pCotZ-C-thr vector (V008273)

LOCUS       V008273                 7578 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008273
VERSION     V008273
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7578)
  AUTHORS   Iwanicki A, Hinc K, Stasilojc M, Piatek I, Grela A, Lega T,
            Obuchowski M.
  TITLE     Vector system for spore sufrace display
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7578)
  AUTHORS   Iwanicki A, Hinc K, Stasilojc M, Piatek I, Grela A, Lega T,
            Obuchowski M.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2013) Laboratory of Molecular Bacteriology,
            Medical University of Gdansk, Debinki 1, Gdansk 80-211, Poland
REFERENCE   3  (bases 1 to 7578)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7578)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Vector NTI Advanced v. 10
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (25-NOV-2013) Laboratory of Molecular Bacteriology, Medical
            University of Gdansk, Debinki 1, Gdansk 80-211, Poland"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7578
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             247..879
                     /codon_start=1
                     /gene="thrC"
                     /product="ThrC"
                     /label="thrC"
                     /note="N terminal part of thrC"
                     /protein_id="AHG54652.1"
                     /translation="MWKGLIHQYKEFLPVTDQTPALTLHEGNTPLIHLPKLSEQLGIEL
                     HVKTEGVNPTGSFKDRGMVMAVAKAKEEGNDTIMCASTGNTSAAAAAYAARANMKCIVI
                     IPNGKIAFGKLAQAVMYGAEIIAIDGNFDDALKIVRSICEKSPIALVNSVNPYRIEGQK
                     TAAFEIVHNGFPYAKYGQTWPARLLLFLTPDQLVMVATGAQLELRRY"
     gene            247..879
                     /gene="thrC"
                     /label="thrC"
     gene            complement(1135..2283)
                     /gene="cotZ"
                     /label="cotZ"
     CDS             complement(1135..1593)
                     /codon_start=1
                     /gene="cotZ"
                     /product="CotZ"
                     /label="cotZ"
                     /note="spore coat protein"
                     /protein_id="AHG54649.1"
                     /translation="MSQKTSSCVREAVENIEDLQNAVEEDCPTGCHSKLLSVSHSLGDT
                     VPFAIFTSKSTPLVAFGNVGELDNGPCFNTVFFRVERVHGSCATLSLLIAFDEHKHILD
                     FTDKDTVCEVFRLEKTNYCIEVDLDCFCAINCLNPRLINRTHHHGSGR"
     regulatory      complement(1600..1605)
                     /gene="cotZ"
                     /regulatory_class="ribosome_binding_site"
     CDS             complement(1721..2260)
                     /codon_start=1
                     /gene="cotY"
                     /product="CotY"
                     /label="cotY"
                     /note="spore coat protein"
                     /protein_id="AHG54648.1"
                     /translation="MLLRTKVKYPIKKELKSMSCGKTHGRHENCVCDAVEKILAEQEAV
                     EEQCPTGCYTNLLNPTIAGKDTIPFLVFDKKGGLFSTFGNVGGFVDDMQCFESIFFRVE
                     KLCDCCATLSILRPVDVKGDTLSVCHPCDPDFFGLEKTDFCIEVDLGCFCAIQCLSPEL
                     VDRTSPHKDKKHHHNG"
     gene            complement(1721..2260)
                     /gene="cotY"
                     /label="cotY"
     regulatory      complement(2255..2263)
                     /gene="cotZ"
                     /regulatory_class="minus_10_signal"
     regulatory      complement(2280..2283)
                     /gene="cotZ"
                     /regulatory_class="minus_35_signal"
     CDS             2459..3211
                     /codon_start=1
                     /gene="trpC"
                     /product="TrpC"
                     /label="trpC"
                     /protein_id="AHG54653.1"
                     /translation="MLEKIIKQKKEEVKTLVLPVEQPFEKRSFKEAPASPNRFIGLIAE
                     VKKASPSKGLIKEDFVPVQIAKDYEAAKADAISVLTDTPFFQGENSYLSDVKRAVSIPV
                     LRKDFIIDSLQVEESRRIGADAILLIGEVLDPLHLHELYLEAGEKGMDVLVEVHDASTL
                     EQILKVFTPDILGVNNRNLKTFETSVKQTEQIASLVPKESLLVSESGIGSLEHLTFVNE
                     HGARAVLIGESLMRQTSQRKAIHALFRE"
     gene            2459..3211
                     /gene="trpC"
                     /label="trpC"
     CDS             3641..4069
                     /codon_start=1
                     /gene="thrC"
                     /product="ThrC"
                     /label="thrC"
                     /note="C-terminal part of thrC"
                     /protein_id="AHG54651.1"
                     /translation="MRGFEAEGAAAIVRNEVIENPETIATAIRIGNPASWDKAVKAAEE
                     SNGKIDEVTDDEILHAYQLIARVEGVFAEPGSCASIAGVLKQVKSGEIPKGSKVVAVLT
                     GNGLKDPNTAVDISEIKPVTLPTDEDSILEYVKGAARV"
     gene            3641..4069
                     /gene="thrC"
                     /label="thrC"
     CDS             4066..4476
                     /codon_start=1
                     /gene="thrB"
                     /product="ThrB"
                     /label="thrB"
                     /note="N terminal part of thrB"
                     /protein_id="AHG54650.1"
                     /translation="MNEADMLFSVTVPGSTANLGPGFDSVGMALSRYLKLTVFESDKWS
                     FEAETETVAGINSSSFNFLTSSCIGTISRSSNPSSFTSIRFNQHSKNLTGHRQFFEQCA
                     NYQLFLQFIQGTGEPACNPSCSIMRNDPHPVS"
     gene            4066..4476
                     /gene="thrB"
                     /label="thrB"
     CDS             4720..5499
                     /gene="ant1"
                     /label="Spectinomycin 9-adenylyltransferase"
                     /note="Spectinomycin 9-adenylyltransferase from
                     Staphylococcus aureus (strain N315). Accession#: P0A0D1"
     rep_origin      complement(5749..6337)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(6511..7368)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(7369..7473)
                     /label="AmpR promoter"