pFY20 vector (V006148)

LOCUS       40924_20761        6807 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pFY20, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6807)
  AUTHORS   Wahls WP, Davidson MK.
  TITLE     Low-copy episomal vector pFY20 and high-saturation coverage genomic 
            libraries for the fission yeast Schizosaccharomyces pombe
  JOURNAL   Yeast 25 (9), 643-650 (2008)
  PUBMED    18613214
REFERENCE   2  (bases 1 to 6807)
  AUTHORS   Wahls WP, Davidson MK.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2008) Biochemistry and Molecular Biology, 
            University of Arkansas for Medical Sciences, 4301 West Markham 
            Street (slot 516), Little Rock, AR 72205-7199, USA
REFERENCE   3  (bases 1 to 6807)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6807)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast"; 
            date: "2008"; volume: "25"; issue: "9"; pages: "643-650"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (21-APR-2008) Biochemistry and Molecular Biology, University of 
            Arkansas for Medical Sciences, 4301 West Markham Street (slot 516), 
            Little Rock, AR 72205-7199, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6807
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    237..1551
                     /note="stb; plasmid stability element derived from 
                     Schizosaccharomyces pombe"
     rep_origin      1900..2681
                     /label=ars1
                     /note="Schizosaccharomyces pombe autonomously replicating 
                     sequence ars1"
     misc_feature    2749..2804
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     CDS             3332..4123
                     /label=S. pombe ura4
                     /note="orotidine 5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     primer_bind     complement(4586..4602)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(4610..4626)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4634..4664)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4679..4700)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4988..5576)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5750..6607)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(6608..6712)
                     /label=AmpR promoter