LOCUS       V008272                 7655 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008272
VERSION     V008272
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7655)
  AUTHORS   Iwanicki A, Hinc K, Stasilojc M, Piatek I, Grela A, Lega T,
            Obuchowski M.
  TITLE     Vector system for spore sufrace display
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7655)
  AUTHORS   Iwanicki A, Hinc K, Stasilojc M, Piatek I, Grela A, Lega T,
            Obuchowski M.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2013) Laboratory of Molecular Bacteriology,
            Medical University of Gdansk, Debinki 1, Gdansk 80-211, Poland
REFERENCE   3  (bases 1 to 7655)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7655)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Vector NTI Advanced v. 10
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (25-NOV-2013) Laboratory of Molecular Bacteriology, Medical
            University of Gdansk, Debinki 1, Gdansk 80-211, Poland"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7655
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             247..879
                     /codon_start=1
                     /gene="thrC"
                     /product="ThrC"
                     /label="thrC"
                     /note="N terminal part of thrC"
                     /protein_id="AHG54660.1"
                     /translation="MWKGLIHQYKEFLPVTDQTPALTLHEGNTPLIHLPKLSEQLGIEL
                     HVKTEGVNPTGSFKDRGMVMAVAKAKEEGNDTIMCASTGNTSAAAAAYAARANMKCIVI
                     IPNGKIAFGKLAQAVMYGAEIIAIDGNFDDALKIVRSICEKSPIALVNSVNPYRIEGQK
                     TAAFEIVHNGFPYAKYGQTWPARLLLFLTPDQLVMVATGAQLELRRY"
     gene            247..879
                     /gene="thrC"
                     /label="thrC"
     gene            complement(1124..2316)
                     /gene="cotZ"
                     /label="cotZ"
     misc_feature    complement(1124..1149)
                     /gene="cotZ"
                     /label="multiple cloning site"
                     /note="multiple cloning site"
     CDS             complement(1135..1626)
                     /codon_start=1
                     /gene="cotZ"
                     /product="CotZ"
                     /label="cotZ"
                     /note="spore coat protein"
                     /protein_id="AHG54656.1"
                     /translation="MSQKTSSCVREAVENIEDLQNAVEEDCPTGCHSKLLSVSHSLGDT
                     VPFAIFTSKSTPLVAFGNVGELDNGPCFNTVFFRVERVHGSCATLSLLIAFDEHKHILD
                     FTDKDTVCEVFRLEKTNYCIEVDLDCFCAINCLNPRLINRTHHHGGGEAAAKGGGGSGR
                     "
     regulatory      complement(1633..1638)
                     /gene="cotZ"
                     /regulatory_class="ribosome_binding_site"
     CDS             complement(1754..2293)
                     /codon_start=1
                     /gene="cotY"
                     /product="CotY"
                     /label="cotY"
                     /note="spore coat protein"
                     /protein_id="AHG54655.1"
                     /translation="MLLRTKVKYPIKKELKSMSCGKTHGRHENCVCDAVEKILAEQEAV
                     EEQCPTGCYTNLLNPTIAGKDTIPFLVFDKKGGLFSTFGNVGGFVDDMQCFESIFFRVE
                     KLCDCCATLSILRPVDVKGDTLSVCHPCDPDFFGLEKTDFCIEVDLGCFCAIQCLSPEL
                     VDRTSPHKDKKHHHNG"
     gene            complement(1754..2293)
                     /gene="cotY"
                     /label="cotY"
     regulatory      complement(2288..2316)
                     /gene="cotZ"
                     /regulatory_class="promoter"
     CDS             2536..3288
                     /codon_start=1
                     /gene="trpC"
                     /product="TrpC"
                     /label="trpC"
                     /protein_id="AHG54661.1"
                     /translation="MLEKIIKQKKEEVKTLVLPVEQPFEKRSFKEAPASPNRFIGLIAE
                     VKKASPSKGLIKEDFVPVQIAKDYEAAKADAISVLTDTPFFQGENSYLSDVKRAVSIPV
                     LRKDFIIDSLQVEESRRIGADAILLIGEVLDPLHLHELYLEAGEKGMDVLVEVHDASTL
                     EQILKVFTPDILGVNNRNLKTFETSVKQTEQIASLVPKESLLVSESGIGSLEHLTFVNE
                     HGARAVLIGESLMRQTSQRKAIHALFRE"
     gene            2536..3288
                     /gene="trpC"
                     /label="trpC"
     CDS             3718..4146
                     /codon_start=1
                     /gene="thrC"
                     /product="ThrC"
                     /label="thrC"
                     /note="C-terminal part of thrC"
                     /protein_id="AHG54659.1"
                     /translation="MRGFEAEGAAAIVRNEVIENPETIATAIRIGNPASWDKAVKAAEE
                     SNGKIDEVTDDEILHAYQLIARVEGVFAEPGSCASIAGVLKQVKSGEIPKGSKVVAVLT
                     GNGLKDPNTAVDISEIKPVTLPTDEDSILEYVKGAARV"
     gene            3718..4146
                     /gene="thrC"
                     /label="thrC"
     CDS             4143..4553
                     /codon_start=1
                     /gene="thrB"
                     /product="ThrB"
                     /label="thrB"
                     /note="N terminal part of thrB"
                     /protein_id="AHG54658.1"
                     /translation="MNEADMLFSVTVPGSTANLGPGFDSVGMALSRYLKLTVFESDKWS
                     FEAETETVAGINSSSFNFLTSSCIGTISRSSNPSSFTSIRFNQHSKNLTGHRQFFEQCA
                     NYQLFLQFIQGTGEPACNPSCSIMRNDPHPVS"
     gene            4143..4553
                     /gene="thrB"
                     /label="thrB"
     CDS             4797..5576
                     /gene="ant1"
                     /label="Spectinomycin 9-adenylyltransferase"
                     /note="Spectinomycin 9-adenylyltransferase from
                     Staphylococcus aureus (strain N315). Accession#: P0A0D1"
     rep_origin      complement(5826..6414)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(6588..7445)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(7446..7550)
                     /label="AmpR promoter"
ORIGIN
        1 aattcatgta aaagatgagg ttggttcatt ctcgaaaatt acatctgtgt tctcagagcg
       61 gggcgtgacg tttgaaaaaa tccttcagct gccaattaaa ggccatgatg agttagctga
      121 aatcgtaatt gtcacacatc atacatcaga agctgatttc agtgatatcc tgcaaaacct
      181 aaatgatttg gaagtcgttc aagaagtcaa aagcacatat cgtgtagaag ggaacggttg
      241 gagctaatgt ggaaaggact tatccatcaa tataaagaat ttttacctgt aacagatcaa
      301 acaccggcgc taactttaca tgaaggaaac acacctctta ttcacctgcc gaagctgtct
      361 gagcagctcg gaattgagct tcatgtcaaa acggaaggcg tcaatcctac gggatcattt
      421 aaagatcgcg gaatggttat ggctgtggca aaggcaaaag aagaaggcaa tgacacgatt
      481 atgtgcgcgt caacaggtaa cacttccgct gctgcggcag catatgcagc ccgtgctaac
      541 atgaaatgca ttgtcatcat cccgaacgga aaaattgcat ttggaaaact cgctcaagct
      601 gtcatgtacg gagccgagat tatcgcaatt gacggaaact ttgacgatgc gcttaaaatt
      661 gtccgttcca tctgtgagaa atcaccgatt gcccttgtca actcagtcaa cccttaccgc
      721 attgaaggcc aaaaaactgc tgccttcgaa atagtacata atggatttcc ttacgcgaaa
      781 tacgggcaga catggcctgc ccggttatta ttatttttga caccagacca actggtaatg
      841 gtagcgaccg gcgctcagct ggaactccgc cgatactgac gggctccagg agtcgtcgcc
      901 accaatcccc atatggaaac cgtcgatatt cagccatgtg ccttcttccg cgtgcagcag
      961 atggcgatgg ctggtttcca tcagttgctg ttgactgtag cggctgatgt tgaactggaa
     1021 gtcgccgcgc cactggtgtg ggccataatt caattcgcgc gtcccgcagc gcagaccgtt
     1081 ttcgctcggg aagacgtacg gggtatacat gtaccggttg tacactagtg catgctagcg
     1141 gccggatcct cctccacctt tcgctgctgc ttctcctcca ccatgatgat gtgtacgatt
     1201 gattaatcga ggatttaagc agttgattgc gcagaagcag tctaagtcaa cttcaataca
     1261 gtagttcgtt ttttcgagtc ggaacacttc acacaccgta tctttatcgg tgaagtccaa
     1321 aatgtgtttg tgttcgtcaa atgcgattaa taatgacagt gttgcacagc ttccatgcac
     1381 tctttcgacc ctgaaaaata ctgtattaaa gcaagggccg ttatcgagtt cgccgacatt
     1441 tccgaaggcg actaatggcg ttgattttga tgtaaatatt gcaaaaggca ctgtgtcgcc
     1501 taacgaatgg cttacagata aaagcttaga gtggcagccg gtcgggcagt cttcttcaac
     1561 agcgttttgc agatcttcaa tattttctac agcttcacgc acgcagcttg atgttttctg
     1621 gctcatatta tccctcctgc cttataaatt tgagactggc gaaatgatgg agagtcagct
     1681 gcctcacgct ggaagcagct gaatgatttg cacctgtaat agagtttgga aggggattcc
     1741 tctttacaag tgtttatcca ttgtgatgat gctttttatc tttgtgaggc gatgttctgt
     1801 caactagctc tggtgacagg cactgaatcg cgcagaagca tccgagatcc acttcaatgc
     1861 agaaatctgt tttttctagc ccgaagaaat ccgggtcgca agggtggcaa acacttaagg
     1921 tatcgccttt gacatcgacc gggcgtaaaa tagacagtgt tgcacagcaa tcgcataatt
     1981 tttcgacgcg gaagaaaatg gattcaaagc attgcatatc atccacaaat ccccctacgt
     2041 ttccgaatgt ggagaacaat ccgccttttt tatcaaaaac gagaaacgga attgtgtctt
     2101 ttccagcaat cgtagggttt aaaaggttgg tatagcagcc agtcggacac tgttcttcaa
     2161 ctgcctcctg ctctgctaaa atcttttcca ctgcatcgca tacacagttc tcatgccggc
     2221 catgggtttt tccgcagctc attgatttca gctccttctt tatagggtat ttgactttag
     2281 tccttaacaa catatgtacc cgtgccccac ttggtgtggg tgaacgtcta tatattcatc
     2341 atatgcttgg aaaataagaa aaagcagagc gtttttagct ctgcttttag gtcctagagg
     2401 acaagagtga taactaggat ggctacgagt gtatttgttg ctgtagaaat aagggttgcg
     2461 aattcttttc aaaaaagtat tgacctagtt aactaaaaat gttactatta agtagtcgcc
     2521 aaggaggtga gacccatgct tgaaaaaatc atcaaacaaa agaaagaaga agtgaaaaca
     2581 ctggttctgc cggtagagca gcctttcgag aaacgttcat ttaaggaggc gccggcaagc
     2641 ccgaatcggt ttatcgggtt gattgccgaa gtgaagaaag catcgccgtc aaaagggctt
     2701 attaaagagg attttgtacc tgtgcagatt gcaaaagact atgaggctgc gaaggcagat
     2761 gcgatttccg ttttaacaga caccccgttt tttcaagggg aaaacagcta tttatcagac
     2821 gtaaagcgtg ctgtttcgat tcctgtactt agaaaagatt ttattattga ttctcttcaa
     2881 gtagaggaat caagaagaat cggagcggat gccatattgt taatcggcga ggtgcttgat
     2941 cccttacacc ttcatgaatt atatcttgaa gcaggtgaaa aggggatgga cgtgttagtg
     3001 gaggttcatg atgcatcaac gctagaacaa atattgaaag tgttcacacc cgacattctc
     3061 ggcgtaaata atcgaaacct aaaaacgttt gaaacatctg taaagcagac agaacaaatc
     3121 gcatctctcg ttccgaaaga atccttgctt gtcagcgaaa gcggaatcgg ttctttagaa
     3181 catttaacat ttgtcaatga acatggggcg cgagctgtac ttatcggtga atcattgatg
     3241 agacaaactt ctcagcgtaa agcaatccat gctttgttta gggagtaata cgtactttgt
     3301 ttcaaaatgg gtcaatcgag aatatcgtca actgtttact aaaaatcagt ttcatcaagc
     3361 aatgaaacac gccaaagtaa acaatttaag taccgttact tatgagcaag tattgtctat
     3421 ttttaatagt tatctattat ttaacgggag gaaataattc tatgagtcgc ttttgtaaat
     3481 ttggaaagtt acacgttact aaagggaatg tagataaatt attaggtata ctactgacag
     3541 cttccaagga gctaaagagg tccctagcgc ctacggggaa tttgtatcga agtgtgcgaa
     3601 cagctggggg aagcgcctga tgttttagcg atcccggtcg gaaatgctgg aaacatcact
     3661 gcgtactgga agggcttcaa ggaatatcat gagaaaaacg gcacaggcct tccgaaaatg
     3721 cgcggctttg aagctgaggg tgcggcggca atcgtgcgca atgaagtgat tgaaaatccg
     3781 gaaacaatag cgacagccat tcgtatcgga aacccggcaa gctgggacaa agctgtaaag
     3841 gcagccgagg aatccaatgg gaaaattgac gaagtcactg atgatgaaat ccttcacgca
     3901 tatcagctga tcgcccgtgt agaaggcgtg tttgcagaac caggttcttg cgcgtctatc
     3961 gcaggagtgc tgaaacaggt gaaatccgga gaaattccga aaggcagcaa ggtcgtagct
     4021 gtgttaacag gaaacggact gaaagatccg aacacagcgg tcgacatttc agaaatcaag
     4081 cctgtcacat tgccgactga tgaagacagc atccttgaat atgtaaaagg agcggcccgt
     4141 gtatgaacga agccgacatg ctgttctctg tcactgttcc cggaagcaca gctaacctag
     4201 gccccggctt tgattcagtc ggaatggcgc tcagcagata tttgaagctg accgtctttg
     4261 aaagcgacaa atggtctttt gaggctgaaa cagaaacagt cgccggaatt aattcgagct
     4321 cctttaactt cctcaccagt agttgtatcg gtaccataag tagaagcagc aacccaagta
     4381 gctttaccag catccggttc aaccagcata gtaagaatct tactggacat cggcagttct
     4441 tcgaacagtg cgccaactac cagctctttc tgcagttcat tcagggcacc ggagaacctg
     4501 cgtgcaatcc atcttgttca atcatgcgaa acgatcctca tcctgtctct tgatccatgg
     4561 attacgcgtt aacccgggcc cgcggatgca tatgatcaga tcttaaggcc taggtctaga
     4621 ggatcgatct gtataataaa gaataattat taatctgtag acaaattgtg aaaggatgta
     4681 cttaaacgct aacggtcagc tttattgaac agtaatttaa gtatatgtcc aatctagggt
     4741 aagtaaattg agtatcaata taaactttat atgaacataa tcaacgaggt gaaatcatga
     4801 gcaatttgat taacggaaaa ataccaaatc aagcgattca aacattaaaa atcgtaaaag
     4861 atttatttgg aagttcaata gttggagtat atctatttgg ttcagcagta aatggtggtt
     4921 tacgcattaa cagcgatgta gatgttctag tcgtcgtgaa tcatagttta cctcaattaa
     4981 ctcgaaaaaa actaacagaa agactaatga ctatatcagg aaagattgga aatacggatt
     5041 ctgttagacc acttgaagtt acggttataa ataggagtga agttgtccct tggcaatatc
     5101 ctccaaaaag agaatttata tacggtgagt ggctcagggg tgaatttgag aatggacaaa
     5161 ttcaggaacc aagctatgat cctgatttgg ctattgtttt agcacaagca agaaagaata
     5221 gtatttctct atttggtcct gattcttcaa gtatacttgt ctccgtacct ttgacagata
     5281 ttcgaagagc aattaaggat tctttgccag aactaattga ggggataaaa ggtgatgagc
     5341 gtaatgtaat tttaacccta gctcgaatgt ggcaaacagt gactactggt gaaattacct
     5401 cgaaagatgt cgctgcagaa tgggctatac ctcttttacc taaagagcat gtaactttac
     5461 tggatatagc tagaaaaggc tatcggggag agtgtgatga taagtgggaa ggactatatt
     5521 caaaggtgaa agcactcgtt aagtatatga aaaattctat agaaacttct ctcaattagg
     5581 ctaattttat tgcaataaca ggtgcttact tttctggagt tctttagcaa atttttttat
     5641 tagctgaact tagtattagt ggccatactc ctccaatcca aagctattta gaaagattac
     5701 tatatcctca aacaggcggt aaccggcctc ttcatcggga atgcgcgcga ccttcagcat
     5761 cgccggcatg tccccctggc ggacgggaag tatccagctc gaggtcgggc cgcgttgctg
     5821 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     5881 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     5941 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     6001 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     6061 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     6121 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     6181 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     6241 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
     6301 gttaccttcg gaaaaagagt tgatagctct tgatccggca aacaaaccac cgctggtagc
     6361 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     6421 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     6481 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     6541 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     6601 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     6661 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     6721 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     6781 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     6841 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     6901 gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     6961 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     7021 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     7081 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     7141 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     7201 acacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     7261 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     7321 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     7381 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     7441 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     7501 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     7561 cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat
     7621 aggcgtatca cgaggccctt tcgtcttcaa gaatt
//
