pFtsZ-SYFP2 vector (V006169)

LOCUS       40924_20641        9158 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pFtsZ-SYFP2, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9158)
  AUTHORS   Zhang JY, Lin GM, Xing WY, Zhang CC.
  TITLE     Diversity of Growth Patterns Probed in Live Cyanobacterial Cells 
            Using a Fluorescent Analog of a Peptidoglycan Precursor
  JOURNAL   Front Microbiol 9, 791 (2018)
  PUBMED    29740419
REFERENCE   2  (bases 1 to 9158)
  AUTHORS   Zhang J-Y., Lin G-M., Xing W-Y., Zhang C-C.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2018) Institute of Hydrobiology, Donghu Nanlu, 
            Wuhan, Hubei 430072, 086
REFERENCE   3  (bases 1 to 9158)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9158)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Microbiol 9, 791 (2018)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-MAR-2018) Institute of Hydrobiology, Donghu Nanlu, Wuhan, Hubei 
            430072, 086"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9158
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(476..1894)
                     /label=SacB
                     /note="secreted levansucrase that renders bacterial growth 
                     sensitive to sucrose"
     promoter        complement(1895..2340)
                     /label=sacB promoter
                     /note="sacB promoter and control region"
     promoter        2488..2590
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             2591..3247
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     misc_feature    3347..4471
                     /note="region for homologous recombination derived from
                     Anabaena ftsZ"
     misc_feature    4487..4513
                     /label=domain linker
                     /note="domain linker"
     CDS             4514..5227
                     /label=SYFP2
                     /note="monomeric optimized variant of EYFP, identical to
                     mVenus but with L68V (Kremers et al., 2006)"
     misc_feature    5228..5257
                     /label=domain linker
                     /note="domain linker"
     CDS             5264..5287
                     /label=Strep-Tag II
                     /note="peptide that binds Strep-Tactin(R), an engineered 
                     form
                     of streptavidin"
     CDS             5324..5347
                     /label=Strep-Tag II
                     /note="peptide that binds Strep-Tactin(R), an engineered 
                     form
                     of streptavidin"
     terminator      5358..5405
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             5646..6434
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     regulatory      6495..6513
                     /regulatory_class="terminator"
     misc_feature    6595..7619
                     /note="region for homologous recombination derived from
                     Anabaena ftsZ"
     rep_origin      8103..8691
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(8877..9017)
                     /label=bom
                     /note="basis of mobility region from pBR322"