LOCUS       V007579                 9936 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V007579
VERSION     V007579
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9936)
  AUTHORS   Weber E, Engler C, Gruetzner R, Werner S, Marillonnet S.
  TITLE     A modular cloning system for standardized assembly of multigene
            constructs
  JOURNAL   PLoS ONE 6 (2), E16765 (2011)
   PUBMED   21364738
REFERENCE   2  (bases 1 to 9936)
  AUTHORS   Weber E, Engler C, Gruetzner R, Werner S, Marillonnet S.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-DEC-2010) Icon Genetics GmbH, Weinbergweg 22,
            Halle/Saale 06120, Germany
REFERENCE   3  (bases 1 to 9936)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9936)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE";
            date: "2011"; volume: "6"; issue: "2"; pages: "E16765"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (20-DEC-2010) Icon Genetics GmbH, Weinbergweg 22, Halle/Saale 06120,
            Germany"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9936
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             228..1133
                     /gene="crtE"
                     /label="Geranylgeranyl diphosphate synthase"
                     /note="Geranylgeranyl diphosphate synthase from Pantoea
                     ananas. Accession#: P21684"
     CDS             1146..1874
                     /codon_start=1
                     /gene="crtW"
                     /product="beta-carotene ketolase"
                     /label="crtW"
                     /protein_id="ADZ30896.1"
                     /translation="MSAHALPKADLTATSLIVSGGIIAAWLALHVHALWFLDAAAHPIL
                     AIANFLGLTWLSVGLFIIAHDAMHGSVVPGRPRANAAMGQLVLWLYAGFSWRKMIVKHM
                     AHHRHAGTDDDPDFDHGGPVRWYARFIGTYFGWREGLLLPVIVTVYALILGDRWMYVVF
                     WPLPSILASIQLFVFGTWLPHRPGHDAFPDRHNARSSRISDPVSLLTCFHFGGYHHEHH
                     LHPTVPWWRLPSTRTKGDTA"
     gene            1146..1874
                     /gene="crtW"
                     /label="crtW"
     regulatory      1502..1511
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1896..3041
                     /gene="crtY"
                     /label="Lycopene beta-cyclase"
                     /note="Lycopene beta-cyclase from Pantoea ananas.
                     Accession#: P21687"
     CDS             3056..4531
                     /gene="crtI"
                     /label="Phytoene desaturase (lycopene-forming)"
                     /note="Phytoene desaturase (lycopene-forming) from Pantoea
                     ananas. Accession#: P21685"
     CDS             4531..5457
                     /gene="crtB"
                     /label="15-cis-phytoene synthase"
                     /note="15-cis-phytoene synthase from Pantoea ananas.
                     Accession#: P21683"
     misc_feature    5577..5580
                     /note="fusion site; other site"
     misc_feature    5615..5639
                     /label="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             complement(5798..6943)
                     /label="trfA"
                     /note="trans-acting replication protein that binds to and
                     activates oriV"
     rep_origin      complement(7279..7867)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(8041..8898)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(8899..9003)
                     /label="AmpR promoter"
     rep_origin      complement(9020..9729)
                     /direction=LEFT
                     /label="oriV"
                     /note="incP origin of replication"
     misc_feature    9813..9837
                     /label="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
     misc_feature    9926..9929
                     /note="fusion site; other site"
ORIGIN
        1 gagggtaccg cacggtctgc caatccgacg gaggtttatg aattttccac cttttccaca
       61 agctcaacta gtattaacga tgtggattta gcaaaaaaaa cctgtaaccc taaatgtaaa
      121 ataacgggta agcctgccaa ccatgttatg gcagattaag cgtctttttg aagggcaccg
      181 catctttcgc gttgccgtaa atgtatccgt ttataaggac agcccgaatg acggtctgcg
      241 caaaaaaaca cgttcatctc actcgcgatg ctgcggagca gttactggct gatattgatc
      301 gacgccttga tcagttattg cccgtggagg gagaacggga tgttgtgggt gccgcgatgc
      361 gtgaaggtgc gctggcaccg ggaaaacgta ttcgccccat gttgctgttg ctgaccgccc
      421 gcgatctggg ttgcgctgtc agccatgacg gattactgga tttggcctgt gcggtggaaa
      481 tggtccacgc ggcttcgctg atccttgacg atatgccctg catggacgat gcgaagctgc
      541 ggcgcggacg ccctaccatt cattctcatt acggagagca tgtggcaata ctggcggcgg
      601 ttgccttgct gagtaaagcc tttggcgtaa ttgccgatgc agatggcctc acgccgctgg
      661 caaaaaatcg ggcggtttct gaactgtcaa acgccatcgg catgcaagga ttggttcagg
      721 gtcagttcaa ggatctgtct gaaggggata agccgcgcag cgctgaagct attttgatga
      781 cgaatcactt taaaaccagc acgctgtttt gtgcctccat gcagatggcc tcgattgttg
      841 cgaatgcctc cagcgaagcg cgtgattgcc tgcatcgttt ttcacttgat cttggtcagg
      901 catttcaact gctggacgat ttgaccgatg gcatgaccga caccggtaag gatagcaatc
      961 aggacgccgg taaatcgacg ctggtcaatc tgttaggccc gagggcggtt gaagaacgtc
     1021 tgagacaaca tcttcagctt gccagtgagc atctctctgc ggcctgccaa cacgggcacg
     1081 ccactcaaca ttttattcag gcctggtttg acaaaaaact cgctgccgtc agttaaggat
     1141 gctgcatgag cgcacatgcc ctgcccaagg cagatctgac cgccaccagc ctgatcgtat
     1201 cgggcggcat catcgccgct tggctggccc tgcatgtgca tgcgctgtgg tttctggacg
     1261 cagcggcgca tcccatcctg gcgatcgcaa atttcctggg gctgacctgg ctgtcggtcg
     1321 gattgttcat catcgcgcat gacgcgatgc acgggtcggt ggtgccgggg cgtccgcgcg
     1381 ccaatgcggc gatgggccag cttgtcctgt ggctgtatgc cggattttcg tggcgcaaga
     1441 tgatcgtcaa gcacatggcc catcaccgcc atgccggaac cgacgacgac cccgatttcg
     1501 accatggcgg cccggtccgc tggtacgccc gcttcatcgg cacctatttc ggctggcgcg
     1561 aggggctgct tctgcccgtc atcgtgacgg tctatgcgct gatccttggg gatcgctgga
     1621 tgtacgtggt attctggccg ctgccgtcga tcctggcgtc gatccagctg ttcgtgttcg
     1681 gcacctggct gccgcaccgc cccggccacg acgcgttccc ggaccgccac aatgcgcggt
     1741 cgtcgcggat cagcgacccc gtgtcgctgc tgacctgttt tcactttggc ggttatcatc
     1801 acgaacacca cctgcacccg acggtgccgt ggtggcgcct gcccagcacc cgcaccaagg
     1861 gggacaccgc atgagtgact taagtgggag cggctatgca accgcattat gatctgattc
     1921 tcgtgggggc tggactcgcg aatggcctta tcgccctgcg acttcagcag cagcaacctg
     1981 atatgcgtat tttgcttatc gacgccgcac cccaggcggg cgggaatcat acgtggtcat
     2041 ttcaccacga tgatttgact gagagccaac atcgttggat agctccgctg gtggttcatc
     2101 actggcccga ctatcaggta cgctttccca cacgccgtcg taagctgaac agcggctact
     2161 tttgtattac ttctcagcgt ttcgctgagg ttttacagcg acagtttggc ccgcacttgt
     2221 ggatggatac cgcggtcgca gaggttaatg cggaatctgt tcggttgaaa aagggtcagg
     2281 ttatcggtgc ccgcgcggtg attgacgggc ggggttatgc ggcaaattca gcactgagcg
     2341 tgggcttcca ggcgtttatt ggccaggaat ggcgattgag ccacccgcat ggtttatcat
     2401 ctcccattat catggatgcc acggtcgatc agcaaaatgg ttatcgcttc gtgtacagcc
     2461 tgccgctctc gccgaccaga ttgttaattg aagatacgca ctatattgat aatgcgacat
     2521 tagatcctga atgcgcgcgg caaaatattt gcgactatgc cgcgcaacag ggttggcagc
     2581 ttcagacact gctgcgagaa gaacagggcg ccttacccat tactctgtcg ggcaatgccg
     2641 acgcattctg gcagcagcgc cccctggcct gtagtggatt acgtgccggt ctgttccatc
     2701 ctaccaccgg ctattcactg ccgctggcgg ttgccgtggc cgaccgcctg agtgcacttg
     2761 atgtctttac gtcggcctca attcaccatg ccattacgca ttttgcccgc gagcgctggc
     2821 agcagcaggg ctttttccgc atgctgaatc gcatgctgtt tttagccgga cccgccgatt
     2881 cacgctggcg ggttatgcag cgtttttatg gtttacctga agatttaatt gcccgttttt
     2941 atgcgggaaa actcacgctg accgatcggc tacgtattct gagcggcaag ccgcctgttc
     3001 cggtattagc agcattgcaa gccattatga cgactcatcg ttaaagagcg actacatgaa
     3061 accaactacg gtaattggtg caggcttcgg tggcctggca ctggcaattc gtctacaagc
     3121 tgcggggatc cccgtcttac tgcttgaaca acgtgataaa cccggcggtc gggcttatgt
     3181 ctacgaggat caggggttta cctttgatgc aggcccgacg gttatcaccg atcccagtgc
     3241 cattgaagaa ctgtttgcac tggcaggaaa acagttaaaa gagtatgtcg aactgctgcc
     3301 ggttacgccg ttttaccgcc tgtgttggga gtcagggaag gtctttaatt acgataacga
     3361 tcaaacccgg ctcgaagcgc agattcagca gtttaatccc cgcgatgtcg aaggttatcg
     3421 tcagtttctg gactattcac gcgcggtgtt taaagaaggc tatctaaagc tcggtactgt
     3481 ccctttttta tcgttcagag acatgcttcg cgccgcacct caactggcga aactgcaggc
     3541 atggagaagc gtttacagta aggttgccag ttacatcgaa gatgaacatc tgcgccaggc
     3601 gttttctttc cactcgctgt tggtgggcgg caatcccttc gccacctcat ccatttatac
     3661 gttgatacac gcgctggagc gtgagtgggg cgtctggttt ccgcgtggcg gcaccggcgc
     3721 attagttcag gggatgataa agctgtttca ggatctgggt ggcgaagtcg tgttaaacgc
     3781 cagagtcagc catatggaaa cgacaggaaa caagattgaa gccgtgcatt tagaggacgg
     3841 tcgcaggttc ctgacgcaag ccgtcgcgtc aaatgcagat gtggttcata cctatcgcga
     3901 cctgttaagc cagcaccctg ccgcggttaa gcagtccaac aaactgcaga ctaagcgcat
     3961 gagtaactct ctgtttgtgc tctattttgg tttgaatcac catcatgatc agctcgcgca
     4021 tcacacggtt tgtttcggcc cgcgttaccg cgagctgatt gacgaaattt ttaatcatga
     4081 tggcctcgca gaggacttct cactttatct gcacgcgccc tgtgtcacgg attcgtcact
     4141 ggcgcctgaa ggttgcggca gttactatgt gttggcgccg gtgccgcatt taggcaccgc
     4201 gaacctcgac tggacggttg aggggccaaa actacgcgac cgtatttttg cgtaccttga
     4261 gcagcattac atgcctggct tacggagtca gctggtcacg caccggatgt ttacgccgtt
     4321 tgattttcgc gaccagctta atgcctatca tggctcagcc ttttctgtgg agcccgttct
     4381 tacccagagc gcctggtttc ggccgcataa ccgcgataaa accattacta atctctacct
     4441 ggtcggcgca ggcacgcatc ccggcgcagg cattcctggc gtcatcggct cggcaaaagc
     4501 gacagcaggt ttgatgctgg aggatctgat ttgaataatc cgtcgttact caatcatgcg
     4561 gtcgaaacga tggcagttgg ctcgaaaagt tttgcgacag cctcaaagtt atttgatgca
     4621 aaaacccggc gcagcgtact gatgctctac gcctggtgcc gccattgtga cgatgttatt
     4681 gacgatcaga cgctgggctt tcaggcccgg cagcctgcct tacaaacgcc cgaacaacgt
     4741 ctgatgcaac ttgagatgaa aacgcgccag gcctatgcag gatcgcagat gcacgaaccg
     4801 gcgtttgcgg cttttcagga agtggctatg gctcatgata tcgccccggc ttacgcgttt
     4861 gatcatctgg aaggcttcgc catggatgta cgcgaagcgc aatacagcca actggatgat
     4921 acgctgcgct attgctatca cgttgcaggc gttgtcggct tgatgatggc gcaaatcatg
     4981 ggcgtgcggg ataacgccac gctggaccgc gcctgtgacc ttgggctggc atttcagttg
     5041 accaatattg ctcgcgatat tgtggacgat gcgcatgcgg gccgctgtta tctgccggca
     5101 agctggctgg agcatgaagg tctgaacaaa gagaattatg cggcacctga aaaccgtcag
     5161 gcgctgagcc gtatcgcccg tcgtttggtg caggaagcag aaccttacta tttgtctgcc
     5221 acagccggcc tggcagggtt gcccctgcgt tccgcctggg caatcgctac ggcgaagcag
     5281 gtttaccgga aaataggtgt caaagttgaa caggccggtc agcaagcctg ggatcagcgg
     5341 cagtcaacga ccacgcccga aaaattaacg ctgctgctgg ccgcctctgg tcaggccctt
     5401 acttcccgga tgcgggctca tcctccccgc cctgcgcatc tctggcagcg cccgctctag
     5461 cgccatgtct ttcccggagc gtcgcctgaa gttttgacag gggcggcgca tagaggaagc
     5521 caaaagaaac acaaccttct ttgcccctga cggcgtgatg catacgctgc gtctcaggga
     5581 ttgtcttcac gaagtggttt aaactatcag tgtttgacag gatatattgg cgggtaaacc
     5641 taagagaaaa gagcgtttat tagaataatc ggatatttaa aagggcgtga aaaggtttat
     5701 ccgttcgtcc atttgtatgt gcatgccaac cacagggttc cccagatcag gcgctggctg
     5761 ctgaaccccc agccggaact gaccccacaa ggccctagcg tttgcaatgc accaggtcat
     5821 cattgaccca ggcgtgttcc accaggccgc tgcctcgcaa ctcttcgcag gcttcgccga
     5881 cctgctcgcg ccacttcttc acgcgggtgg aatccgatcc gcacatgagg cggaaggttt
     5941 ccagcttgag cgggtacggc tcccggtgcg agctgaaata gtcgaacatc cgtcgggccg
     6001 tcggcgacag cttgcggtac ttctcccata tgaatttcgt gtagtggtcg ccagcaaaca
     6061 gcacgacgat ttcctcgtcg atcaggacct ggcaacggga cgttttcttg ccacggtcca
     6121 ggacgcggaa gcggtgcagc agcgacaccg attccaggtg cccaacgcgg tcggacgtga
     6181 agcccatcgc cgtcgcctgt aggcgcgaca ggcattcctc ggccttcgtg taataccggc
     6241 cattgatcga ccagcccagg tcctggcaaa gctcgtagaa cgtgaaggtg atcggctcgc
     6301 cgataggggt gcgcttcgcg tactccaaca cctgctgcca caccagttcg tcatcgtcgg
     6361 cccgcagctc gacgccggtg taggtgatct tcacgtcctt gttgacgtgg aaaatgacct
     6421 tgttttgcag cgcctcgcgc gggattttct tgttgcgcgt ggtgaacagg gcagagcggg
     6481 ccgtgtcgtt tggcatcgct cgcatcgtgt ccggccacgg cgcaatatcg aacaaggaaa
     6541 gctgcatttc cttgatctgc tgcttcgtgt gtttcagcaa cgcggcctgc ttggcctcgc
     6601 tgacctgttt tgccaggtcc tcgccggcgg tttttcgctt cttggtcgtc atagttcctc
     6661 gcgtgtcgat ggtcatcgac ttcgccaaac ctgccgcctc ctgttcaaga cgacgcgaac
     6721 gctccacggc ggccgatggc gcgggcaggg cagggggagc cagttgcacg ctgtcgcgct
     6781 cgatcttggc cgtagcttgc tggaccatcg agccgacgga ctggaaggtt tcgcggggcg
     6841 cacgcatgac ggtgcggctt gcgatggttt cggcatcctc ggcggaaaac cccgcgtcga
     6901 tcagttcttg cctgtatgcc ttccggtcaa acgtccgatt cattcaccct ccttgcggga
     6961 ttgccccgac tcacgccggg gcaatgtgcc cttattcctg atttgacccg cctggtgcct
     7021 tggtgtccag ataatccacc ttatcggcaa tgaagtcggt cccgtagacc gtctggccgt
     7081 ccttctcgta cttggtattc cgaatcttgc cctgcacgaa taccagcgac cccttgccca
     7141 aatacttgcc gtgggcctcg gcctgagagc caaaacactt gatgcggaag aagtcggtgc
     7201 gctcctgctt gtcgccggca tcgttgcgcc acatctagga tctgccagga accgtaaaaa
     7261 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     7321 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     7381 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     7441 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     7501 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     7561 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     7621 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     7681 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     7741 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     7801 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     7861 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     7921 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     7981 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     8041 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     8101 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     8161 tgctgcaatg ataccgcgag aaccacgctc accggctcca gatttatcag caataaacca
     8221 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     8281 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     8341 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     8401 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     8461 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     8521 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     8581 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     8641 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     8701 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     8761 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     8821 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     8881 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     8941 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     9001 gcgcacgaat tggccagcgc tgccattttt ggggtgaggc cgttcgcggc cgaggggcgc
     9061 agcccctggg gggatgggag gcccgcgtta gcgggccggg agggttcgag aagggggggc
     9121 accccccttc ggcgtgcgcg gtcacgcgca cagggcgcag ccctggttaa aaacaaggtt
     9181 tataaatatt ggtttaaaag caggttaaaa gacaggttag cggtggccga aaaacgggcg
     9241 gaaacccttg caaatgctgg attttctgcc tgtggacagc ccctcaaatg tcaataggtg
     9301 cgcccctcat ctgtcagcac tctgcccctc aagtgtcaag gatcgcgccc ctcatctgtc
     9361 agtagtcgcg cccctcaagt gtcaataccg cagggcactt atccccaggc ttgtccacat
     9421 catctgtggg aaactcgcgt aaaatcaggc gttttcgccg atttgcgagg ctggccagct
     9481 ccacgtcgcc ggccgaaatc gagcctgccc ctcatctgtc aacgccgcgc cgggtgagtc
     9541 ggcccctcaa gtgtcaacgt ccgcccctca tctgtcagtg agggccaagt tttccgcgag
     9601 gtatccacaa cgccggcggc cgcggtgtct cgcacacggc ttcgacggcg tttctggcgc
     9661 gtttgcaggg ccatagacgg ccgccagccc agcggcgagg gcaaccagcc cggtgagcgt
     9721 cgcaaaggag atcctgatct gactgatggg ctgcctgtat cgagtggtga ttttgtgccg
     9781 agctgccggt cggggagctg ttggctggct ggtggcagga tatattgtgg tgtaaacaaa
     9841 ttgacgctta gacaacttaa taacacattg cggacgtttt taatgtactg gggtggatgc
     9901 agtgggcccc actctgtgaa gacaagcaat gagacg
//
