LOCUS       40924_17329        3892 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pELI3.1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3892)
  AUTHORS   Yero CD, Pajon FR, Caballero ME, Cobas AK, Lopez HY, Farinas MM, 
            Gonzales BS, Acosta DA.
  TITLE     Immunization of mice with Neisseria meningitidis serogroup B genomic
            expression libraries elicits functional antibodies and reduces the 
            level of bacteremia in an infant rat infection model
  JOURNAL   Vaccine 23 (7), 932-939 (2005)
  PUBMED    15603895
REFERENCE   2  (bases 1 to 3892)
  AUTHORS   Yero D, Pajon R, Caballero E, Cobas K, Lopez Y, Farinas M, Gonzales 
            S, Acosta A.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JUL-2007) Molecular Biology, Finlay Institute, Ave 27,
            Havana City, Havana 11600, Cuba
REFERENCE   3  (bases 1 to 3892)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3892)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Vaccine"; 
            date: "2005"; volume: "23"; issue: "7"; pages: "932-939"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (19-JUL-2007) Molecular Biology, Finlay Institute, Ave 27, Havana 
            City, Havana 11600, Cuba"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3892
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        139..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..729
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          890..1022
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     promoter        1067..1085
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1177..1193
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     regulatory      1207..1216
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    1242..1313
                     /label=multiple cloning site
                     /note="multiple cloning site"
     primer_bind     complement(1314..1330)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(1538..1555)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     polyA_signal    complement(1573..1694)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        2091..2195
                     /label=AmpR promoter
     CDS             2196..3053
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      3227..3815
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactg
      661 cgatcgcccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
      721 agcagagctc gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac
      781 agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaactt aagctgcagt
      841 gactctctta aggtagcctt gcagaagttg gtcgtgaggc actgggcagg taagtatcaa
      901 ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac agagaagact
      961 cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac
     1021 aggtgtccac tcccagttca attacagctc ttaaggctag agtacttaat acgactcact
     1081 ataggctagc aagcttcgca attactgtga gttagctcac tcattaggca ccccaggctt
     1141 tacactttat acttccggct cgtatattgt gtggaattgt gagcggataa caatttcaca
     1201 caggaagcca ccatggcctt gattacgccg agcttgcatg cctgcaggtc gactctagac
     1261 gtcagatctg atcaagggat cctgactgac acccgggtac cgagctcgaa ttcactggcc
     1321 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     1381 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     1441 caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc ggtattttct ccttacgcat
     1501 ctgtgcggta tttcacaccg catagggcgg ccgcttccct ttagtgaggg ttaatgcttc
     1561 gagcagacat gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa
     1621 aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct
     1681 gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggaga
     1741 tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtaaaatc cgataaggat
     1801 cgatccgcgt atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc
     1861 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
     1921 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
     1981 caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
     2041 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     2101 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     2161 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     2221 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     2281 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     2341 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     2401 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     2461 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     2521 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     2581 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     2641 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     2701 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     2761 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     2821 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     2881 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     2941 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     3001 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     3061 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     3121 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     3181 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     3241 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     3301 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     3361 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     3421 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     3481 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     3541 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     3601 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     3661 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     3721 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     3781 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     3841 ggttcctggc cttttgctgg ccttttgctc acatggctcg acagatcgat ct
//
