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pMAD vector (V007385)

Basic Vector Information

Vector Name:
pMAD
Antibiotic Resistance:
Ampicillin, Erythromycin
Length:
9666 bp
Type:
Prokaryotic Expression Vectors
Replication origin:
ori

pMAD vector Vector Map

pMAD9666 bp4008001200160020002400280032003600400044004800520056006000640068007200760080008400880092009600ermCprebgaBropbomoriAmpRAmpR promoter

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pMAD vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_29656        9666 bp DNA     circular SYN 13-JAN-2022
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9666)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 9666)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9666
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(98..829)
                     /gene="ermC"
                     /label=ermC
                     /note="rRNA adenine N-6-methyltransferase from
                     Staphylococcus aureus. Accession#: P02979"
     CDS             1241..2449
                     /gene="pre"
                     /label=pre
                     /note="Plasmid recombination enzyme type 1 from
                     Staphylococcus aureus. Accession#: P03857"
     CDS             complement(3973..5988)
                     /gene="bgaB"
                     /label=bgaB
                     /note="Beta-galactosidase bgaB from Geobacillus
                     kaustophilus. Accession#: P19668"
     CDS             7223..7411
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     misc_feature    7516..7656
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(7842..8430)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8604..9461)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(9462..9566)
                     /label=AmpR promoter

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