LOCUS       40924_20736        4214 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pFUSE-mFc2-adapt-scFv, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4214)
  AUTHORS   Moutel S, El Marjou A, Vielemeyer O, Nizak C, Benaroch P, Dubel S, 
            Perez F.
  TITLE     A multi-Fc-species system for recombinant antibody production
  JOURNAL   BMC Biotechnol. 9, 14 (2009)
  PUBMED    19245715
REFERENCE   2  (bases 1 to 4214)
  AUTHORS   Perez F, Moutel S.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2009) CNRS UMR144, Institut Curie / CNRS, 26, rue 
            d'Ulm, Pa, Paris 75005, France
REFERENCE   3  (bases 1 to 4214)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4214)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol. 9, 14 (2009)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (09-FEB-2009) CNRS UMR144, Institut Curie / CNRS, 26, rue d'Ulm, Pa,
            Paris 75005, France"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4214
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        35..246
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             259..527
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
     sig_peptide     577..636
                     /label=IL-2 signal sequence
                     /note="human interleukin-2 signal sequence"
     misc_feature    644..649
                     /note="NcoI restriction site: upstream insertion site for
                     scFv"
     misc_feature    661..668
                     /note="NotI restriction site; downstream insertion site for
                     scFv"
     misc_feature    676..1359
                     /note="Fc domain derived from Mus musculus immunoglobulin
                     IgG2"
     polyA_signal    complement(1379..1500)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    complement(1604..1998)
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
     CDS             complement(2015..2386)
                     /label=BleoR
                     /note="antibiotic-binding protein"
     enhancer        complement(2979..3282)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(3362..3950)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     polyA_signal    4062..4110
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    4121..4212
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 cgaaggatct gcgatcgctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc
       61 cccgagaagt tggggggagg ggtcggcaat tgaacgggtg cctagagaag gtggcgcggg
      121 gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa
      181 ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga
      241 acacagctga agcttcgagg ggctcgcatc tctccttcac gcgcccgccg ccctacctga
      301 ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc ccgcctgtgg tgcctcctga
      361 actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga gaccgggcct ttgtccggcg
      421 ctcccttgga gcctacctag actcagccgg ctctccacgc tttgcctgac cctgcttgct
      481 caactctacg tctttgtttc gttttctgtt ctgcgccgtt acagatccaa gctgtgaccg
      541 gcgcctacct gagatcaccg gcgaaggagg gccaccatgt acaggatgca actcctgtct
      601 tgcattgcac taagtcttgc acttgtcacg aattcgatat cggccatggt ttggtacctt
      661 gcggccgcta gatctcctcc actcaaagag tgtcccccat gcgcagctcc agacctcttg
      721 ggtggaccat ccgtcttcat cttccctcca aagatcaagg atgtactcat gatctccctg
      781 agccctatgg tcacatgtgt ggtggtggat gtgagcgagg atgacccaga cgtccagatc
      841 agctggtttg tgaacaacgt ggaagtacac acagctcaga cacaaaccca tagagaggat
      901 tacaacagta ctctccgggt ggtcagtgcc ctccccatcc agcaccagga ctggatgagt
      961 ggcaaggagt tcaaatgcaa ggtcaacaac agagccctcc catcccccat cgagaaaacc
     1021 atctcaaaac ccagagggcc agtaagagct ccacaggtat atgtcttgcc tccaccagca
     1081 gaagagatga ctaagaaaga gttcagtctg acctgcatga tcacaggctt cttacctgcc
     1141 gaaattgctg tggactggac cagcaatggg cgtacagagc aaaactacaa gaacaccgca
     1201 acagtcctgg actctgatgg ttcttacttc atgtacagca agctcagagt acaaaagagc
     1261 acttgggaaa gaggaagtct tttcgcctgc tcagtggtcc acgagggtct gcacaatcac
     1321 cttacgacta agaccatctc ccggtctctg ggtaaatgag ctagctggcc agacatgata
     1381 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
     1441 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
     1501 aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt
     1561 taaagcaagt aaaacctcta caaatgtggt atggaattaa ttctaaaata cagcatagca
     1621 aaactttaac ctccaaatca agcctctact tgaatccttt tctgagggat gaataaggca
     1681 taggcatcag gggctgttgc caatgtgcat tagctgtttg cagcctcacc ttctttcatg
     1741 gagtttaaga tatagtgtat tttcccaagg tttgaactag ctcttcattt ctttatgttt
     1801 taaatgcact gacctcccac attccctttt tagtaaaata ttcagaaata atttaaatac
     1861 atcattgcaa tgaaaataaa tgttttttat taggcagaat ccagatgctc aaggcccttc
     1921 ataatatccc ccagtttagt agttggactt agggaacaaa ggaaccttta atagaaattg
     1981 gacagcaaga aagcgagctt ctagcttatc ctcagtcctg ctcctctgcc acaaagtgca
     2041 cgcagttgcc ggccgggtcg cgcagggcga actcccgccc ccacggctgc tcgccgatct
     2101 cggtcatggc cggcccggag gcgtcccgga agttcgtgga cacgacctcc gaccactcgg
     2161 cgtacagctc gtccaggccg cgcacccaca cccaggccag ggtgttgtcc ggcaccacct
     2221 ggtcctggac cgcgctgatg aacagggtca cgtcgtcccg gaccacaccg gcgaagtcgt
     2281 cctccacgaa gtcccgggag aacccgagcc ggtcggtcca gaactcgacc gctccggcga
     2341 cgtcgcgcgc ggtgagcacc ggaacggcac tggtcaactt ggccatgatg gctcctcctg
     2401 tcaggagagg aaagagaaga aggttagtac aattgctata gtgagttgta ttatactatg
     2461 cagatatact atgccaatga ttaattgtca aactagggct gcagggttca tagtgccact
     2521 tttcctgcac tgccccatct cctgcccacc ctttcccagg catagacagt cagtgactta
     2581 ccaaactcac aggagggaga aggcagaagc ttgagacaga cccgcgggac cgccgaactg
     2641 cgaggggacg tggctagggc ggcttctttt atggtgcgcc ggccctcgga ggcagggcgc
     2701 tcggggaggc ctagcggcca atctgcggtg gcaggaggcg gggccgaagg ccgtgcctga
     2761 ccaatccgga gcacatagga gtctcagccc cccgccccaa agcaagggga agtcacgcgc
     2821 ctgtagcgcc agcgtgttgt gaaatggggg cttggggggg ttggggccct gactagtcaa
     2881 aacaaactcc cattgacgtc aatggggtgg agacttggaa atccccgtga gtcaaaccgc
     2941 tatccacgcc cattgatgta ctgccaaaac cgcatcatca tggtaatagc gatgactaat
     3001 acgtagatgt actgccaagt aggaaagtcc cataaggtca tgtactgggc ataatgccag
     3061 gcgggccatt taccgtcatt gacgtcaata gggggcgtac ttggcatatg atacacttga
     3121 tgtactgcca agtgggcagt ttaccgtaaa tactccaccc attgacgtca atggaaagtc
     3181 cctattggcg ttactatggg aacatacgtc attattgacg tcaatgggcg ggggtcgttg
     3241 ggcggtcagc caggcgggcc atttaccgta agttatgtaa cgcctgcagg ttaattaaga
     3301 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     3361 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     3421 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     3481 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     3541 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     3601 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     3661 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     3721 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     3781 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     3841 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     3901 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     3961 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     4021 tcatggctag ttaattaaca tttaaatcag cggccggccg caataaaata tctttatttt
     4081 cattacatct gtgtgttggt tttttgtgtg aatcgtaact aacatacgct ctccatcaaa
     4141 acaaaacgaa acaaaacaaa ctagcaaaat aggctgtccc cagtgcaagt gcaggtgcca
     4201 gaacatttct ctat
//
