LOCUS       pmGFP-ADAR1-p150        9801 bp DNA     circular SYN 09-DEC-2021
DEFINITION  ADAR1-p150 expression plasmid..
ACCESSION   .
VERSION     .
KEYWORDS    pmGFP-ADAR1-p150.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9801)
  AUTHORS   Galipon J, Ishii R, Suzuki Y, Tomita M, Ui-Tei K
  TITLE     Differential Binding of Three Major Human ADAR Isoforms to Coding 
            and Long Non-Coding Transcripts.
  JOURNAL   Genes (Basel). 2017 Feb 11;8(2). pii: genes8020068. doi: 
            10.3390/genes8020068.
  PUBMED    28208661
REFERENCE   2  (bases 1 to 9801)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 9801)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes
            (Basel)."; date: "2017-02-11"; pages: " 10.3390/genes8020068"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9801
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        155..534
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        535..738
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        783..801
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             829..858
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             868..1584
                     /label=EGFP
                     /note="enhanced GFP"
     CDS             1600..5277
                     /gene="ADAR"
                     /label=ADAR
                     /note="Double-stranded RNA-specific adenosine deaminase
                     from Homo sapiens. Accession#: P55265"
     polyA_signal    5318..5542
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5588..6016
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        6030..6359
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             6426..7217
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    7394..7527
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7564..7580)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(7588..7604)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7612..7642)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7657..7678)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     primer_bind     complement(7795..7812)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7966..8554)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8728..9585)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(9586..9690)
                     /label=AmpR promoter
     primer_bind     complement(9765..9784)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable
                     marker"
ORIGIN
        1 ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca
       61 acaaggcaag gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg
      121 ctgcttcgcg atgtacgggc cagatatacg cgttgacatt gattattgac tagttattaa
      181 tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg cgttacataa
      241 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata
      301 atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag
      361 tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc
      421 cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta
      481 tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg
      541 cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt
      601 ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca
      661 aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag
      721 gtctatataa gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa
      781 attaatacga ctcactatag ggagacccaa gctggctagc ccaccatgga acaaaaactc
      841 atctcagaag aggatctgtc cggcggcatg gtgagcaagg gcgaggagct gttcaccggg
      901 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
      961 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
     1021 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc
     1081 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
     1141 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
     1201 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     1261 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
     1321 tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac
     1381 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
     1441 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac
     1501 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
     1561 ctcggcatgg acgagctgta caagtccggc ggcaagctta tgaatccgcg gcaggggtat
     1621 tccctcagcg gatactacac ccatccattt caaggctatg agcacagaca gctcagatac
     1681 cagcagcctg ggccaggatc ttcccccagt agtttcctgc ttaagcaaat agaatttctc
     1741 aaggggcagc tcccagaagc accggtgatt ggaaagcaga caccgtcact gccaccttcc
     1801 ctcccaggac tccggccaag gtttccagta ctacttgcct ccagtaccag aggcaggcaa
     1861 gtggacatca ggggtgtccc caggggcgtg catctcggaa gtcaggggct ccagagaggg
     1921 ttccagcatc cttcaccacg tggcaggagt ctgccacaga gaggtgttga ttgcctttcc
     1981 tcacatttcc aggaactgag tatctaccaa gatcaggaac aaaggatctt aaagttcctg
     2041 gaagagcttg gggaagggaa ggccaccaca gcacatgatc tgtctgggaa acttgggact
     2101 ccgaagaaag aaatcaatcg agttttatac tccctggcaa agaagggcaa gctacagaaa
     2161 gaggcaggaa cacccccttt gtggaaaatc gcggtctcca ctcaggcttg gaaccagcac
     2221 agcggagtgg taagaccaga cggtcatagc caaggagccc caaactcaga cccgagtttg
     2281 gaaccggaag acagaaactc cacatctgtc tcagaagatc ttcttgagcc ttttattgca
     2341 gtctcagctc aggcttggaa ccagcacagc ggagtggtaa gaccagacag tcatagccaa
     2401 ggatccccaa actcagaccc aggtttggaa cctgaagaca gcaactccac atctgccttg
     2461 gaagatcctc ttgagttttt agacatggcc gagatcaagg agaaaatctg cgactatctc
     2521 ttcaatgtgt ctgactcctc tgccctgaat ttggctaaaa atattggcct taccaaggcc
     2581 cgagatataa atgctgtgct aattgacatg gaaaggcagg gggatgtcta tagacaaggg
     2641 acaacccctc ccatatggca tttgacagac aagaagcgag agaggatgca aatcaagaga
     2701 aatacgaaca gtgttcctga aaccgctcca gctgcaatcc ctgagaccaa aagaaacgca
     2761 gagttcctca cctgtaatat acccacatca aatgcctcaa ataacatggt aaccacagaa
     2821 aaagtggaga atgggcagga acctgtcata aagttagaaa acaggcaaga ggccagacca
     2881 gaaccagcaa gactgaaacc acctgttcat tacaatggcc cctcaaaagc agggtatgtt
     2941 gactttgaaa atggccagtg ggccacagat gacatcccag atgacttgaa tagtatccgc
     3001 gcagcaccag gtgagtttcg agccatcatg gagatgccct ccttctacag tcatggcttg
     3061 ccacggtgtt caccctacaa gaaactgaca gagtgccagc tgaagaaccc catcagcggg
     3121 ctgttagaat atgcccagtt cgctagtcaa acctgtgagt tcaacatgat agagcagagt
     3181 ggaccacccc atgaacctcg atttaaattc caggttgtca tcaatggccg agagtttccc
     3241 ccagctgaag ctggaagcaa gaaagtggcc aagcaggatg cagctatgaa agccatgaca
     3301 attctgctag aggaagccaa agccaaggac agtggaaaat cagaagaatc atcccactat
     3361 tccacagaga aagaatcaga gaagactgca gagtcccaga cccccacccc ttcagccaca
     3421 tccttctttt ctgggaagag ccccgtcacc acactgcttg agtgtatgca caaattgggg
     3481 aactcctgcg aattccgtct cctgtccaaa gaaggccctg cccatgaacc caagttccaa
     3541 tactgtgttg cagtgggagc ccaaactttc cccagtgtga gtgctcccag caagaaagtg
     3601 gcaaagcaga tggccgcaga ggaagccatg aaggccctgc atggggaggc gaccaactcc
     3661 atggcttctg ataaccagcc tgaaggtatg atctcagagt cacttgataa cttggaatcc
     3721 atgatgccca acaaggtcag gaagattggc gagctcgtga gatacctgaa caccaaccct
     3781 gtgggtggcc ttttggagta cgcccgctcc catggctttg ctgctgaatt caagttggtc
     3841 gaccagtccg gacctcctca cgagcccaag ttcgtttacc aagcaaaagt tgggggtcgc
     3901 tggttcccag ccgtctgcgc acacagcaag aagcaaggca agcaggaagc agcagatgcg
     3961 gctctccgtg tcttgattgg ggagaacgag aaggcagaac gcatgggttt cacagaggta
     4021 accccagtga caggggccag tctcagaaga actatgctcc tcctctcaag gtccccagaa
     4081 gcacagccaa agacactccc tctcactggc agcaccttcc atgaccagat agccatgctg
     4141 agccaccggt gcttcaacac tctgactaac agcttccagc cctccttgct cggccgcaag
     4201 attctggccg ccatcattat gaaaaaagac tctgaggaca tgggtgtcgt cgtcagcttg
     4261 ggaacaggga atcgctgtgt aaaaggagat tctctcagcc taaaaggaga aactgtcaat
     4321 gactgccatg cagaaataat ctcccggaga ggcttcatca ggtttctcta cagtgagtta
     4381 atgaaataca actcccagac tgcgaaggat agtatatttg aacctgctaa gggaggagaa
     4441 aagctccaaa taaaaaagac tgtgtcattc catctgtata tcagcactgc tccgtgtgga
     4501 gatggcgccc tctttgacaa gtcctgcagc gaccgtgcta tggaaagcac agaatcccgc
     4561 cactaccctg tcttcgagaa tcccaaacaa ggaaagctcc gcaccaaggt ggagaacgga
     4621 gaaggcacaa tccctgtgga atccagtgac attgtgccta cgtgggatgg cattcggctc
     4681 ggggagagac tccgtaccat gtcctgtagt gacaaaatcc tacgctggaa cgtgctgggc
     4741 ctgcaagggg cactgttgac ccacttcctg cagcccattt atctcaaatc tgtcacattg
     4801 ggttaccttt tcagccaagg gcatctgacc cgtgctattt gctgtcgtgt gacaagagat
     4861 gggagtgcat ttgaggatgg actacgacat ccctttattg tcaaccaccc caaggttggc
     4921 agagtcagca tatatgattc caaaaggcaa tccgggaaga ctaaggagac aagcgtcaac
     4981 tggtgtctgg ctgatggcta tgacctggag atcctggacg gtaccagagg cactgtggat
     5041 gggccacgga atgaattgtc ccgggtctcc aaaaagaaca tttttcttct atttaagaag
     5101 ctctgctcct tccgttaccg cagggatcta ctgagactct cctatggtga ggccaagaaa
     5161 gctgcccgtg actacgagac ggccaagaac tacttcaaaa aaggcctgaa ggatatgggc
     5221 tatgggaact ggattagcaa accccaggag gaaaagaact tttatctctg cccagtatag
     5281 tctagagggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca
     5341 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     5401 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     5461 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
     5521 ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg
     5581 tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     5641 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     5701 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     5761 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     5821 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     5881 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     5941 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     6001 aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
     6061 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
     6121 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     6181 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     6241 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg
     6301 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
     6361 agctcccggg agcttgtata tccattttcg gatctgatca agagacagga tgaggatcgt
     6421 ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc
     6481 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc
     6541 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg
     6601 aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag
     6661 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg
     6721 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg
     6781 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac
     6841 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg
     6901 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc
     6961 ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg
     7021 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc
     7081 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc
     7141 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc
     7201 ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc
     7261 caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
     7321 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
     7381 cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     7441 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     7501 catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc
     7561 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     7621 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     7681 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     7741 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     7801 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     7861 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     7921 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     7981 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     8041 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     8101 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     8161 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     8221 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     8281 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     8341 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     8401 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     8461 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     8521 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     8581 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     8641 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     8701 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     8761 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     8821 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     8881 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     8941 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     9001 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     9061 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     9121 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     9181 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     9241 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     9301 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     9361 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     9421 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     9481 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     9541 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     9601 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     9661 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     9721 cgacggatcg ggagatctcc cgatccccta tggtgcactc tcagtacaat ctgctctgat
     9781 gccgcatagt taagccagta t
//
