pCotZ-CL-thr vector (V008272)

LOCUS       V008272                 7655 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008272
VERSION     V008272
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7655)
  AUTHORS   Iwanicki A, Hinc K, Stasilojc M, Piatek I, Grela A, Lega T,
            Obuchowski M.
  TITLE     Vector system for spore sufrace display
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7655)
  AUTHORS   Iwanicki A, Hinc K, Stasilojc M, Piatek I, Grela A, Lega T,
            Obuchowski M.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2013) Laboratory of Molecular Bacteriology,
            Medical University of Gdansk, Debinki 1, Gdansk 80-211, Poland
REFERENCE   3  (bases 1 to 7655)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7655)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Vector NTI Advanced v. 10
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (25-NOV-2013) Laboratory of Molecular Bacteriology, Medical
            University of Gdansk, Debinki 1, Gdansk 80-211, Poland"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7655
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             247..879
                     /codon_start=1
                     /gene="thrC"
                     /product="ThrC"
                     /label="thrC"
                     /note="N terminal part of thrC"
                     /protein_id="AHG54660.1"
                     /translation="MWKGLIHQYKEFLPVTDQTPALTLHEGNTPLIHLPKLSEQLGIEL
                     HVKTEGVNPTGSFKDRGMVMAVAKAKEEGNDTIMCASTGNTSAAAAAYAARANMKCIVI
                     IPNGKIAFGKLAQAVMYGAEIIAIDGNFDDALKIVRSICEKSPIALVNSVNPYRIEGQK
                     TAAFEIVHNGFPYAKYGQTWPARLLLFLTPDQLVMVATGAQLELRRY"
     gene            247..879
                     /gene="thrC"
                     /label="thrC"
     gene            complement(1124..2316)
                     /gene="cotZ"
                     /label="cotZ"
     misc_feature    complement(1124..1149)
                     /gene="cotZ"
                     /label="multiple cloning site"
                     /note="multiple cloning site"
     CDS             complement(1135..1626)
                     /codon_start=1
                     /gene="cotZ"
                     /product="CotZ"
                     /label="cotZ"
                     /note="spore coat protein"
                     /protein_id="AHG54656.1"
                     /translation="MSQKTSSCVREAVENIEDLQNAVEEDCPTGCHSKLLSVSHSLGDT
                     VPFAIFTSKSTPLVAFGNVGELDNGPCFNTVFFRVERVHGSCATLSLLIAFDEHKHILD
                     FTDKDTVCEVFRLEKTNYCIEVDLDCFCAINCLNPRLINRTHHHGGGEAAAKGGGGSGR
                     "
     regulatory      complement(1633..1638)
                     /gene="cotZ"
                     /regulatory_class="ribosome_binding_site"
     CDS             complement(1754..2293)
                     /codon_start=1
                     /gene="cotY"
                     /product="CotY"
                     /label="cotY"
                     /note="spore coat protein"
                     /protein_id="AHG54655.1"
                     /translation="MLLRTKVKYPIKKELKSMSCGKTHGRHENCVCDAVEKILAEQEAV
                     EEQCPTGCYTNLLNPTIAGKDTIPFLVFDKKGGLFSTFGNVGGFVDDMQCFESIFFRVE
                     KLCDCCATLSILRPVDVKGDTLSVCHPCDPDFFGLEKTDFCIEVDLGCFCAIQCLSPEL
                     VDRTSPHKDKKHHHNG"
     gene            complement(1754..2293)
                     /gene="cotY"
                     /label="cotY"
     regulatory      complement(2288..2316)
                     /gene="cotZ"
                     /regulatory_class="promoter"
     CDS             2536..3288
                     /codon_start=1
                     /gene="trpC"
                     /product="TrpC"
                     /label="trpC"
                     /protein_id="AHG54661.1"
                     /translation="MLEKIIKQKKEEVKTLVLPVEQPFEKRSFKEAPASPNRFIGLIAE
                     VKKASPSKGLIKEDFVPVQIAKDYEAAKADAISVLTDTPFFQGENSYLSDVKRAVSIPV
                     LRKDFIIDSLQVEESRRIGADAILLIGEVLDPLHLHELYLEAGEKGMDVLVEVHDASTL
                     EQILKVFTPDILGVNNRNLKTFETSVKQTEQIASLVPKESLLVSESGIGSLEHLTFVNE
                     HGARAVLIGESLMRQTSQRKAIHALFRE"
     gene            2536..3288
                     /gene="trpC"
                     /label="trpC"
     CDS             3718..4146
                     /codon_start=1
                     /gene="thrC"
                     /product="ThrC"
                     /label="thrC"
                     /note="C-terminal part of thrC"
                     /protein_id="AHG54659.1"
                     /translation="MRGFEAEGAAAIVRNEVIENPETIATAIRIGNPASWDKAVKAAEE
                     SNGKIDEVTDDEILHAYQLIARVEGVFAEPGSCASIAGVLKQVKSGEIPKGSKVVAVLT
                     GNGLKDPNTAVDISEIKPVTLPTDEDSILEYVKGAARV"
     gene            3718..4146
                     /gene="thrC"
                     /label="thrC"
     CDS             4143..4553
                     /codon_start=1
                     /gene="thrB"
                     /product="ThrB"
                     /label="thrB"
                     /note="N terminal part of thrB"
                     /protein_id="AHG54658.1"
                     /translation="MNEADMLFSVTVPGSTANLGPGFDSVGMALSRYLKLTVFESDKWS
                     FEAETETVAGINSSSFNFLTSSCIGTISRSSNPSSFTSIRFNQHSKNLTGHRQFFEQCA
                     NYQLFLQFIQGTGEPACNPSCSIMRNDPHPVS"
     gene            4143..4553
                     /gene="thrB"
                     /label="thrB"
     CDS             4797..5576
                     /gene="ant1"
                     /label="Spectinomycin 9-adenylyltransferase"
                     /note="Spectinomycin 9-adenylyltransferase from
                     Staphylococcus aureus (strain N315). Accession#: P0A0D1"
     rep_origin      complement(5826..6414)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(6588..7445)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(7446..7550)
                     /label="AmpR promoter"