LOCUS       V007577                 7828 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V007577
VERSION     V007577
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7828)
  AUTHORS   Diep DB, Johnsborg O, Risoen PA, Nes IF.
  TITLE     Evidence for dual functionality of the operon plnABCD in the
            regulation of the operon plnABCD in the regulation of bacteriocin
            production in Lactobacillus plantarum
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7828)
  AUTHORS   Skaugen M, Axelsson L.
  TITLE     pELS200 E.coli/LActibacillus shuttle vector
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 7828)
  AUTHORS   Skaugen M, Axelsson L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-FEB-2004) Dept. of Chemistry, Biotechnology, and Food
            Science, Agricultural University of Norway, Chr. Magnus Falsens vei
            1, Aas N-1432, Norway
REFERENCE   4  (bases 1 to 7828)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 7828)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
            (25-FEB-2004) Dept. of Chemistry, Biotechnology, and Food Science,
            Agricultural University of Norway, Chr. Magnus Falsens vei 1, Aas
            N-1432, Norway"
            SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7828
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             190..438
                     /gene="parD"
                     /label="Antitoxin ParD"
                     /note="Antitoxin ParD from Escherichia coli. Accession#:
                     P22995"
     CDS             438..746
                     /gene="parE"
                     /label="Toxin ParE"
                     /note="Toxin ParE from Escherichia coli. Accession#:
                     Q79EC5"
     primer_bind     883..899
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             complement(1066..1800)
                     /gene="ermBP"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from Enterococcus
                     faecalis. Accession#: P0A4D5"
     CDS             complement(1897..1980)
                     /codon_start=1
                     /gene="ermBL"
                     /product="ErmBL"
                     /label="ermBL"
                     /note="regulatory protein"
                     /protein_id="AAS77684.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            complement(1897..1980)
                     /gene="ermBL"
                     /label="ermBL"
     oriT            complement(3275..3383)
                     /direction=LEFT
                     /label="oriT"
                     /note="incP origin of transfer"
     promoter        3560..3664
                     /label="AmpR promoter"
     CDS             3665..4522
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      4772..5302
                     /label="oriV"
                     /note="incP origin of replication"
     regulatory      5524..5642
                     /note="Pneo; neomycin phosphotransferase promoter"
                     /regulatory_class="promoter"
     CDS             5694..6839
                     /label="trfA"
                     /note="trans-acting replication protein that binds to and
                     activates oriV"
     primer_bind     7271..7287
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        7297..7315
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    complement(7324..7431)
                     /label="MCS"
                     /note="pBluescript multiple cloning site"
     promoter        complement(7444..7462)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7483..7499)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(7507..7523)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7531..7561)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7576..7597)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
ORIGIN
        1 gatctgcgcg tcccccttgg tcaaattggg tatacccatt tgggcctagt ctagccggca
       61 tggcgcatta cagcaatacg caatttaaat gcgcctagcg cattttcccg accttaatgc
      121 gcctcgcgct gtagcctcac gcccacatat gtgctaatgt ggttacgtgt attttatgga
      181 ggttatccaa tgagccgcct gacaatcgac atgacggacc agcagcacca gagcctgaaa
      241 gccctggccg ccttgcaggg caagaccatt aagcaatacg ccctcgaacg tctgttcccc
      301 ggtgacgctg atgccgatca ggcatggcag gaactgaaaa ccatgctggg gaaccgcatc
      361 aacgatgggc ttgccggcaa ggtgtccacc aagagcgtcg gcgaaattct tgatgaagaa
      421 ctcagcgggg atcgcgcttg acggcctaca tcctcacggc tgaggccgaa gccgatctac
      481 gcggcatcat ccgctacacg cgccgggagt ggggcgcggc gcaggtgcgc cgctatatcg
      541 ctaagctgga acagggcata gccaggcttg ccgccggcga aggcccgttt aaggacatga
      601 gcgaactctt tcccgcgctg cggatggccc gctgcgaaca ccactacgtt ttttgcctgc
      661 cgcgtgcggg cgaacccgcg ttggtcgtgg cgatcctgca tgagcgcatg gacctcatga
      721 cgcgacttgc cgacaggctc aagggctgat ttcagccgct aaaaatcgcg ccactcacaa
      781 cgtcctgatg gcgtactaag ctgggggatc tgctggcgaa agggggatgt gctgcaaggc
      841 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg
      901 aattaattct tgaagacgaa aggcgaaccc cgatacaaat tccccgtagg cgctagggac
      961 ctctttagct tcttggaagc tgtcagtagt atatctaata atttatctcc attcccttta
     1021 gtaacgtgta actttccaaa tttaaaaaag cgactcatag aattatttcc tcccgttaaa
     1081 taatagataa ctattaaaaa tagacaatac ttgctcataa gtaatggtac ttaaattgtt
     1141 tactttggcg tgtttcattg cttgacgaaa ctgattttta gtaaacagtt gacgatattc
     1201 tcgattgacc cattttgaaa caaagtactt atatagcttc caatatttat ctggaacatc
     1261 tgtggtatgg cgggtaagtt ttattaagac actgtttact tttggtttag gatgaaagca
     1321 ttccgctggc agcttaagca attgctgaat cgagacttga gtgtgcaaga gcaaccctag
     1381 tgttcggtga atatccaagg tacgcttgta gaatccttct tcaacagtca gatagatgtc
     1441 agacgcacgg ctttcaaaaa ccactttttt aataatttgt gtgcttaaat tgtaaggaat
     1501 attcccaaca attttatacc tctgtttgtt agggaattga aactgtagaa tatcttggtg
     1561 aattaaagtg acacgagtat tcagttttaa tttttctgac gataagttga atagatgact
     1621 gtctaattca atagacgtta cctgtttact tattttagcc agtttcgtcg ttaaatgccc
     1681 tttacctgtt ccaatttcgt aaacggtatc ggtttctttt aaattcaatt gttttattat
     1741 ttggttgagt actttttcac tcgttaaaaa gttttgagaa tattttatat ttttgttcat
     1801 gtaatctctc ctgaagttaa acgatttgtt tgtaatttta gttatctgtt taaaaagtca
     1861 taagattagt cactggtagg aattaatcta acgtatttat ttatctgcgt aatcactgtt
     1921 tttagtctgt ttcaaaacag tagatgtttt atctacatta cgcatttgga ataccaacat
     1981 gacgaatccc tccttcttaa ttacaaattt ttagcatcta atttaacttc aattcctatt
     2041 tttagcatct aatttaactt caattcctat tatacaaaat tttaagataa tgcactatca
     2101 acacactctt aagtttgctt ctaaggggta ccgaattcct agtatattaa ataacacctt
     2161 aagtctagca ccaacccgca cgcatagcgg tgcttaaacc atcaagggtc aagcccttag
     2221 gctctctcaa acagttatcc taatcgtgaa taactgcgct tcttttgcag tataaagaga
     2281 gaactcttta tcagacaatt taagctcaac cagcccttgc actaactatt attagagttg
     2341 gttttagcag caacccgaat aatctgcgtt aatagttagc ctgtccgtat catttcctag
     2401 tcttccagcc acgtctttag tcgcgttgat ctcgacaagg tttagcatac ctatgttgtt
     2461 aactgcaagc ggggtcacga acgacactca cgggaggttt tactagctaa gaacaggttt
     2521 ccagccttta gttgctttga tggttgctaa ccattgaata acaaaaaaac ggttgctatc
     2581 aggtttctgt taagattccc gataacaacc gtttacttta agtatcaatg gttgaaaaac
     2641 ttagcctaca tgttataata gtaccaagtt agatagcttg tattggtagt acttgctatc
     2701 gaaaatctta tcaggttgtg ctgataagtc gtgaatccta actctgctaa gttgagggtt
     2761 cttttttttg cgttcattta ttaagttgag tacattataa ccgtaatata agattaatac
     2821 aacctttatc attttaacgt ctcaaccagc cgaataatcc ttttttaagc gttttagttt
     2881 cttccccctt gtaatgatcc ccagtgggtt ctaaaacgtc tgtatcgtcc tttgaggcgt
     2941 cctcgctggt cttttgtggg gcatcgagta gtttttcatt ttgacggtta gtggataagt
     3001 ttaattgctg ttgctgatcc tctaatttgt taaatcagct cattttttaa ccaataggcc
     3061 gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt
     3121 ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa
     3181 accgtctatc agggcgatgg cccactacaa gggcctcgtg atacgcctat ttttataggt
     3241 taatgtcatg ataataatgg tttcttagag cttacggcca gcctcgcaga gcaggattcc
     3301 cgttgagcac cgccaggtgc gaataaggga cagtgaagaa ggaacacccg ctcgcgggtg
     3361 ggcctacttc acctatcctg cccggctgac gccgttggat acaccaagga aagtctacac
     3421 gaaccctttg gcaaaatcct gtatatcgtg cgaaaaagga tggatatacc gaaaaaatcg
     3481 ctataatgac cccgaagcag ggttatgcag cggaaaagat ccgtcgatcg acccaggtgg
     3541 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     3601 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     3661 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     3721 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     3781 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     3841 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     3901 atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     3961 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     4021 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     4081 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     4141 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     4201 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     4261 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     4321 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     4381 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     4441 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     4501 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     4561 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     4621 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     4681 gatcaaagga tcttcttgag atcctttttt tctgcggggg atcaggaccg ctgccggagc
     4741 gcaacccact cactacagca gagccatgta gggccgccgg cgttgtggat accacgcgga
     4801 aaacttggcc ctcactgaca gatgaggggc ggacgttgac acttgagggg ccgactcacc
     4861 cggcgcggcg ttgacagatg aggggcaggc tcgatttcgg ccggcgacgt ggagctggcc
     4921 agcctcgcaa atcggcgaaa acgcctgatt ttacgcgagt ttcccacaga tgatgtggac
     4981 aagcctgggg ataagtgccc tgcggtattg acacttgagg ggcgcgacta ctgacagatg
     5041 aggggcgcga tccttgacac ttgaggggca gagtgatgac agatgagggg cgcacctatt
     5101 gacatttgag gggctgtcca caggcagaaa atccagcatt tgcaagggtt tccgcccgtt
     5161 tttcggccac cgctaacctg tcttttaacc tgcttttaaa ccaatattta taaaccttgt
     5221 ttttaaccag ggctgcgccc tggcgcgtga ccgcgcacgc cgaagggggg tgccccccct
     5281 tctcgaaccc tcccggcccg ctaacgcggc accccatccc cccaggggct gcgcccctcg
     5341 gccgcgaacg acctcacccc aaaaatggca gccacgtaga aagccagtcc gcagaaacgg
     5401 tgctgacccc ggatgaatgt cagctactgg gctatctgga caagggaaaa cgcaagcgca
     5461 aagagaaagc aggtagcttg cagtgggctt acatggcgat agctagactg ggcggtttta
     5521 tggacagcaa gcgaaccgga attgccagct ggggcgccct ctggtaaggt tgggaagccc
     5581 tgcaaagtaa actggatggc tttcttgccg ccaaggatct gatggcgcag gggatcaaga
     5641 tcgacggatc gatccgggga attaattccg gggcaatccc gcaaggaggg tgaatgaatc
     5701 ggacgtttga ccggaaggca tacaggcaag aactgatcga cgcggggttt tccgccgagg
     5761 atgccgaaac catcgcaagc cgcaccgtca tgcgtgcgcc ccgcgaaacc ttccagtccg
     5821 tcggctcgat ggtccagcaa gctacggcca agatcgagcg cgacagcgtg caactggctc
     5881 cccctgccct gcccgcgcca tcggccgccg tggagcgttc gcgtcgtctc gaacaggagg
     5941 cggcaggttt ggcgaagtcg atgaccatcg acacgcgagg aactatgacg accaagaagc
     6001 gaaaaaccgc cggcgaggac ctggcaaaac aggtcagcga ggccaagcag gccgcgttgc
     6061 tgaaacacac gaagcagcag atcaaggaaa tgcagctttc cttgttcgat attgcgccgt
     6121 ggccggacac gatgcgagcg atgccaaacg acacggcccg ctctgccctg ttcaccacgc
     6181 gcaacaagaa aatcccgcgc gaggcgctgc aaaacaaggt cattttccac gtcaacaagg
     6241 acgtgaagat cacctacacc ggcgtcgagc tgcgggccga cgatgacgaa ctggtgtggc
     6301 agcaggtgtt ggagtacgcg aagcgcaccc ctatcggcga gccgatcacc ttcacgttct
     6361 acgagctttg ccaggacctg ggctggtcga tcaatggccg gtattacacg aaggccgagg
     6421 aatgcctgtc gcgcctacag gcgacggcga tgggcttcac gtccgaccgc gttgggcacc
     6481 tggaatcggt gtcgctgctg caccgcttcc gcgtcctgga ccgtggcaag aaaacgtccc
     6541 gttgccaggt cctgatcgac gaggaaatcg tcgtgctgtt tgctggcgac cactacacga
     6601 aattcatatg ggagaagtac cgcaagctgt cgccgacggc ccgacggatg ttcgactatt
     6661 tcagctcgca ccgggagccg tacccgctca agctggaaac cttccgcctc atgtgcggat
     6721 cggattccac ccgcgtgaag aagtggcgcg agcaggtcgg cgaagcctgc gaagagttgc
     6781 gaggcagcgg cctggtggaa cacgcctggg tcaatgatga cctggtgcat tgcaaacgct
     6841 agggccttgt ggggtcagtt ccggctgggg gttcagcacc tgcatgatta ttgaagcatt
     6901 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     6961 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg ccggcgaacg tggcgagaaa
     7021 ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct
     7081 gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt ccattcgcca
     7141 ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag
     7201 ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag
     7261 tcacgacgtt gtaaaacgac ggccagtgag cgcgcgtaat acgactcact atagggcgaa
     7321 ttgggtaccg ggccccccct cgaggtcgac ggtatcgata agcttgatat cgaattcctg
     7381 cagcccgggg gatccactag ttctagagcg gccgccaccg cggtggagct ccagcttttg
     7441 ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt
     7501 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     7561 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     7621 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     7681 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     7741 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     7801 atcaggggat aacgcaggaa agaacatg
//
