LOCUS       V008273                 7578 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008273
VERSION     V008273
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7578)
  AUTHORS   Iwanicki A, Hinc K, Stasilojc M, Piatek I, Grela A, Lega T,
            Obuchowski M.
  TITLE     Vector system for spore sufrace display
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7578)
  AUTHORS   Iwanicki A, Hinc K, Stasilojc M, Piatek I, Grela A, Lega T,
            Obuchowski M.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2013) Laboratory of Molecular Bacteriology,
            Medical University of Gdansk, Debinki 1, Gdansk 80-211, Poland
REFERENCE   3  (bases 1 to 7578)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7578)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Vector NTI Advanced v. 10
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (25-NOV-2013) Laboratory of Molecular Bacteriology, Medical
            University of Gdansk, Debinki 1, Gdansk 80-211, Poland"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7578
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             247..879
                     /codon_start=1
                     /gene="thrC"
                     /product="ThrC"
                     /label="thrC"
                     /note="N terminal part of thrC"
                     /protein_id="AHG54652.1"
                     /translation="MWKGLIHQYKEFLPVTDQTPALTLHEGNTPLIHLPKLSEQLGIEL
                     HVKTEGVNPTGSFKDRGMVMAVAKAKEEGNDTIMCASTGNTSAAAAAYAARANMKCIVI
                     IPNGKIAFGKLAQAVMYGAEIIAIDGNFDDALKIVRSICEKSPIALVNSVNPYRIEGQK
                     TAAFEIVHNGFPYAKYGQTWPARLLLFLTPDQLVMVATGAQLELRRY"
     gene            247..879
                     /gene="thrC"
                     /label="thrC"
     gene            complement(1135..2283)
                     /gene="cotZ"
                     /label="cotZ"
     CDS             complement(1135..1593)
                     /codon_start=1
                     /gene="cotZ"
                     /product="CotZ"
                     /label="cotZ"
                     /note="spore coat protein"
                     /protein_id="AHG54649.1"
                     /translation="MSQKTSSCVREAVENIEDLQNAVEEDCPTGCHSKLLSVSHSLGDT
                     VPFAIFTSKSTPLVAFGNVGELDNGPCFNTVFFRVERVHGSCATLSLLIAFDEHKHILD
                     FTDKDTVCEVFRLEKTNYCIEVDLDCFCAINCLNPRLINRTHHHGSGR"
     regulatory      complement(1600..1605)
                     /gene="cotZ"
                     /regulatory_class="ribosome_binding_site"
     CDS             complement(1721..2260)
                     /codon_start=1
                     /gene="cotY"
                     /product="CotY"
                     /label="cotY"
                     /note="spore coat protein"
                     /protein_id="AHG54648.1"
                     /translation="MLLRTKVKYPIKKELKSMSCGKTHGRHENCVCDAVEKILAEQEAV
                     EEQCPTGCYTNLLNPTIAGKDTIPFLVFDKKGGLFSTFGNVGGFVDDMQCFESIFFRVE
                     KLCDCCATLSILRPVDVKGDTLSVCHPCDPDFFGLEKTDFCIEVDLGCFCAIQCLSPEL
                     VDRTSPHKDKKHHHNG"
     gene            complement(1721..2260)
                     /gene="cotY"
                     /label="cotY"
     regulatory      complement(2255..2263)
                     /gene="cotZ"
                     /regulatory_class="minus_10_signal"
     regulatory      complement(2280..2283)
                     /gene="cotZ"
                     /regulatory_class="minus_35_signal"
     CDS             2459..3211
                     /codon_start=1
                     /gene="trpC"
                     /product="TrpC"
                     /label="trpC"
                     /protein_id="AHG54653.1"
                     /translation="MLEKIIKQKKEEVKTLVLPVEQPFEKRSFKEAPASPNRFIGLIAE
                     VKKASPSKGLIKEDFVPVQIAKDYEAAKADAISVLTDTPFFQGENSYLSDVKRAVSIPV
                     LRKDFIIDSLQVEESRRIGADAILLIGEVLDPLHLHELYLEAGEKGMDVLVEVHDASTL
                     EQILKVFTPDILGVNNRNLKTFETSVKQTEQIASLVPKESLLVSESGIGSLEHLTFVNE
                     HGARAVLIGESLMRQTSQRKAIHALFRE"
     gene            2459..3211
                     /gene="trpC"
                     /label="trpC"
     CDS             3641..4069
                     /codon_start=1
                     /gene="thrC"
                     /product="ThrC"
                     /label="thrC"
                     /note="C-terminal part of thrC"
                     /protein_id="AHG54651.1"
                     /translation="MRGFEAEGAAAIVRNEVIENPETIATAIRIGNPASWDKAVKAAEE
                     SNGKIDEVTDDEILHAYQLIARVEGVFAEPGSCASIAGVLKQVKSGEIPKGSKVVAVLT
                     GNGLKDPNTAVDISEIKPVTLPTDEDSILEYVKGAARV"
     gene            3641..4069
                     /gene="thrC"
                     /label="thrC"
     CDS             4066..4476
                     /codon_start=1
                     /gene="thrB"
                     /product="ThrB"
                     /label="thrB"
                     /note="N terminal part of thrB"
                     /protein_id="AHG54650.1"
                     /translation="MNEADMLFSVTVPGSTANLGPGFDSVGMALSRYLKLTVFESDKWS
                     FEAETETVAGINSSSFNFLTSSCIGTISRSSNPSSFTSIRFNQHSKNLTGHRQFFEQCA
                     NYQLFLQFIQGTGEPACNPSCSIMRNDPHPVS"
     gene            4066..4476
                     /gene="thrB"
                     /label="thrB"
     CDS             4720..5499
                     /gene="ant1"
                     /label="Spectinomycin 9-adenylyltransferase"
                     /note="Spectinomycin 9-adenylyltransferase from
                     Staphylococcus aureus (strain N315). Accession#: P0A0D1"
     rep_origin      complement(5749..6337)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(6511..7368)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(7369..7473)
                     /label="AmpR promoter"
ORIGIN
        1 aattcatgta aaagatgagg ttggttcatt ctcgaaaatt acatctgtgt tctcagagcg
       61 gggcgtgacg tttgaaaaaa tccttcagct gccaattaaa ggccatgatg agttagctga
      121 aatcgtaatt gtcacacatc atacatcaga agctgatttc agtgatatcc tgcaaaacct
      181 aaatgatttg gaagtcgttc aagaagtcaa aagcacatat cgtgtagaag ggaacggttg
      241 gagctaatgt ggaaaggact tatccatcaa tataaagaat ttttacctgt aacagatcaa
      301 acaccggcgc taactttaca tgaaggaaac acacctctta ttcacctgcc gaagctgtct
      361 gagcagctcg gaattgagct tcatgtcaaa acggaaggcg tcaatcctac gggatcattt
      421 aaagatcgcg gaatggttat ggctgtggca aaggcaaaag aagaaggcaa tgacacgatt
      481 atgtgcgcgt caacaggtaa cacttccgct gctgcggcag catatgcagc ccgtgctaac
      541 atgaaatgca ttgtcatcat cccgaacgga aaaattgcat ttggaaaact cgctcaagct
      601 gtcatgtacg gagccgagat tatcgcaatt gacggaaact ttgacgatgc gcttaaaatt
      661 gtccgttcca tctgtgagaa atcaccgatt gcccttgtca actcagtcaa cccttaccgc
      721 attgaaggcc aaaaaactgc tgccttcgaa atagtacata atggatttcc ttacgcgaaa
      781 tacgggcaga catggcctgc ccggttatta ttatttttga caccagacca actggtaatg
      841 gtagcgaccg gcgctcagct ggaactccgc cgatactgac gggctccagg agtcgtcgcc
      901 accaatcccc atatggaaac cgtcgatatt cagccatgtg ccttcttccg cgtgcagcag
      961 atggcgatgg ctggtttcca tcagttgctg ttgactgtag cggctgatgt tgaactggaa
     1021 gtcgccgcgc cactggtgtg ggccataatt caattcgcgc gtcccgcagc gcagaccgtt
     1081 ttcgctcggg aagacgtacg gggtatacat gtaccggttg tacactagtg catgctagcg
     1141 gccggatcca tgatgatgtg tacgattgat taatcgagga tttaagcagt tgattgcgca
     1201 gaagcagtct aagtcaactt caatacagta gttcgttttt tcgagtcgga acacttcaca
     1261 caccgtatct ttatcggtga agtccaaaat gtgtttgtgt tcgtcaaatg cgattaataa
     1321 tgacagtgtt gcacagcttc catgcactct ttcgaccctg aaaaatactg tattaaagca
     1381 agggccgtta tcgagttcgc cgacatttcc gaaggcgact aatggcgttg attttgatgt
     1441 aaatattgca aaaggcactg tgtcgcctaa cgaatggctt acagataaaa gcttagagtg
     1501 gcagccggtc gggcagtctt cttcaacagc gttttgcaga tcttcaatat tttctacagc
     1561 ttcacgcacg cagcttgatg ttttctggct catattatcc ctcctgcctt ataaatttga
     1621 gactggcgaa atgatggaga gtcagctgcc tcacgctgga agcagctgaa tgatttgcac
     1681 ctgtaataga gtttggaagg ggattcctct ttacaagtgt ttatccattg tgatgatgct
     1741 ttttatcttt gtgaggcgat gttctgtcaa ctagctctgg tgacaggcac tgaatcgcgc
     1801 agaagcatcc gagatccact tcaatgcaga aatctgtttt ttctagcccg aagaaatccg
     1861 ggtcgcaagg gtggcaaaca cttaaggtat cgcctttgac atcgaccggg cgtaaaatag
     1921 acagtgttgc acagcaatcg cataattttt cgacgcggaa gaaaatggat tcaaagcatt
     1981 gcatatcatc cacaaatccc cctacgtttc cgaatgtgga gaacaatccg ccttttttat
     2041 caaaaacgag aaacggaatt gtgtcttttc cagcaatcgt agggtttaaa aggttggtat
     2101 agcagccagt cggacactgt tcttcaactg cctcctgctc tgctaaaatc ttttccactg
     2161 catcgcatac acagttctca tgccggccat gggtttttcc gcagctcatt gatttcagct
     2221 ccttctttat agggtatttg actttagtcc ttaacaacat atgtacccgt gccccacttg
     2281 gtgtgggtga acgtctatat attcatcata tgcttggaaa ataagaaaaa gcagagcgtt
     2341 tttagctctg cttttaggtc ctagaggaca agagtgataa ctgaattctt ttcaaaaaag
     2401 tattgaccta gttaactaaa aatgttacta ttaagtagtc gccaaggagg tgagacccat
     2461 gcttgaaaaa atcatcaaac aaaagaaaga agaagtgaaa acactggttc tgccggtaga
     2521 gcagcctttc gagaaacgtt catttaagga ggcgccggca agcccgaatc ggtttatcgg
     2581 gttgattgcc gaagtgaaga aagcatcgcc gtcaaaaggg cttattaaag aggattttgt
     2641 acctgtgcag attgcaaaag actatgaggc tgcgaaggca gatgcgattt ccgttttaac
     2701 agacaccccg ttttttcaag gggaaaacag ctatttatca gacgtaaagc gtgctgtttc
     2761 gattcctgta cttagaaaag attttattat tgattctctt caagtagagg aatcaagaag
     2821 aatcggagcg gatgccatat tgttaatcgg cgaggtgctt gatcccttac accttcatga
     2881 attatatctt gaagcaggtg aaaaggggat ggacgtgtta gtggaggttc atgatgcatc
     2941 aacgctagaa caaatattga aagtgttcac acccgacatt ctcggcgtaa ataatcgaaa
     3001 cctaaaaacg tttgaaacat ctgtaaagca gacagaacaa atcgcatctc tcgttccgaa
     3061 agaatccttg cttgtcagcg aaagcggaat cggttcttta gaacatttaa catttgtcaa
     3121 tgaacatggg gcgcgagctg tacttatcgg tgaatcattg atgagacaaa cttctcagcg
     3181 taaagcaatc catgctttgt ttagggagta atacgtactt tgtttcaaaa tgggtcaatc
     3241 gagaatatcg tcaactgttt actaaaaatc agtttcatca agcaatgaaa cacgccaaag
     3301 taaacaattt aagtaccgtt acttatgagc aagtattgtc tatttttaat agttatctat
     3361 tatttaacgg gaggaaataa ttctatgagt cgcttttgta aatttggaaa gttacacgtt
     3421 actaaaggga atgtagataa attattaggt atactactga cagcttccaa ggagctaaag
     3481 aggtccctag cgcctacggg gaatttgtat cgaagtgtgc gaacagctgg gggaagcgcc
     3541 tgatgtttta gcgatcccgg tcggaaatgc tggaaacatc actgcgtact ggaagggctt
     3601 caaggaatat catgagaaaa acggcacagg ccttccgaaa atgcgcggct ttgaagctga
     3661 gggtgcggcg gcaatcgtgc gcaatgaagt gattgaaaat ccggaaacaa tagcgacagc
     3721 cattcgtatc ggaaacccgg caagctggga caaagctgta aaggcagccg aggaatccaa
     3781 tgggaaaatt gacgaagtca ctgatgatga aatccttcac gcatatcagc tgatcgcccg
     3841 tgtagaaggc gtgtttgcag aaccaggttc ttgcgcgtct atcgcaggag tgctgaaaca
     3901 ggtgaaatcc ggagaaattc cgaaaggcag caaggtcgta gctgtgttaa caggaaacgg
     3961 actgaaagat ccgaacacag cggtcgacat ttcagaaatc aagcctgtca cattgccgac
     4021 tgatgaagac agcatccttg aatatgtaaa aggagcggcc cgtgtatgaa cgaagccgac
     4081 atgctgttct ctgtcactgt tcccggaagc acagctaacc taggccccgg ctttgattca
     4141 gtcggaatgg cgctcagcag atatttgaag ctgaccgtct ttgaaagcga caaatggtct
     4201 tttgaggctg aaacagaaac agtcgccgga attaattcga gctcctttaa cttcctcacc
     4261 agtagttgta tcggtaccat aagtagaagc agcaacccaa gtagctttac cagcatccgg
     4321 ttcaaccagc atagtaagaa tcttactgga catcggcagt tcttcgaaca gtgcgccaac
     4381 taccagctct ttctgcagtt cattcagggc accggagaac ctgcgtgcaa tccatcttgt
     4441 tcaatcatgc gaaacgatcc tcatcctgtc tcttgatcca tggattacgc gttaacccgg
     4501 gcccgcggat gcatatgatc agatcttaag gcctaggtct agaggatcga tctgtataat
     4561 aaagaataat tattaatctg tagacaaatt gtgaaaggat gtacttaaac gctaacggtc
     4621 agctttattg aacagtaatt taagtatatg tccaatctag ggtaagtaaa ttgagtatca
     4681 atataaactt tatatgaaca taatcaacga ggtgaaatca tgagcaattt gattaacgga
     4741 aaaataccaa atcaagcgat tcaaacatta aaaatcgtaa aagatttatt tggaagttca
     4801 atagttggag tatatctatt tggttcagca gtaaatggtg gtttacgcat taacagcgat
     4861 gtagatgttc tagtcgtcgt gaatcatagt ttacctcaat taactcgaaa aaaactaaca
     4921 gaaagactaa tgactatatc aggaaagatt ggaaatacgg attctgttag accacttgaa
     4981 gttacggtta taaataggag tgaagttgtc ccttggcaat atcctccaaa aagagaattt
     5041 atatacggtg agtggctcag gggtgaattt gagaatggac aaattcagga accaagctat
     5101 gatcctgatt tggctattgt tttagcacaa gcaagaaaga atagtatttc tctatttggt
     5161 cctgattctt caagtatact tgtctccgta cctttgacag atattcgaag agcaattaag
     5221 gattctttgc cagaactaat tgaggggata aaaggtgatg agcgtaatgt aattttaacc
     5281 ctagctcgaa tgtggcaaac agtgactact ggtgaaatta cctcgaaaga tgtcgctgca
     5341 gaatgggcta tacctctttt acctaaagag catgtaactt tactggatat agctagaaaa
     5401 ggctatcggg gagagtgtga tgataagtgg gaaggactat attcaaaggt gaaagcactc
     5461 gttaagtata tgaaaaattc tatagaaact tctctcaatt aggctaattt tattgcaata
     5521 acaggtgctt acttttctgg agttctttag caaatttttt tattagctga acttagtatt
     5581 agtggccata ctcctccaat ccaaagctat ttagaaagat tactatatcc tcaaacaggc
     5641 ggtaaccggc ctcttcatcg ggaatgcgcg cgaccttcag catcgccggc atgtccccct
     5701 ggcggacggg aagtatccag ctcgaggtcg ggccgcgttg ctggcgtttt tccataggct
     5761 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     5821 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     5881 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     5941 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     6001 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     6061 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     6121 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     6181 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     6241 agttgatagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     6301 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     6361 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     6421 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     6481 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     6541 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     6601 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     6661 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     6721 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     6781 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc
     6841 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     6901 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     6961 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     7021 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     7081 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaacacggg ataataccgc
     7141 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     7201 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     7261 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     7321 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     7381 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     7441 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     7501 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
     7561 ctttcgtctt caagaatt
//
