LOCUS       40924_20641        9158 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pFtsZ-SYFP2, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9158)
  AUTHORS   Zhang JY, Lin GM, Xing WY, Zhang CC.
  TITLE     Diversity of Growth Patterns Probed in Live Cyanobacterial Cells 
            Using a Fluorescent Analog of a Peptidoglycan Precursor
  JOURNAL   Front Microbiol 9, 791 (2018)
  PUBMED    29740419
REFERENCE   2  (bases 1 to 9158)
  AUTHORS   Zhang J-Y., Lin G-M., Xing W-Y., Zhang C-C.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2018) Institute of Hydrobiology, Donghu Nanlu, 
            Wuhan, Hubei 430072, 086
REFERENCE   3  (bases 1 to 9158)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9158)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Microbiol 9, 791 (2018)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-MAR-2018) Institute of Hydrobiology, Donghu Nanlu, Wuhan, Hubei 
            430072, 086"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9158
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(476..1894)
                     /label=SacB
                     /note="secreted levansucrase that renders bacterial growth 
                     sensitive to sucrose"
     promoter        complement(1895..2340)
                     /label=sacB promoter
                     /note="sacB promoter and control region"
     promoter        2488..2590
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             2591..3247
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     misc_feature    3347..4471
                     /note="region for homologous recombination derived from
                     Anabaena ftsZ"
     misc_feature    4487..4513
                     /label=domain linker
                     /note="domain linker"
     CDS             4514..5227
                     /label=SYFP2
                     /note="monomeric optimized variant of EYFP, identical to
                     mVenus but with L68V (Kremers et al., 2006)"
     misc_feature    5228..5257
                     /label=domain linker
                     /note="domain linker"
     CDS             5264..5287
                     /label=Strep-Tag II
                     /note="peptide that binds Strep-Tactin(R), an engineered 
                     form
                     of streptavidin"
     CDS             5324..5347
                     /label=Strep-Tag II
                     /note="peptide that binds Strep-Tactin(R), an engineered 
                     form
                     of streptavidin"
     terminator      5358..5405
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             5646..6434
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     regulatory      6495..6513
                     /regulatory_class="terminator"
     misc_feature    6595..7619
                     /note="region for homologous recombination derived from
                     Anabaena ftsZ"
     rep_origin      8103..8691
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(8877..9017)
                     /label=bom
                     /note="basis of mobility region from pBR322"
ORIGIN
        1 gctggctgtt ttacgcgtat gacaggctcc ggaagacggt tgttgcgcac gtattcggtg
       61 aacgcactat ggcgacgctg gggcgtctta tgagcctgct gtcacccttt gacgtggtga
      121 tatggatgac ggatggctgg ccgctgtatg aatcccgcct gaagggaaag ctgcacgtaa
      181 tcagcaagcg atatacgcag cgaattgagc ggcataacct gaatctgagg cagcacctgg
      241 cacggctggg acggaagtcg ctgtcgttct caaaatcggt ggagctgcat gacaaagtca
      301 tcgggcatta tctgaacata aaacactatc aataagttgg agtcattacc aaaaggttag
      361 gaatacggtt agccatttgc ctgcttttat atagttcata tgggattcac ctttatgttg
      421 ataagaaata aaagaaaatg ccaataggat atcggcattt tcttttgcgt ttttatttgt
      481 taactgttaa ttgtccttgt tcaaggatgc tgtctttgac aacagatgtt ttcttgcctt
      541 tgatgttcag caggaagctt ggcgcaaacg ttgattgttt gtctgcgtag aatcctctgt
      601 ttgtcatata gcttgtaatc acgacattgt ttcctttcgc ttgaggtaca gcgaagtgtg
      661 agtaagtaaa ggttacatcg ttaggatcaa gatccatttt taacacaagg ccagttttgt
      721 tcagcggctt gtatgggcca gttaaagaat tagaaacata accaagcatg taaatatcgt
      781 tagacgtaat gccgtcaatc gtcatttttg atccgcggga gtcagtgaac aggtaccatt
      841 tgccgttcat tttaaagacg ttcgcgcgtt caatttcatc tgttactgtg ttagatgcaa
      901 tcagcggttt catcactttt ttcagtgtgt aatcatcgtt tagctcaatc ataccgagag
      961 cgccgtttgc taactcagcc gtgcgttttt tatcgctttg cagaagtttt tgactttctt
     1021 gacggaagaa tgatgtgctt ttgccatagt atgctttgtt aaataaagat tcttcgcctt
     1081 ggtagccatc ttcagttcca gtgtttgctt caaatactaa gtatttgtgg cctttatctt
     1141 ctacgtagtg aggatctctc agcgtatggt tgtcgcctga gctgtagttg ccttcatcga
     1201 tgaactgctg tacattttga tacgtttttc cgtcaccgtc aaagattgat ttataatcct
     1261 ctacaccgtt gatgttcaaa gagctgtctg atgctgatac gttaacttgt gcagttgtca
     1321 gtgtttgttt gccgtaatgt ttaccggaga aatcagtgta gaataaacgg atttttccgt
     1381 cagatgtaaa tgtggctgaa cctgaccatt cttgtgtttg gtcttttagg atagaatcat
     1441 ttgcatcgaa tttgtcgctg tctttaaaga cgcggccagc gtttttccag ctgtcaatag
     1501 aagtttcgcc gactttttga tagaacatgt aaatcgatgt gtcatccgca tttttaggat
     1561 ctccggctaa tgcaaagacg atgtggtagc cgtgatagtt tgcgacagtg ccgtcagcgt
     1621 tttgtaatgg ccagctgtcc caaacctcca ggccttttgc agaagagata tttttaattg
     1681 tggacgaatc gaattcagga acttgatatt tttcattttt ttgctgttca gggatttgca
     1741 gcatatcatg gcgtgtaata tgggaaatgc cgtatgtttc cttatatggc ttttggttcg
     1801 tttctttcgc aaacgcttga gttgcgcctc ctgccagcag tgcggtagta aaggttaata
     1861 ctgttgcttg ttttgcaaac tttttgatgt tcatcgttca tgtctccttt tttatgtact
     1921 gtgttagcgg tctgcttctt ccagccctcc tgtttgaaga tggcaagtta gttacgcaca
     1981 ataaaaaaag acctaaaata tgtaaggggt gacgccaaag tatacacttt gccctttaca
     2041 cattttaggt cttgcctgct ttatcagtaa caaacccgcg cgatttactt ttcgacctca
     2101 ttctattaga ctctcgtttg gattgcaact ggtctatttt cctcttttgt ttgatagaaa
     2161 atcataaaag gatttgcaga ctacgggcct aaagaactaa aaaatctatc tgtttctttt
     2221 cattctctgt attttttata gtttctgttg catgggcata aagttgcctt tttaatcaca
     2281 attcagaaaa tatcataata tctcatttca ctaaataata gtgaacggca ggtatatgtg
     2341 atgggttaaa aaggatcgat cctctagcta gagtcgactc atcgaataaa tacctgtgac
     2401 ggaagatcac ttcgcagaat aaataaatcc tggtgtccct gttgataccg ggaagccctg
     2461 ggccaacttt tggcgaaaat gagacgttga tcggcacgta agaggttcca actttcacca
     2521 taatgaaata agatcactac cgggcgtatt ttttgagtta tcgagatttt caggagctaa
     2581 ggaagctaaa atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca
     2641 tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt
     2701 tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc
     2761 ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggaattcc gtatggcaat
     2821 gaaagacggt gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga
     2881 gcaaactgaa acgttttcat cgctctggag tgaataccac gacgatttcc ggcagtttct
     2941 acacatatat tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg
     3001 gtttattgag aatatgtttt tcgtctcagc caatccctgg gtgagtttca ccagttttga
     3061 tttaaacgtg gccaatatgg acaacttctt cgcccccgtt ttcaccatgg gcaaatatta
     3121 tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga
     3181 tggcttccat gtcggcagaa tgcttaatga attacaacag tactgcgatg agtggcaggg
     3241 cggggcgtaa tttttttaag gcagttattg gtgcccttaa acgcctggtg ctacgcctga
     3301 ataagtgata ataagcggat gaatggcaga aattcgatat ctagatcgta ttggcgagat
     3361 tgttcctggg cgcgttgcca atatcaaagt aattggtgtt ggcggtggtg gtggtaatgc
     3421 tgttaaccgc atgattgaat ctgatgtctc tggtgtagag ttttggtcaa ttaatactga
     3481 tgctcaagct ttgaccttgg caggcgctcc tagtcgttta caaatcgggc agaaactcac
     3541 gaggggttta ggtgcaggtg gtaatcctgc aattggtcaa aaagcagctg aagaatcacg
     3601 cgatgaaatt gctacagcct tagaaggtgc tgatttagta tttatcactg ctgggatggg
     3661 aggtggtact ggtactggtg ctgcaccaat tgttgcggaa gtggcaaagg aaatgggcgc
     3721 tcttactgtt ggagtggtaa cacgtccttt tgtttttgaa ggtcgtcgcc ggactagtca
     3781 ggcagaacaa ggaattgaag ggctaaaaag tagagttgat actttaatta ttattcctaa
     3841 caataaatta ttggaagtga ttccagaaca aacaccagtc caagaagcgt ttcgttatgc
     3901 agatgacgta ctacgtcaag gggtacaagg catttccgac atcattacta ttcctggttt
     3961 ggtaaatgta gactttgctg acgtgcgggc tgtcatggcc gatgcgggat cagcattgat
     4021 gggtattggt gtgagttcag gaaaatctag agccagagaa gctgcgatcg ccgctatatc
     4081 ttcaccactg ctagaatgtt ctatcgaagg tgctagaggt gttgttttta acatcactgg
     4141 tggtagtgac ctcactctac atgaagtaaa tgctgccgcc gaaacgattt atgaagtagt
     4201 tgatcccaac gccaacatta tttttggtgc cgtgattgat gataggttgc aaggagaggt
     4261 gcgaattacc gtcattgcta ctggatttac tggggaaata caagctgctc cccaacaaaa
     4321 cgctgctaat gcaagagttg tatctgctcc cccaaaacgg acacccacac aaacaccact
     4381 aactaattct cctgcaccta ctcctgaacc aaaagagaaa tctggattgg atatccctga
     4441 ttttcttcag agacggcgac cacccaaaaa tggatccaag gcctctggtg gatctggagg
     4501 tagtggtggt agtgttagca aaggtgaaga attgtttaca ggtgtagtac ctatcttagt
     4561 tgaattagac ggtgatgtta acggtcataa atttagcgtc agcggtgaag gtgaaggtga
     4621 cgcaacttac ggcaaattga ccttgaagtt gatttgcaca acaggtaagt taccagtccc
     4681 ttggcctacc ttggtcacaa ccttgggtta tggcgttcaa tgtttcgctc gttatccaga
     4741 ccacatgaaa caacacgatt tcttcaaaag cgcaatgcca gaaggttatg tacaagaacg
     4801 taccatcttt ttcaaagacg acggtaacta caaaacacgt gctgaagtca aattcgaagg
     4861 cgacaccttg gttaatcgca ttgaactgaa gggtattgat ttcaaagaag atggcaatat
     4921 cttgggtcac aaattggaat acaactacaa ctcccacaac gtttacatca ccgcagataa
     4981 acaaaaaaat ggtatcaaag ctaatttcaa aatccgtcac aacattgaag atggtggtgt
     5041 tcaattagct gatcattacc aacaaaacac ccctattggt gacggtccag tcctgttgcc
     5101 agataaccat tatttgtctt accaaagcaa actgagcaaa gatccaaacg aaaaacgcga
     5161 ccacatggtg ttgttggaat ttgtaaccgc tgctggtatt accttgggta tggatgaatt
     5221 gtataaaggt tctggtggtg gtagcactag cgtcggtagc gcttggagcc atccccaatt
     5281 tgaaaaaggt ggtggcagcg gcggtggtag cggtggtagc gcttggtctc acccacaatt
     5341 cgaaaagtaa gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt
     5401 ttttgctgaa gatgcacctt gccgtagaag gctcacgcaa ctggtccaga accttgaccg
     5461 aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttg
     5521 gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt
     5581 tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttaa
     5641 acatcatgag ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg
     5701 tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg
     5761 atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc gtaaggcttg
     5821 atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag
     5881 agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt
     5941 ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg
     6001 caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa
     6061 gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg
     6121 aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact
     6181 gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa
     6241 ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc
     6301 agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct
     6361 tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc gagatcacca
     6421 aggtagtcgg caaataatgt ctcccgtgga tgaccttttg aatgaccttt aatagattat
     6481 attactaatt aattggggac cctagaggtc ccctttttta ttttaaaaat tttttcacaa
     6541 aacggtttac aagcataaag cttgctcaat caatcaccgg atcatcagta ctccctgcta
     6601 attttcaagt tcagaggtat gttttttacc tcaggtaaat cttgttgctg ttagaaataa
     6661 attgctacta ttcaaaaaca gaatacactt gggcgatagt ttattgactt ttacgcctaa
     6721 ttgtgtattt gtgatacaca ggagtgagac cgaaaagaga aatcttcacg tcctcctcct
     6781 gtagttttcc tttgattacc tgtgtttaat tttgaatttg taggtttttt tcaacccatt
     6841 gaattacttg atgagccagg cgagtaccat ctagtcggtc gatttccctt atcccagttg
     6901 ggctagtgac gttaacttct gtgagatagc cacctatcac atcaataccc acaaagatta
     6961 acccatcttg gcgtaaattt gcggcgattt ggctacaaat ttcttcttct cgtggggtaa
     7021 tttcggtttt tgcaaccgtc ccaccagtgg ccatgttatt acgaaaatca ctgccacttg
     7081 ccaaacgatt gagagcgcca atgggttcac cattaagtaa aatgatccgc ttatctccct
     7141 ctttggcctc tggtagataa gtttgtacca ttacgggtaa tcggccttgc tgggtgctga
     7201 gttcgacgat ggagttaaaa ttgcgatcgc ctgcttgtag aaatagaatc ccttccccag
     7261 ctttgttacc caagggtttg agaacagtag cgccttttgc ttcgacaaat tgccggataa
     7321 aatctttatc tgcactgacg atggtttcgg gaattgcctt ggtaaattga agggcataca
     7381 ttttttcatt tgccccacgg ataccattgg gattgttaat taccaaagtt ttacgttgat
     7441 caacgtaatc tagaacatag gtagcgtaaa gataggcatc gttcacgggc ggatctgtcc
     7501 gcatgaatac agcatccatc gtctccaaag aactgaagga acgagtattt aatgtatacc
     7561 aaggacttgc tgccacccat cgtccatcta ttagctgcac tggtacaagt tctacctgca
     7621 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     7681 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     7741 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     7801 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     7861 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     7921 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     7981 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     8041 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     8101 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     8161 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     8221 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
     8281 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     8341 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     8401 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     8461 acctacaccg aactgagata cctacagcgt gagcattgag aaagcgccac gcttcccgaa
     8521 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     8581 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     8641 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     8701 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     8761 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     8821 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg
     8881 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc
     8941 agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg
     9001 actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     9061 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     9121 agaggttttc accgtcatca ccgaaacgcg cgaggcag
//
