pFUSE-mFc2-adapt-scFv vector (V006150)

LOCUS       40924_20736        4214 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pFUSE-mFc2-adapt-scFv, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4214)
  AUTHORS   Moutel S, El Marjou A, Vielemeyer O, Nizak C, Benaroch P, Dubel S, 
            Perez F.
  TITLE     A multi-Fc-species system for recombinant antibody production
  JOURNAL   BMC Biotechnol. 9, 14 (2009)
  PUBMED    19245715
REFERENCE   2  (bases 1 to 4214)
  AUTHORS   Perez F, Moutel S.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2009) CNRS UMR144, Institut Curie / CNRS, 26, rue 
            d'Ulm, Pa, Paris 75005, France
REFERENCE   3  (bases 1 to 4214)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4214)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol. 9, 14 (2009)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (09-FEB-2009) CNRS UMR144, Institut Curie / CNRS, 26, rue d'Ulm, Pa,
            Paris 75005, France"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4214
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        35..246
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             259..527
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
     sig_peptide     577..636
                     /label=IL-2 signal sequence
                     /note="human interleukin-2 signal sequence"
     misc_feature    644..649
                     /note="NcoI restriction site: upstream insertion site for
                     scFv"
     misc_feature    661..668
                     /note="NotI restriction site; downstream insertion site for
                     scFv"
     misc_feature    676..1359
                     /note="Fc domain derived from Mus musculus immunoglobulin
                     IgG2"
     polyA_signal    complement(1379..1500)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    complement(1604..1998)
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
     CDS             complement(2015..2386)
                     /label=BleoR
                     /note="antibiotic-binding protein"
     enhancer        complement(2979..3282)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(3362..3950)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     polyA_signal    4062..4110
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    4121..4212
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"