LOCUS       V008279                 5465 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008279
VERSION     V008279
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5465)
  AUTHORS   Iwanicki A, Hinc K, Stasilojc M, Piatek I, Grela A, Lega T,
            Obuchowski M.
  TITLE     Vector system for spore sufrace display
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5465)
  AUTHORS   Iwanicki A, Hinc K, Stasilojc M, Piatek I, Grela A, Lega T,
            Obuchowski M.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-OCT-2013) Laboratory of Molecular Bacteriology,
            Medical University of Gdansk, Debinki 1, Gdansk 80-211, Poland
REFERENCE   3  (bases 1 to 5465)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5465)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Vectro NTI Advanced v. 10
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (24-OCT-2013) Laboratory of Molecular Bacteriology, Medical
            University of Gdansk, Debinki 1, Gdansk 80-211, Poland"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5465
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_recomb     complement(401..873)
                     /label="C-terminal part of lacA"
                     /note="C-terminal part of lacA"
     gene            986..1268
                     /gene="cotC"
                     /label="cotC"
     regulatory      986..1032
                     /gene="cotC"
                     /regulatory_class="promoter"
     regulatory      1040..1045
                     /gene="cotC"
                     /regulatory_class="ribosome_binding_site"
     CDS             1053..1268
                     /codon_start=1
                     /gene="cotC"
                     /product="CotC"
                     /label="cotC"
                     /note="spore coat protein"
                     /protein_id="AHG54607.1"
                     /translation="MGTRGSGYYKKYKEEYYTVKKTYYKKYYEYDKKDYDCDYDKKYDD
                     YDKKYYDHDKKDYDYVVEYKKHKKHY"
     misc_feature    1056..1070
                     /gene="cotC"
                     /label="multiple cloning site"
                     /note="multiple cloning site"
     regulatory      1333..1518
                     /gene="hisD"
                     /label="promoter region"
                     /note="promoter region"
                     /regulatory_class="promoter"
     CDS             1545..2825
                     /gene="hisD"
                     /label="Histidinol dehydrogenase"
                     /note="Histidinol dehydrogenase from Bacillus subtilis
                     (strain 168). Accession#: O34651"
     misc_recomb     complement(2847..3225)
                     /label="N terminal part of lacA"
                     /note="N terminal part of lacA"
     primer_bind     complement(3244..3260)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(3268..3284)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3292..3322)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3337..3358)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3646..4234)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(4408..5265)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(5266..5370)
                     /label="AmpR promoter"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cctgatcctg cctcgcttgt
      421 acggagacgc cttttccgtg ccgaaccgga aaaactggag agagagacag gtctgtgatc
      481 agaccctcat agaaatcacg ctgaaattga tcctccaaac gcgcgccgat aaaatacgcc
      541 ttgccctgct gatactcatg gcttgtgacc gctggcgtgc gcgcataaaa atcttcttga
      601 tacaccgctt ccactgaagc tgtctttaca tcaatcacgg ttgcataatc cttcatttca
      661 tatatttggc tgcggtagct gacagcgttt cgatccttcg gatacagggt gtccgtttca
      721 agaggctcaa ctccaaatat agcttgaaga tccggatgcc atccgcctgt gtatgttaag
      781 tcatgctcat tcacaacccc gctgatatac gtcatgacta aggtgccgcc gtcagccgta
      841 aacgctttta aacgggaaac ggtgtcctcg ctggagctca ggataaatcg tttgggccga
      901 tgaaaaatcg gctctttatt ttgatttgtt tttgtgtcat ctgtcttttt ctatcatttg
      961 gacagccctt ttttccttct atgattttag tccaagccgc aaaatctact cgccgtataa
     1021 taaagcgtag taaaaataaa ggaggagtat atatgggtac ccggggatcc ggttattaca
     1081 aaaaatacaa agaagagtat tatacggtca aaaaaacgta ttataagaag tattacgaat
     1141 atgataaaaa agattatgac tgtgattacg acaaaaaata tgatgactat gataaaaaat
     1201 attatgatca cgataaaaaa gactatgatt atgttgtaga gtataaaaag cataaaaaac
     1261 actactaaac gccattaaca tctcctcgtt tttactttcc cccggctatt gccgggtctt
     1321 ttttgtttgt gtctagagaa tttgagatat tacgtacctt taatacatta ttcatgattt
     1381 tttcataatg aggagaagga tatttgaact aaaaatatcc ttactgaagt taagcgttga
     1441 ttttcattct ttcatctgct aacatattat catattagcg aactaaagga ttcggaattt
     1501 tccttcataa gataagtcga cgttgtggaa ggagagacgg caaaatgaag atcaaaacca
     1561 tcagcggagc ggaacggctt tctctcaagc gttccatcga tgccggaacg gaagagcaaa
     1621 gaaagacggt ccggtccatt attgaagatg ttaaagcaaa cggtgatcag gcggttcgca
     1681 gctacacggc gaaatttgac tgcatcgaaa tagacagccc gctggtcaca aaagaagaat
     1741 ttgaggaggc atacacctca ctggattcac gtcttcttca ggtgattagg caggcaatcg
     1801 aaaatattag ggaatatcac gaaagacagc tgcaatcctc ctggttttat cacagaaagg
     1861 acggcacaat gctggggcag aaagtgacgg cgctggattc cgccggtgta tatgtgccgg
     1921 ggggaacggc cgcatatcct tcatctgttt tgatgaatgt gattccggcg ctcgtagcgg
     1981 gcgttgaaag aatcgttctc gtcacgcctc cgggaaaaga cggtctgctg tctcctggag
     2041 ttttggttgc tgcagccgag ctgggcataa aagacattta caaaatgggc ggcgctcagg
     2101 cgattgcggc gttagcatac ggaacagaaa ccattgagcc ggtggataaa atcacgggtc
     2161 cgggcaacat ttatgtcgcg cttgccaaac gagaagtgtt cggcgatgtc gatatcgata
     2221 tgattgccgg accgagtgaa atcgtcgtgc tggctgatga aacagcgatt ccgagcgaaa
     2281 ttgcagccga tctgctgtca caggcggaac acgataagct tagctcatgt gtgtttgtca
     2341 cagattccat ggcgctggcc gagacggttt cagccgaggt gaataagcag ctggaaacct
     2401 tgccgcgaag agaaatagcg gaagcttcag tgagagatta cggttgtata tatgtggcgg
     2461 aaacaatggt agaagcgatt gagactgtca atacactcgc gccagagcat ttagaaatca
     2521 taacacagtc tccggaagct ttgcttggca gtatcaaaca tgcgggtgca attttcttag
     2581 gcagatacag ccctgagcct gtcggtgatt attttgccgg accgaaccat gtgcttccga
     2641 caaacggaac agccagattt tcaagtccat taaacgtaac tgattttcaa aagaaatcga
     2701 gcattatttc ttacagtcaa tcagcctttg aagaacatgc cgaaagcatc gcggcttttg
     2761 ccagactgga agggttagaa gcccatgcca gatcaattga agcgagagaa cggaggattt
     2821 ctaaatgaga aaagcggaag catgctggca taaatcacag tggcaatctc ccccgtattc
     2881 gtttgaaatg tgccacatta acagcgccgg gtgatgtccg tatcgttctg ctaataagcg
     2941 gttgatgtgc cgtgtttttt ctcggtagac tttagatgtg aggcagtggt tgtgccttcc
     3001 gccgtgcagc tgtttgacgc gggaggcatt gacgcgcaaa acttccggat aggtttgcga
     3061 cagccaggcc ggacgggctc cgctcggcgt tgctaatatg acccggccgc ctatactgtg
     3121 aatccgctca aaaatatcat ccagccattc aaattgatat acgccctcct ccggctcaag
     3181 tgcgctccat gcaaaaatgc cgacagaaaa cgtattcgta tgagaaagct tggcgtaatc
     3241 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     3301 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     3361 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     3421 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     3481 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     3541 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     3601 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     3661 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     3721 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     3781 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     3841 atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     3901 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     3961 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     4021 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     4081 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     4141 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     4201 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     4261 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     4321 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     4381 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     4441 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     4501 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     4561 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     4621 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     4681 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     4741 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     4801 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     4861 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     4921 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     4981 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     5041 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     5101 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     5161 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     5221 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     5281 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     5341 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     5401 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
     5461 tcgtc
//
