pmGFP-ADAR1-p150 vector (V007226)

Basic Vector Information

      • Vector Name:
      • pmGFP-ADAR1-p150
      • Antibiotic Resistance:
      • Ampicillin
      • Length:
      • 9801 bp
      • Type:
      • Mammalian Expression
      • Selection Marker:
      • Neomycin (select with G418)
      • Promoter:
      • CMV
      • Cloning Method:
      • Restriction Enzyme
      • 5' Primer:
      • AAAGGGaagcttATGAATCCGCGGCAGGGGTATTCC
      • 3' Primer:
      • AAAGGGtctagaCTATACTGGGCAGAGATAAAAGTTC

pmGFP-ADAR1-p150 vector Vector Map

pmGFP-ADAR1-p1509801 bp4008001200160020002400280032003600400044004800520056006000640068007200760080008400880092009600CMV enhancerCMV promoterT7 promoterMycEGFPADARbGH poly(A) signalf1 oriSV40 promoterNeoR/KanRSV40 poly(A) signalM13 revlac operatorlac promoterCAP binding siteL4440oriAmpRAmpR promoterpRS-marker

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pmGFP-ADAR1-p150 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       pmGFP-ADAR1-p150        9801 bp DNA     circular SYN 09-DEC-2021
DEFINITION  ADAR1-p150 expression plasmid..
ACCESSION   .
VERSION     .
KEYWORDS    pmGFP-ADAR1-p150.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9801)
  AUTHORS   Galipon J, Ishii R, Suzuki Y, Tomita M, Ui-Tei K
  TITLE     Differential Binding of Three Major Human ADAR Isoforms to Coding 
            and Long Non-Coding Transcripts.
  JOURNAL   Genes (Basel). 2017 Feb 11;8(2). pii: genes8020068. doi: 
            10.3390/genes8020068.
  PUBMED    28208661
REFERENCE   2  (bases 1 to 9801)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 9801)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes
            (Basel)."; date: "2017-02-11"; pages: " 10.3390/genes8020068"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9801
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        155..534
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        535..738
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        783..801
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             829..858
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             868..1584
                     /label=EGFP
                     /note="enhanced GFP"
     CDS             1600..5277
                     /gene="ADAR"
                     /label=ADAR
                     /note="Double-stranded RNA-specific adenosine deaminase
                     from Homo sapiens. Accession#: P55265"
     polyA_signal    5318..5542
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5588..6016
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        6030..6359
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             6426..7217
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    7394..7527
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7564..7580)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(7588..7604)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7612..7642)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7657..7678)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     primer_bind     complement(7795..7812)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7966..8554)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8728..9585)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(9586..9690)
                     /label=AmpR promoter
     primer_bind     complement(9765..9784)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable
                     marker"

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