LOCUS       40924_20761        6807 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pFY20, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6807)
  AUTHORS   Wahls WP, Davidson MK.
  TITLE     Low-copy episomal vector pFY20 and high-saturation coverage genomic 
            libraries for the fission yeast Schizosaccharomyces pombe
  JOURNAL   Yeast 25 (9), 643-650 (2008)
  PUBMED    18613214
REFERENCE   2  (bases 1 to 6807)
  AUTHORS   Wahls WP, Davidson MK.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2008) Biochemistry and Molecular Biology, 
            University of Arkansas for Medical Sciences, 4301 West Markham 
            Street (slot 516), Little Rock, AR 72205-7199, USA
REFERENCE   3  (bases 1 to 6807)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6807)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast"; 
            date: "2008"; volume: "25"; issue: "9"; pages: "643-650"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (21-APR-2008) Biochemistry and Molecular Biology, University of 
            Arkansas for Medical Sciences, 4301 West Markham Street (slot 516), 
            Little Rock, AR 72205-7199, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6807
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    237..1551
                     /note="stb; plasmid stability element derived from 
                     Schizosaccharomyces pombe"
     rep_origin      1900..2681
                     /label=ars1
                     /note="Schizosaccharomyces pombe autonomously replicating 
                     sequence ars1"
     misc_feature    2749..2804
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     CDS             3332..4123
                     /label=S. pombe ura4
                     /note="orotidine 5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     primer_bind     complement(4586..4602)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(4610..4626)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4634..4664)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4679..4700)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4988..5576)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5750..6607)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(6608..6712)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 gcggccgcga attaattctc cgtttgatca cggaaaagtc tattgaagag aacattcctt
      301 tctcgagctc agtataagct tgaccttgat ggaaaagtta tccaagctgg taagtttgac
      361 aacaagtcta cacctgagga gcgtgaagct tttttacgtt ctttacttga acatgatggc
      421 gacgatgatc atgacttaac ttacggcgaa cttcaagatg atgagttgaa cgagttgatt
      481 tctagaacag atgaagagct cgtacttttt aaaaaattag ataaagaacg tgcagctacc
      541 gatatatatg gtaaaggtaa accgctggaa cgtttactta ctgtaaatga acttcctgac
      601 ttttataaag tcgaggttga cagttttgca gttcaaagct ctagtgagtt ggaggatcag
      661 tatctcgaaa gaaagcgacg tcgtcgtaat tctatcagtt atacggaatt aaccctcgac
      721 gaattaaata cagttgacga tccatcttcc actctaatgc cgcgtaaaag aggtcgaccg
      781 agaaaaaaaa caaactcagg ctctagtcta tcaacccctt tatcacaaga atccagtttg
      841 gccagaagtg gtcgaaaaaa tacaccttca tataagcaga aggcacttag aagatattgt
      901 atggaaatct ttgaaaggct gtataatctt caaagtgaag acggcagatt cgttaatggt
      961 cttttccttt atcctcccaa ccgcaaactt taccctgatt actatataat aattaaacga
     1021 cccatagctc ttggaaaaat taaaaggaat attaagaatg accgttatgg agatgttgga
     1081 gaactgattg cagattttat gctcatgttt aataatgcat atacctataa tgaagagcat
     1141 tcaattgttt acgaggatgc aaaattaatg gaaaaaactc taaaggaggt gattgaagat
     1201 ttagaaaaaa acaattcttt gcatgcatat gaggaagagg ctttaaatga ggaacaagca
     1261 tctcttgtct ttttggaaaa ctctgaagca gagctaccgt tggattctgg tatagtaagc
     1321 gccgaagatg acaagttatt acttatgaag attcttcttc ttcttattcg gagtaggact
     1381 acgtttacct ttgtcgttta gtttaatgcc ttgttataat attttggtac attttgttag
     1441 gtgaggacta ttggcgactt caaagctcag tgaagactta gtcaatcacg agttattctt
     1501 ctaaagtgtc taagcctgta taatatatat gtttagtttc ttaaaagaat taattccaac
     1561 cttccaattc attaaatcga gtcctccatc agatgattta caaggttttg catagaatcc
     1621 ctaagtaaga gaagttgtta gtgatttctt caagtttact aaaatttaca tacaaatgga
     1681 tgatctcttc tccattgttt cctttcttct tgcaaacgcg ttttacaaag agatgacatg
     1741 ctattttctg attatttttt tctatagttt tctattttgt acgtccttgg gagtccgaaa
     1801 tgtaaaatcg gggtatggtt agtagcggca gcgttcatta tggtatataa aagataaaaa
     1861 gtaggggcaa gcgagacaga atgggataca agggcatcgt ctataattat agctaaaaat
     1921 tgtattttaa tttgtatttt ttgtaatttt atttctcatt gttttactta aaatgaatgc
     1981 gaattagaga aaacttcaac gaaatgtcaa aataagctca gcaaaatata caattttagg
     2041 gaaagcgatc agcaacttat tctccgttgt agattctttt tcttcataaa ttccaacaat
     2101 gtaactgcca attgattttt ctctagatgc taataaattg aaactaatga atataataac
     2161 acaggttttg ataaattgta aactcttact caaaaaaaga atgttaaaac taatttaacc
     2221 tttttataaa ggctcatggc gttaaagagg atagtaaaaa ttctacaatt ttttaattta
     2281 atgtcacaat tgtcttgcga atataattct attaattttc gctaactgct gttttagtac
     2341 tattactttc aatatgcctt atatgcaact ttaattcatg aagaatgcaa tatttttgca
     2401 atctttcttg tactgagcgc tttttatcat ttagtactca ttatttaatt tttcagtaaa
     2461 gggcaataaa taaatttgta gaagatgcaa tgtaatctcc tctatccttt tgctcatatg
     2521 tttatgagta tacctagtct agaaaggctt gtatttaaaa tattattcaa taaaaattca
     2581 caatttttac gacatgtgct accattcact taacttcctg attataaaat tggttcgttt
     2641 atactaatta cttaagtacc tttaactaaa caaaatgcct atatatatat taatttacaa
     2701 tgagtgtcag ataagtcact atgtccgagt ggttaaggag ttagactcga attcgagctc
     2761 ggtacccggg gatcctctag agtcgacctg caggcatgca gcttagctac aaatcccact
     2821 ggctatatgt atgcatttgt gttaaaaaag tttgtataga ttatttaatc tactcagcat
     2881 tctttctcta aataggaatt tgttacttaa tggagaaaaa aatgtttcga tttacctagt
     2941 gtatttgttt gtatactcac gtttaatttc aaacatccat tctatcttgt gtaatttttg
     3001 gcatggtgaa aaagataatc agccttataa tctttacaaa agtaagaaat tctgtaaata
     3061 agccttaatg cccttgcttt aaattaaaat ggttcttttt catgataatg tttgcacttt
     3121 gtgaatatat tttagatagt tctgtgaggt ataattaaga tgttttagag acttatacaa
     3181 ttttgtcttt ataaattctt aattgatttt accatcccag tttaactatg cttcgtcggc
     3241 atctctgcac atgtcgtgtt ttcttaccgt attgtcctac caagaacctc ttttttgctt
     3301 ggatcgaaat taaaggttta aaagcaaagt tatggatgct agagtatttc aaagctattc
     3361 agctagagct gaggggatga aaaatcccat tgccaaggaa ttgttggctt tgatggaaga
     3421 aaagcaaagc aacttgtcag tcgcggtcga tttgacgaag aaatccgaaa tcttagaatt
     3481 ggtagataaa attggaccct atgtctgtgt tatcaagaca catattgacg ttgtcgagga
     3541 tttcgaccag gatatggtag aaaaactggt ggccttaggt aaaaagcatc gttttcttat
     3601 ctttgaggat cgcaaattcg cagacattgg aaataccgtc aagctacaat atgcatctgg
     3661 tgtgtacaaa attgcttctt gggctcatat cacaaattgc catacagtgc caggcgaggg
     3721 tattatacaa ggcctcaaag aagttggttt acctttggga cgtggtctct tgcttttggc
     3781 tgaaatgtct tccaaaggct ctttggctac tggttcctac acagagaaaa ccttagaatg
     3841 gtttgagaag cataccgatt tttgctttgg ctttatagct ggtcgtcgat ttcctaacct
     3901 tcaaagcgac tacataacta tgtcccctgg tatcggcttg gatgttaaag gagacgggct
     3961 gggacagcaa tatcgtactc ctgaagaagt gattgtaaac tgcggtagcg atatcatcat
     4021 tgttggtcgt ggagtctatg gagctggtcg taatcctgtt gtcgaagcca agagatatag
     4081 agaagctggt tggaaggcat atcagcaaag actttctcag cattaaaaaa agactaatgt
     4141 aaaatttttt tggttggtta ttgaaaaagt cgatgccttg tttgcgtttg ttttcctagg
     4201 cgttttatgt cagaaggcat ttagaattag tatacaagta ctctttggta aaattttatg
     4261 tagcgactaa aatattaact attatagata aacaccttgg gaataaaaag taatttgcta
     4321 tagtaattta ttaaacatgc tcctacaaca ttaccacaat cttttctctt ggattgacat
     4381 tgaataagaa aagagtgaat ttttttagac ttgtaatgat aactatgtac aaagccaatg
     4441 aaagatgtat gtagatgaat gtaaaatacc atgtagacaa acaagataaa acttggttat
     4501 aaacattggt gttggaacag aataaattag atgtcaaaaa gtttcgtcaa tatcacaagc
     4561 tgcatgcaag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     4621 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     4681 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     4741 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     4801 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     4861 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     4921 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     4981 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     5041 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     5101 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     5161 cgggaagcgt ggcgctttct caatgctcac gctgtaggta tctcagttcg gtgtaggtcg
     5221 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     5281 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     5341 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     5401 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
     5461 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     5521 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     5581 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     5641 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     5701 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     5761 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     5821 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     5881 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     5941 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     6001 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     6061 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     6121 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     6181 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     6241 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     6301 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     6361 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     6421 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     6481 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     6541 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     6601 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     6661 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     6721 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
     6781 ataggcgtat cacgaggccc tttcgtc
//
