pELI3.1 vector (V007580)

LOCUS       40924_17329        3892 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pELI3.1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3892)
  AUTHORS   Yero CD, Pajon FR, Caballero ME, Cobas AK, Lopez HY, Farinas MM, 
            Gonzales BS, Acosta DA.
  TITLE     Immunization of mice with Neisseria meningitidis serogroup B genomic
            expression libraries elicits functional antibodies and reduces the 
            level of bacteremia in an infant rat infection model
  JOURNAL   Vaccine 23 (7), 932-939 (2005)
  PUBMED    15603895
REFERENCE   2  (bases 1 to 3892)
  AUTHORS   Yero D, Pajon R, Caballero E, Cobas K, Lopez Y, Farinas M, Gonzales 
            S, Acosta A.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JUL-2007) Molecular Biology, Finlay Institute, Ave 27,
            Havana City, Havana 11600, Cuba
REFERENCE   3  (bases 1 to 3892)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3892)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Vaccine"; 
            date: "2005"; volume: "23"; issue: "7"; pages: "932-939"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (19-JUL-2007) Molecular Biology, Finlay Institute, Ave 27, Havana 
            City, Havana 11600, Cuba"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3892
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        139..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..729
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          890..1022
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     promoter        1067..1085
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1177..1193
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     regulatory      1207..1216
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    1242..1313
                     /label=multiple cloning site
                     /note="multiple cloning site"
     primer_bind     complement(1314..1330)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(1538..1555)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     polyA_signal    complement(1573..1694)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        2091..2195
                     /label=AmpR promoter
     CDS             2196..3053
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      3227..3815
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"