Basic Vector Information
pmGFP-ADAR1-p150 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pmGFP-ADAR1-p150 vector Sequence
LOCUS pmGFP-ADAR1-p150 9801 bp DNA circular SYN 09-DEC-2021 DEFINITION ADAR1-p150 expression plasmid.. ACCESSION . VERSION . KEYWORDS pmGFP-ADAR1-p150. SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9801) AUTHORS Galipon J, Ishii R, Suzuki Y, Tomita M, Ui-Tei K TITLE Differential Binding of Three Major Human ADAR Isoforms to Coding and Long Non-Coding Transcripts. JOURNAL Genes (Basel). 2017 Feb 11;8(2). pii: genes8020068. doi: 10.3390/genes8020068. PUBMED 28208661 REFERENCE 2 (bases 1 to 9801) TITLE Direct Submission REFERENCE 3 (bases 1 to 9801) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Genes (Basel)."; date: "2017-02-11"; pages: " 10.3390/genes8020068" COMMENT SGRef: number: 2; type: "Journal Article" FEATURES Location/Qualifiers source 1..9801 /mol_type="other DNA" /organism="synthetic DNA construct" enhancer 155..534 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 535..738 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" promoter 783..801 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 829..858 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" CDS 868..1584 /label=EGFP /note="enhanced GFP" CDS 1600..5277 /gene="ADAR" /label=ADAR /note="Double-stranded RNA-specific adenosine deaminase from Homo sapiens. Accession#: P55265" polyA_signal 5318..5542 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 5588..6016 /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 6030..6359 /label=SV40 promoter /note="SV40 enhancer and early promoter" CDS 6426..7217 /label=NeoR/KanR /note="aminoglycoside phosphotransferase" polyA_signal 7394..7527 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(7564..7580) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind complement(7588..7604) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(7612..7642) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(7657..7678) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(7795..7812) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(7966..8554) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(8728..9585) /label=AmpR /note="beta-lactamase" promoter complement(9586..9690) /label=AmpR promoter primer_bind complement(9765..9784) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker"
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