LOCUS       Exported                9664 bp DNA     circular SYN 04-DEC-2025
DEFINITION  Exported.
ACCESSION   V007385
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9664)
  AUTHORS   caoheibi
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 9664)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 9664)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9664)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 9664)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     SGRef: number: 2; type: "Journal Article"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9664
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(98..829)
                     /gene="ermC"
                     /label=rRNA adenine N-6-methyltransferase
                     /note="rRNA adenine N-6-methyltransferase from
                     Staphylococcus aureus. Accession#: P02979"
     rep_origin      1241..3748
                     /label=pE194ts
                     /note="temperature-sensitive replication origin (pE194ts) 
                     derived from the Staphylococcus aureus plasmid pE194, which
                     enables replication at permissive temperatures (¡Ü32(o)C) 
                     but is fully blocked at nonpermissive temperatures 
                     (¡Ý37(o)C)"
     CDS             1241..2449
                     /codon_start=1
                     /gene="pre"
                     /label=MobV family relaxase
                     /label=Plasmid recombination enzyme type 1
                     /note="Plasmid recombination enzyme type 1 from
                     Staphylococcus aureus. Accession#: P03857"
                     /translation="MSHSILRVARVKGSSNTNGIQRHNQRENKNYNNKDINHEETYKNY
                     DLINAQNIKYKDKIDETIDENYSGKRKIRSDAIRHVDGLVTSDKDFFDDLSGEEIERFF
                     KDSLEFLENEYGKENMLYATVHLDERVPHMHFGFVPLTEDGRLSAKEQLGNKKDFTQLQ
                     DRFNEYVNEKGYELERGTSKEVTEREHKAMDQYKKDTVFHKQELQEVKDELQKANKQLQ
                     SGIEHMRSTKPFDYENERTGLFSGREETGRKILTADEFERLQETISSAERIVDDYENIK
                     STDYYTENQELKKRRESLKEVVNTWKEGYHEKSKEVNKLKRENDSLNEQLNVSEKFQDS
                     TVTLYRAARANFPGFEKGFNRLKEKFFNDSKFERVGQFMDVVQDNVQKVDRKREKQRTD
                     DLEM"
     CDS             complement(3974..5989)
                     /gene="bgaB"
                     /label=Beta-galactosidase bgaB
                     /note="Beta-galactosidase bgaB from Geobacillus
                     kaustophilus. Accession#: P19668"
     promoter        complement(6066..6191)
                     /label=clpB
                     /note="clpB promoter from Staphylococcus aureus"
     CDS             7221..7409
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     misc_feature    7514..7654
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(7840..8428)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8602..9459)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(9460..9564)
                     /label=AmpR promoter
ORIGIN
        1 gaattaattc tcatgtttga cagcttatca tcgattcaca aaaaataggc acacgaaaaa
       61 caagttaagg gatgcagttt atgcatccct taacttactt attaaataat ttatagctat
      121 tgaaaagaga taagaattgt tcaaagctaa tattgtttaa atcgtcaatt cctgcatgtt
      181 ttaaggaatt gttaaattga ttttttgtaa atattttctt gtattctttg ttaacccatt
      241 tcataacgaa ataattatac ttttgtttat ctttgtgtga tattcttgat ttttttctac
      301 ttaatctgat aagtgagcta ttcactttag gtttaggatg aaaatattct cttggaacca
      361 tacttaatat agaaatatca acttctgcca ttaaaagtaa tgccaatgag cgttttgtat
      421 ttaataatct tttagcaaac ccgtattcca cgattaaata aatctcatta gctatactat
      481 caaaaacaat tttgcgtatt atatccgtac ttatgttata aggtatatta ccatatattt
      541 tataggattg gtttttagga aatttaaact gcaatatatc cttgtttaaa acttggaaat
      601 tatcgtgatc aacaagttta ttttctgtag ttttgcataa tttatggtct atttcaatgg
      661 cagttacgaa attacacctc tttactaatt caagggtaaa atggcctttt cctgagccga
      721 tttcaaagat attatcatgt tcatttaatc ttatatttgt cattatttta tctatattat
      781 gttttgaagt aataaagttt tgactgtgtt ttatattttt ctcgttcatt ataaccctct
      841 ttaatttggt tatatgaatt ttgcttatta acgattcatt ataaccactt attttttgtt
      901 tggttgataa tgaactgtgc tgattacaaa aatactaaaa atgcccatat tttttcctcc
      961 ttataaaatt agtataatta tagcacgagc tctgataaat atgaacatga tgagtgatcg
     1021 ttaaatttat actgcaatcg gatgcgatta ttgaataaaa gatatgagag atttatctaa
     1081 tttctttttt cttgtaaaaa aagaaagttc ttaaaggttt tatagttttg gtcgtagagc
     1141 acacggttta acgacttaat tacgaagtaa ataagtctag tgtgttagac tttatgaaat
     1201 ctatatacgt ttatatatat ttattatccg gaggtgtagc atgtctcatt caattttgag
     1261 ggttgccaga gttaaaggat caagtaatac aaacgggata caaagacata atcaaagaga
     1321 gaataaaaac tataataata aagacataaa tcatgaggaa acatataaaa attatgattt
     1381 gattaacgca caaaatataa agtataaaga taaaattgat gaaacgattg atgagaatta
     1441 ttcagggaaa cgtaaaattc ggtcagatgc aattcgacat gtggacggac tggttacaag
     1501 tgataaagat ttctttgatg atttaagcgg agaagaaata gaacgatttt ttaaagatag
     1561 cttggagttt ctagaaaatg aatacggtaa ggaaaatatg ctgtatgcga ctgtccatct
     1621 ggatgaaaga gtcccacata tgcactttgg ttttgtccct ttaacagagg acgggagatt
     1681 gtctgcaaaa gaacagttag gcaacaagaa agactttact caattacaag atagatttaa
     1741 tgagtatgtg aatgagaaag gttatgaact tgaaagaggc acgtccaaag aggttacaga
     1801 acgagaacat aaagcgatgg atcagtacaa gaaagatact gtatttcata aacaggaact
     1861 gcaagaagtt aaggatgagt tacagaaggc aaataagcag ttacagagtg gaatagagca
     1921 tatgaggtct acgaaaccct ttgattatga aaatgagcgt acaggtttgt tctctggacg
     1981 tgaagagact ggtagaaaga tattaactgc tgatgaattt gaacgcctgc aagaaacaat
     2041 ctcttctgca gaacggattg ttgatgatta cgaaaatatt aagagcacag actattacac
     2101 agaaaatcaa gaattaaaaa aacgtagaga gagtttgaaa gaagtagtga atacatggaa
     2161 agaggggtat cacgaaaaaa gtaaagaggt taataaatta aagcgagaga atgatagttt
     2221 gaatgagcag ttgaatgtat cagagaaatt tcaagatagt acagtgactt tatatcgtgc
     2281 tgcgagggcg aatttccctg ggtttgagaa agggtttaat aggcttaaag agaaattctt
     2341 taatgattcc aaattcgagc gtgtgggaca gtttatggat gttgtacagg ataatgtcca
     2401 gaaggtcgat agaaagcgtg agaaacagcg tacagacgat ttagagatgt agaggtactt
     2461 ttatgccgag aaaacttttt gcgtgtgaca gtccttaaaa tatacttaga gcgtaagcga
     2521 aagtagtagc gacagctatt aactttcggt tgcaaagctc taggattttt aatggacgca
     2581 gcgcatcaca cgcaaaaagg aaattggaat aaatgcgaaa tttgagatgt taattaaaga
     2641 cctttttgag gtcttttttt cttagatttt tggggttatt taggggagaa aacatagggg
     2701 ggtactacga cctcccccct aggtgtccat tgtccattgt ccaaacaaat aaataaatat
     2761 tgggttttta atgttaaaag gttgtttttt atgttaaagt gaaaaaaaca gatgttggga
     2821 ggtacagtga tggttgtaga tagaaaagaa gagaaaaaag ttgctgttac tttaagactt
     2881 acaacagaag aaaatgagat attaaataga atcaaagaaa aatataatat tagcaaatca
     2941 gatgcaaccg gtattctaat aaaaaaatat gcaaaggagg aatacggtgc attttaaaca
     3001 aaaaaagata gacagcactg gcatgctgcc tatctatgac taaattttgt taaatgtatt
     3061 agcaccgtta ttatatcatg agcgaaaatg taataaaaga aactgaaaac aagaaaaatt
     3121 caagaggacg taattggaca tttgttttat atccagaatc agcaaaagcc gagtggttag
     3181 agtatttaaa agagttacac attcaatttg tagtgtctcc attacatgat agggatactg
     3241 atacagaaga taggatgaaa aaagagcatt atcatattct agtgatgtat gagggtaata
     3301 aatcttatga acagataaaa ataattacag aagaattgaa tgcgactatt ccgcagattg
     3361 caggaagtgt gaaaggtctt gtgagatata tgcttcacat ggacgatcct aataaattta
     3421 aatatcaaaa agaagatatg atagtttatg gcggtgtaga tgttgatgaa ttattaaaga
     3481 aaacaacaac agatagatat aaattaatta aagaaatgat tgagtttatt gatgaacaag
     3541 gaatcgtaga atttaagagt ttaatggatt atgcaatgaa gtttaaattt gatgattggt
     3601 tcccgctttt atgtgataac tcggcgtatg ttattcaaga atatataaaa tcaaatcggt
     3661 ataaatctga ccgatagatt ttgaatttaa gagtgtcaca agacactctt ttttcgcacc
     3721 aacgaaaact ggtttaagcc gactgcgcaa aagacataat cgataagctt taatgcggta
     3781 gtttatcaca gttaaattgc taacgcagtc aggcaccgtg tatgaaatct aacaatgcgc
     3841 tcatcgtcat cctcggcacc gtcaccctgg atgctgtagg cataggcttg gttatgccgg
     3901 tactgccggg cctcttgcgg gatcgaggcc tttagcatat tatgttgcca actgtcggaa
     3961 cgagacttct ctaaaccttc ccggcttcat catgctctct taacactgct acatcaaccc
     4021 cttgaatcct caattcacct cctcgaaaac atttcccatc aatcatattc tggtatatct
     4081 tatcttctgg cagtgacagc gtcacttcgt aatcattatg attgatgata atcaaatact
     4141 tccattcatc agtctctctt tgttgcacct ctacattttc agctacttca agaatgggat
     4201 taatatgatg tttagcgaaa acctgttcta aaagcctgcc taaataatta ctatctggat
     4261 aagtaccgac gtaaatcccc tctcctttac cgtagcagtt acgtgtaacc gccggaagtc
     4321 ctgcatacca atccccctta aatgtcgcta gaggttctgc cccttctaat cggattatgt
     4381 ccgcccacgt cgtacaatca tattccccat cgttactata tattttgttt acctttgttt
     4441 ctgggtatgg tacaaattcc tcaacaaaaa tccccaaaat atctcgcaga ggaccaggat
     4501 atccgcctag atgtacacgg tcattttcat ctacaatgcc actgaagaaa ctgacaatca
     4561 aagtgccacc gttagcaaca aattgccgta agttttcatc ttctccctct ttaaccatat
     4621 ataacattgg agcaataact actttgtatt ttgttagatc atcagatggc cttacaaaat
     4681 cgacagcaat attacgttta tataattccc tataataagc ttcaactata ggaatatatc
     4741 ttagtttatt atgtggtttg gaacttagtt cgacagccca ccagttttcc caatcaaaaa
     4801 tgatcgcgac ctctgccttg attctagatc cgaccaaaca atctaacttt ttcagctctt
     4861 gccctaactg tgtaacttcc ctataaattc tattattctc gttcaaaaag tggggcacca
     4921 ttgcaccgtg gaatttttca gctcctgctc tactttgacg ccactggaaa aacataatac
     4981 catctgcacc acgggcaata gttgcataac tccatagacg cattacacct ggcggttttg
     5041 gaacattaat atcgcgccag ttaacatgtg aggttacctg ctccatcaaa ataaacggtt
     5101 gaccttttct taaactacgc ataaggtcat tcatcatggc gtgctgaatt ggcaagccct
     5161 ctctgggatc aggatatgag tcccatgtca caatatctac atgctgagcc cattgaaaat
     5221 agtttaacgg tttgaatgaa cccatgaaat tagttgatac tggaatatct ggtgttacct
     5281 cacgtaaaat ttccttttct gttaaaaaca acttgagaat tgagtcattc ataaaacggt
     5341 agtaatcaag ttcttgggat ggattaataa aagttggtgc ctttctaggg ggattaattt
     5401 catcccaatg attgtatcgc tgtccccaaa agtttgtacc ccaacgttca tttaattcat
     5461 cgattgtttt atatctttcc tttagccact ttctaaacgc gacagcacaa ttctcacaaa
     5521 aacacttgga aacgtgacat gcatactcat tattaacatg ccacattttg agtgccggat
     5581 gatttttata ccgttctgct atagccctca caagtctctt tatgtgcgta attaattgag
     5641 gatgattagg acaataatgt tgtctactgc caaacgagag aatgacaccg ctttcatcga
     5701 tcggcaaaga atctggatac ttttttacaa accaagctgg agtagttgca gtcgccgtcc
     5761 ccaagttaat ataaacaccg tggtcatata gtatatctat aaccttgtct agccattcaa
     5821 agtcgaacac tccatcagac ggttcgatct tgctccaact gaaaatccct aaagatacta
     5881 aattcacccc cgctttttgc atcaacttag catcttcata ccaaatttcc tctggccatt
     5941 gctctgggtt ataatctcct ccgtaacaaa ttgaggataa cacattcata attattcccc
     6001 ctagctaatt ttcgtttaat tataaattaa gttaaaattt aggtacctac gtaggatcga
     6061 tccgacctta attatattaa ggtcccaata taatcattta tcaactcttt tacacttaaa
     6121 tttcctaata tttgtaaata tttttttgaa taaaaaaatc tagctaatgt tacgttacac
     6181 attaactaga cagatctatc gatgcatgcc atggtacccg ggagctcgaa ttctagaagc
     6241 ttctgcagac gcgtcgacgt catatggatc cgatatcgcc cgacgcgagg ctggatggcc
     6301 ttccccatta tgattcttct cgcttccggc ggcatcggga tgcccgcgtt gcaggccatg
     6361 ctgtccaggc aggtagatga cgaccatcag ggacagcttc aaggatcgct cgcggctctt
     6421 accagcctaa cttcgatcat tggaccgctg atcgtcacgg cgatttatgc cgcctcggcg
     6481 agcacatgga acgggttggc atggattgta ggcgccgccc tataccttgt ctgcctcccc
     6541 gcgttgcgtc gcggtgcatg gagccgggcc acctcgacct gaatggaagc cggcggcacc
     6601 tcgctaacgg attcaccact ccaagaattg gagccaatca attcttgcgg agaactgtga
     6661 atgcgcaaac caacccttgg cagaacatat ccatcgcgtc cgccatctcc agcagccgca
     6721 cgcggcgcat ctcgggcagc gttgggtcct ggccacgggt gcgcatgatc gtgctcctgt
     6781 cgttgaggac ccggctaggc tggcggggtt gccttactgg ttagcagaat gaatcaccga
     6841 tacgcgagcg aacgtgaagc gactgctgct gcaaaacgtc tgcgacctga gcaacaacat
     6901 gaatggtctt cggtttccgt gtttcgtaaa gtctggaaac gcggaagtca gcgccctgca
     6961 ccattatgtt ccggatctgc atcgcaggat gctgctggct accctgtgga acacctacat
     7021 ctgtattaac gaagcgctgg cattgaccct gagtgatttt tctctggtcc cgccgcatcc
     7081 ataccgccag ttgtttaccc tcacaacgtt ccagtaaccg ggcatgttca tcatcagtaa
     7141 cccgtatcgt gagcatcctc tctcgtttca tcggtatcat tacccccatg aacagaaatc
     7201 ccccttacac ggaggcatca gtgaccaaac aggaaaaaac cgcccttaac atggcccgct
     7261 ttatcagaag ccagacatta acgcttctgg agaaactcaa cgagctggac gcggatgaac
     7321 aggcagacat ctgtgaatcg cttcacgacc acgctgatga gctttaccgc agctgcctcg
     7381 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
     7441 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
     7501 gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg tatactggct
     7561 taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc
     7621 gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct cgctcactga
     7681 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     7741 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     7801 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     7861 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     7921 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     7981 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     8041 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     8101 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     8161 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     8221 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     8281 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     8341 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     8401 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     8461 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     8521 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     8581 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     8641 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     8701 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     8761 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     8821 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     8881 agttaatagt ttgcgcaacg ttgttgccat tgctgcaggc atcgtggtgt cacgctcgtc
     8941 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     9001 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     9061 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     9121 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     9181 tatgcggcga ccgagttgct cttgcccggc gtcaacacgg gataataccg cgccacatag
     9241 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     9301 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     9361 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     9421 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     9481 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     9541 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
     9601 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct
     9661 tcaa
//
