pELR-1 vector (V007579)

LOCUS       V007579                 9936 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V007579
VERSION     V007579
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9936)
  AUTHORS   Weber E, Engler C, Gruetzner R, Werner S, Marillonnet S.
  TITLE     A modular cloning system for standardized assembly of multigene
            constructs
  JOURNAL   PLoS ONE 6 (2), E16765 (2011)
   PUBMED   21364738
REFERENCE   2  (bases 1 to 9936)
  AUTHORS   Weber E, Engler C, Gruetzner R, Werner S, Marillonnet S.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-DEC-2010) Icon Genetics GmbH, Weinbergweg 22,
            Halle/Saale 06120, Germany
REFERENCE   3  (bases 1 to 9936)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9936)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE";
            date: "2011"; volume: "6"; issue: "2"; pages: "E16765"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (20-DEC-2010) Icon Genetics GmbH, Weinbergweg 22, Halle/Saale 06120,
            Germany"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9936
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             228..1133
                     /gene="crtE"
                     /label="Geranylgeranyl diphosphate synthase"
                     /note="Geranylgeranyl diphosphate synthase from Pantoea
                     ananas. Accession#: P21684"
     CDS             1146..1874
                     /codon_start=1
                     /gene="crtW"
                     /product="beta-carotene ketolase"
                     /label="crtW"
                     /protein_id="ADZ30896.1"
                     /translation="MSAHALPKADLTATSLIVSGGIIAAWLALHVHALWFLDAAAHPIL
                     AIANFLGLTWLSVGLFIIAHDAMHGSVVPGRPRANAAMGQLVLWLYAGFSWRKMIVKHM
                     AHHRHAGTDDDPDFDHGGPVRWYARFIGTYFGWREGLLLPVIVTVYALILGDRWMYVVF
                     WPLPSILASIQLFVFGTWLPHRPGHDAFPDRHNARSSRISDPVSLLTCFHFGGYHHEHH
                     LHPTVPWWRLPSTRTKGDTA"
     gene            1146..1874
                     /gene="crtW"
                     /label="crtW"
     regulatory      1502..1511
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1896..3041
                     /gene="crtY"
                     /label="Lycopene beta-cyclase"
                     /note="Lycopene beta-cyclase from Pantoea ananas.
                     Accession#: P21687"
     CDS             3056..4531
                     /gene="crtI"
                     /label="Phytoene desaturase (lycopene-forming)"
                     /note="Phytoene desaturase (lycopene-forming) from Pantoea
                     ananas. Accession#: P21685"
     CDS             4531..5457
                     /gene="crtB"
                     /label="15-cis-phytoene synthase"
                     /note="15-cis-phytoene synthase from Pantoea ananas.
                     Accession#: P21683"
     misc_feature    5577..5580
                     /note="fusion site; other site"
     misc_feature    5615..5639
                     /label="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             complement(5798..6943)
                     /label="trfA"
                     /note="trans-acting replication protein that binds to and
                     activates oriV"
     rep_origin      complement(7279..7867)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(8041..8898)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(8899..9003)
                     /label="AmpR promoter"
     rep_origin      complement(9020..9729)
                     /direction=LEFT
                     /label="oriV"
                     /note="incP origin of replication"
     misc_feature    9813..9837
                     /label="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
     misc_feature    9926..9929
                     /note="fusion site; other site"