pCotB-NL vector (V008282)

LOCUS       V008282                 8276 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008282
VERSION     V008282
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8276)
  AUTHORS   Iwanicki A, Hinc K, Stasilojc M, Piatek I, Grela A, Lega T,
            Obuchowski M.
  TITLE     Vector system for spore sufrace display
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8276)
  AUTHORS   Iwanicki A, Hinc K, Stasilojc M, Piatek I, Grela A, Lega T,
            Obuchowski M.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-OCT-2013) Laboratory of Molecular Bacteriology,
            Medical University of Gdansk, Debinki 1, Gdansk 80-211, Poland
REFERENCE   3  (bases 1 to 8276)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8276)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Vectro NTI Advanced v. 10
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (24-OCT-2013) Laboratory of Molecular Bacteriology, Medical
            University of Gdansk, Debinki 1, Gdansk 80-211, Poland"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8276
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        106..210
                     /label="AmpR promoter"
     CDS             211..1068
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      1242..1830
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(2016..2156)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     CDS             complement(2261..2449)
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     CDS             complement(3705..4724)
                     /codon_start=1
                     /gene="amyE"
                     /product="AmyE"
                     /label="amyE"
                     /note="C-terminal part of amyE"
                     /protein_id="AHG54598.1"
                     /translation="MSDDDIRLGWAVIASRSGSTPLFFSRPEGGGNGVRFPGKSQIGDR
                     GSALFEDQAITAVNRFHNVMAGQPEELSNPNGNNQIFMNQRGSHGVVLANAGSSSVSIN
                     TATKLPDGRYDNKAGAGSFQVNDGKLTGTINARSVAVLYPDDIAKAPHVFLENYKTGVT
                     HSFNDQLTITLRADANTTKAVYQINNGPDDRRLRMEINSQSEKEIQFGKTYTIMLKGTN
                     SDGVTRTEKYSFVKRDPASAKTIGYQNPNHWSQVNAYIYKHDGSRVIELTGSWPGKPMT
                     KNADGIYTLTLPADTDTTNAKVIFNNGSAQVPGQNQPGFDYVLNGLYNDSGLSGSLPH"
     gene            complement(3705..4724)
                     /gene="amyE"
                     /label="amyE"
     misc_feature    4825..4959
                     /gene="cotH"
                     /note="CotH; C-terminal part of cotH"
     gene            4825..4959
                     /gene="cotH"
                     /label="cotH"
     gene            5011..6022
                     /gene="cotB"
                     /label="cotB"
     regulatory      5011..5050
                     /gene="cotB"
                     /regulatory_class="promoter"
     regulatory      5074..5081
                     /gene="cotB"
                     /regulatory_class="ribosome_binding_site"
     CDS             5087..6022
                     /codon_start=1
                     /gene="cotB"
                     /product="CotB"
                     /label="cotB"
                     /note="spore coat protein"
                     /protein_id="AHG54601.1"
                     /translation="MSKRRMKTRYVKLPWARGRASRSGTSGGGSGTSNNEISYYNFLHS
                     MKDKIVTVYRGGPESKKGKLTAVKSDYIALQAEKKIIYYQLEHVKSITEDTNNSTTTIE
                     TEEMLDADDFHSLIGHLINQSVQFNQGGPESKKGRLVWLGDDYAALNTNEDGVVYFNIH
                     HIKSISKHEPDLKIEEQTPVGVLEADDLSEVFKSLTHKWVSINRGGPEAIEGILVDNAD
                     GHYTIVKNQEVLRIYPFHIKSISLGPKGSYKKEDQKNEQNQEDNNDKDSNSFISSKSYS
                     SSKSSKRSLKSSDDQSSGSWNFEDPLEIRK"
     misc_feature    5114..5160
                     /gene="cotB"
                     /label="multiple cloning site"
                     /note="multiple cloning site"
     regulatory      6084..6112
                     /gene="thrC"
                     /regulatory_class="promoter"
     CDS             6210..7265
                     /gene="thrC"
                     /label="Threonine synthase"
                     /note="Threonine synthase from Bacillus subtilis (strain
                     168). Accession#: P04990"
     CDS             complement(7629..8162)
                     /codon_start=1
                     /gene="amyE"
                     /product="AmyE"
                     /label="amyE"
                     /note="N terminal part of amyE"
                     /protein_id="AHG54599.1"
                     /translation="MFAKRFKTSLLPLFAGFLLLFHLVLAGPAAASAETANKSNELTAP
                     SIKSGTILHAWNWSFNTLKHNMKDIHDAGYTAIQTSPINQVKEGNQGDKSMSNWYWLYQ
                     PTSYQIGNRYLGTEQEFKEMCAAAEEYGIKVIVDAVINHTTSDYAAISNEVKSIPNWTH
                     GNTQIKNWSDRNST"
     gene            complement(7629..8162)
                     /gene="amyE"
                     /label="amyE"