatgata
      781 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
      841 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
      901 aattccgcca cggatcaagc ttagtccgca atggaggaga agtcttcatc tcctgaaagg
      961 aggccattgg ggagccccgg ggcccccagt ggagcaggag ctgggtcggg gggcctctgg
     1021 gcccctgtca ctaggcgagt tatagcctca gggtactcca tcagcatggg ctcagttgtg
     1081 tggggggcca caggtatgcc ctggttcagc agctgctgaa actcggagtt gtcgacggat
     1141 gccaggtctg tgaacacagc tgggtctgtg ctgttgccaa gcaaggcccc caggtcttca
     1201 tcatcaaact gcagctgcag cagggcctct gacagcgttc cttccccagc ctgggtgggc
     1261 ttgggggcag gtggggccac agcctgagga gggcctgggg ctaggactgg gacaggggct
     1321 ggggcctggg ccagagctga taccatggct ggagcagggg caggggctgg agcctggggc
     1381 aggacttggg gaggggccgg ggccaaggcc gaggcctggc tgatctgccc agaaggaaac
     1441 accatggtgg gaaactcatc atagttgatg gtgctcaggg atgacgtaaa gggatagggc
     1501 tggggtgctg gcttggggac agaagctgag ctgcgggaag gcacagcaat gcgtcgaggt
     1561 ggaggccggg ggtcggtggg tccgctgaaa ggactcttct tcatgatgct cttgaaggtc
     1621 tcatatgtcc ttttacgttt ctcctcaatc cggtgacgat cgtctgtatc tggcaggtac
     1681 tggaattcca tgggctcact gagctcccgg tcggaaggcc gccgcagctg catggagaca
     1741 cgcacaggag cctgcaggct ggggtctgcg tagggagggg tccggaacac aatggccact
     1801 tgtcggtgca catcagcttg cgaaaaggag cctcgggcct cccagcctgg tcccgtgaaa
     1861 tacacctcaa tgtcctcttt ctgcaccttg tcacacagta ggaagatctc atccccaccg
     1921 aggcagctgc cagagtttcg gttcactcgg cagatcttga gctcggcagt gttgggggca
     1981 cgattgtcaa agatgggatg aggaaggaca ggcggcaggc ggaggggcct gcctgatggg
     2041 tcccgcactg tcacctggaa gcagagccgc acagcattca ggtcgtagtc cccacgctgc
     2101 tcttctatag gaacttggaa ggggttgttg ttggtctgga tgcgctgact gatagcctgc
     2161 tccaggtccc gcttcttcac acactggatt cccaggttct ggaaactgtg gatgcagcgg
     2221 tccgggcaga gctcagcctc atagaagcca tcccggcagt cctttcctac aagctcgtgg
     2281 gggtgaggcc ggtgaggagg gtccttggtg accagggaga tgcgcactgt ccctggtcct
     2341 gtgtagccat tgatcttgat ggtggggtgg gtcttggtgg tatctgtgct cctctcgcct
     2401 gggatgctgc ccgcggagcg cccctcgcac ttgtagcgga agcgcatgcc ccgctgcttg
     2461 ggctgctcaa tgatctccac ataggggcca gaggcctggg ctggctctgc cgggaagatg
     2521 agggggaaca gttcgtccat ggccggggtc ccctggtcga cacccgggaa ttccggcgat
     2581 acagtcaact gtctttgacc tttgttacta ctctcttccg atgatgatgt cgcacttatt
     2641 ctatgctgtc tcaatgttag aggcatatca gtctccactg aagccaatct atctgtgacg
     2701 gcatctttat tcacattatc ttgtacaaat aatcctgtta acaatgcttt tatatcctgt
     2761 aaagaatcca ttttcaaaat catgtcaagg tcttctcgag gaaaaatcag tagaaatagc
     2821 tgttccagtc tttctagcct tgattccact tctgtcagat gtgccctagt cagcggagac
     2881 cttttggttt tgggagagta gcgacactcc cagttgttct tcagacactt ggcgcacttc
     2941 ggtttttctt tggagcactt gagcttttta agtcggcaaa tatcgcatgc ttgttcgata
     3001 gaagacagta gcttcagatc tggctgctgg gagtccatca gtgaatatca actctggcag
     3061 aggagccgct cgcccgccac cgtccgcagt tcccgccgca gccgagataa acaacttagc
     3121 ttgtgaacgc agaaggagca ggaggtaccc agcttggagg tgcacaccaa tgtggtgaat
     3181 ggtcaaatgg cgtttattgt atcgagctag gcacttaaat acaatatctc tgcaatgcgg
     3241 aattcagtgg ttcgtccaat ccatgtcaga cctgtctgtt gccttcctaa taaggcacga
     3301 tcgtaccacc ttacttccac caatcggcat gcacggtgct ttttctctcc ttgtaaggca
     3361 tgttgctaac tcatcgttac catgttgcaa gactacaagt gtattgcata agactacatt
     3421 tccccctccc tatgcaaaag cgaaactact atatcctgag gggactccta accgcgtaca
     3481 accgaagccc cgcttttcgc ctaaacacac cctagtcccc tcagatacgc gtatatctgg
     3541 cccgtacatc gcgaagcagc gcaaaacgcc taaccctaag cagattcttc atgcaattgt
     3601 cggtcaagcc ttgccttgtt gtagcttaaa ttttgctcgc gcactactca gcgacctcca
     3661 acacacaagc agggagcaga tactggctta actatgcggc atcagagcag attgtactga
     3721 gagtgcacca tatggctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
     3781 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
     3841 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     3901 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     3961 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     4021 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc
     4081 catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct
     4141 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     4201 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     4261 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     4321 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     4381 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     4441 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     4501 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     4561 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     4621 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     4681 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     4741 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
     4801 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     4861 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     4921 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     4981 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     5041 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     5101 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     5161 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     5221 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     5281 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     5341 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctgcaggc
     5401 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     5461 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     5521 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     5581 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     5641 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaacacgg
     5701 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     5761 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     5821 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     5881 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     5941 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     6001 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     6061 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
     6121 atcacgaggc cctttcgtct tcaagaa
//
