pCotC-N vector (V008279)

LOCUS       V008279                 5465 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008279
VERSION     V008279
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5465)
  AUTHORS   Iwanicki A, Hinc K, Stasilojc M, Piatek I, Grela A, Lega T,
            Obuchowski M.
  TITLE     Vector system for spore sufrace display
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5465)
  AUTHORS   Iwanicki A, Hinc K, Stasilojc M, Piatek I, Grela A, Lega T,
            Obuchowski M.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-OCT-2013) Laboratory of Molecular Bacteriology,
            Medical University of Gdansk, Debinki 1, Gdansk 80-211, Poland
REFERENCE   3  (bases 1 to 5465)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5465)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Vectro NTI Advanced v. 10
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (24-OCT-2013) Laboratory of Molecular Bacteriology, Medical
            University of Gdansk, Debinki 1, Gdansk 80-211, Poland"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5465
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_recomb     complement(401..873)
                     /label="C-terminal part of lacA"
                     /note="C-terminal part of lacA"
     gene            986..1268
                     /gene="cotC"
                     /label="cotC"
     regulatory      986..1032
                     /gene="cotC"
                     /regulatory_class="promoter"
     regulatory      1040..1045
                     /gene="cotC"
                     /regulatory_class="ribosome_binding_site"
     CDS             1053..1268
                     /codon_start=1
                     /gene="cotC"
                     /product="CotC"
                     /label="cotC"
                     /note="spore coat protein"
                     /protein_id="AHG54607.1"
                     /translation="MGTRGSGYYKKYKEEYYTVKKTYYKKYYEYDKKDYDCDYDKKYDD
                     YDKKYYDHDKKDYDYVVEYKKHKKHY"
     misc_feature    1056..1070
                     /gene="cotC"
                     /label="multiple cloning site"
                     /note="multiple cloning site"
     regulatory      1333..1518
                     /gene="hisD"
                     /label="promoter region"
                     /note="promoter region"
                     /regulatory_class="promoter"
     CDS             1545..2825
                     /gene="hisD"
                     /label="Histidinol dehydrogenase"
                     /note="Histidinol dehydrogenase from Bacillus subtilis
                     (strain 168). Accession#: O34651"
     misc_recomb     complement(2847..3225)
                     /label="N terminal part of lacA"
                     /note="N terminal part of lacA"
     primer_bind     complement(3244..3260)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(3268..3284)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3292..3322)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3337..3358)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3646..4234)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(4408..5265)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(5266..5370)
                     /label="AmpR promoter"