pELS200 vector (V007577)

LOCUS       V007577                 7828 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V007577
VERSION     V007577
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7828)
  AUTHORS   Diep DB, Johnsborg O, Risoen PA, Nes IF.
  TITLE     Evidence for dual functionality of the operon plnABCD in the
            regulation of the operon plnABCD in the regulation of bacteriocin
            production in Lactobacillus plantarum
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7828)
  AUTHORS   Skaugen M, Axelsson L.
  TITLE     pELS200 E.coli/LActibacillus shuttle vector
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 7828)
  AUTHORS   Skaugen M, Axelsson L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-FEB-2004) Dept. of Chemistry, Biotechnology, and Food
            Science, Agricultural University of Norway, Chr. Magnus Falsens vei
            1, Aas N-1432, Norway
REFERENCE   4  (bases 1 to 7828)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 7828)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
            (25-FEB-2004) Dept. of Chemistry, Biotechnology, and Food Science,
            Agricultural University of Norway, Chr. Magnus Falsens vei 1, Aas
            N-1432, Norway"
            SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7828
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             190..438
                     /gene="parD"
                     /label="Antitoxin ParD"
                     /note="Antitoxin ParD from Escherichia coli. Accession#:
                     P22995"
     CDS             438..746
                     /gene="parE"
                     /label="Toxin ParE"
                     /note="Toxin ParE from Escherichia coli. Accession#:
                     Q79EC5"
     primer_bind     883..899
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             complement(1066..1800)
                     /gene="ermBP"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from Enterococcus
                     faecalis. Accession#: P0A4D5"
     CDS             complement(1897..1980)
                     /codon_start=1
                     /gene="ermBL"
                     /product="ErmBL"
                     /label="ermBL"
                     /note="regulatory protein"
                     /protein_id="AAS77684.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            complement(1897..1980)
                     /gene="ermBL"
                     /label="ermBL"
     oriT            complement(3275..3383)
                     /direction=LEFT
                     /label="oriT"
                     /note="incP origin of transfer"
     promoter        3560..3664
                     /label="AmpR promoter"
     CDS             3665..4522
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      4772..5302
                     /label="oriV"
                     /note="incP origin of replication"
     regulatory      5524..5642
                     /note="Pneo; neomycin phosphotransferase promoter"
                     /regulatory_class="promoter"
     CDS             5694..6839
                     /label="trfA"
                     /note="trans-acting replication protein that binds to and
                     activates oriV"
     primer_bind     7271..7287
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        7297..7315
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    complement(7324..7431)
                     /label="MCS"
                     /note="pBluescript multiple cloning site"
     promoter        complement(7444..7462)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7483..7499)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(7507..7523)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7531..7561)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7576..7597)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."