LOCUS       V008282                 8276 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008282
VERSION     V008282
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8276)
  AUTHORS   Iwanicki A, Hinc K, Stasilojc M, Piatek I, Grela A, Lega T,
            Obuchowski M.
  TITLE     Vector system for spore sufrace display
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8276)
  AUTHORS   Iwanicki A, Hinc K, Stasilojc M, Piatek I, Grela A, Lega T,
            Obuchowski M.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-OCT-2013) Laboratory of Molecular Bacteriology,
            Medical University of Gdansk, Debinki 1, Gdansk 80-211, Poland
REFERENCE   3  (bases 1 to 8276)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8276)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Vectro NTI Advanced v. 10
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (24-OCT-2013) Laboratory of Molecular Bacteriology, Medical
            University of Gdansk, Debinki 1, Gdansk 80-211, Poland"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8276
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        106..210
                     /label="AmpR promoter"
     CDS             211..1068
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      1242..1830
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(2016..2156)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     CDS             complement(2261..2449)
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     CDS             complement(3705..4724)
                     /codon_start=1
                     /gene="amyE"
                     /product="AmyE"
                     /label="amyE"
                     /note="C-terminal part of amyE"
                     /protein_id="AHG54598.1"
                     /translation="MSDDDIRLGWAVIASRSGSTPLFFSRPEGGGNGVRFPGKSQIGDR
                     GSALFEDQAITAVNRFHNVMAGQPEELSNPNGNNQIFMNQRGSHGVVLANAGSSSVSIN
                     TATKLPDGRYDNKAGAGSFQVNDGKLTGTINARSVAVLYPDDIAKAPHVFLENYKTGVT
                     HSFNDQLTITLRADANTTKAVYQINNGPDDRRLRMEINSQSEKEIQFGKTYTIMLKGTN
                     SDGVTRTEKYSFVKRDPASAKTIGYQNPNHWSQVNAYIYKHDGSRVIELTGSWPGKPMT
                     KNADGIYTLTLPADTDTTNAKVIFNNGSAQVPGQNQPGFDYVLNGLYNDSGLSGSLPH"
     gene            complement(3705..4724)
                     /gene="amyE"
                     /label="amyE"
     misc_feature    4825..4959
                     /gene="cotH"
                     /note="CotH; C-terminal part of cotH"
     gene            4825..4959
                     /gene="cotH"
                     /label="cotH"
     gene            5011..6022
                     /gene="cotB"
                     /label="cotB"
     regulatory      5011..5050
                     /gene="cotB"
                     /regulatory_class="promoter"
     regulatory      5074..5081
                     /gene="cotB"
                     /regulatory_class="ribosome_binding_site"
     CDS             5087..6022
                     /codon_start=1
                     /gene="cotB"
                     /product="CotB"
                     /label="cotB"
                     /note="spore coat protein"
                     /protein_id="AHG54601.1"
                     /translation="MSKRRMKTRYVKLPWARGRASRSGTSGGGSGTSNNEISYYNFLHS
                     MKDKIVTVYRGGPESKKGKLTAVKSDYIALQAEKKIIYYQLEHVKSITEDTNNSTTTIE
                     TEEMLDADDFHSLIGHLINQSVQFNQGGPESKKGRLVWLGDDYAALNTNEDGVVYFNIH
                     HIKSISKHEPDLKIEEQTPVGVLEADDLSEVFKSLTHKWVSINRGGPEAIEGILVDNAD
                     GHYTIVKNQEVLRIYPFHIKSISLGPKGSYKKEDQKNEQNQEDNNDKDSNSFISSKSYS
                     SSKSSKRSLKSSDDQSSGSWNFEDPLEIRK"
     misc_feature    5114..5160
                     /gene="cotB"
                     /label="multiple cloning site"
                     /note="multiple cloning site"
     regulatory      6084..6112
                     /gene="thrC"
                     /regulatory_class="promoter"
     CDS             6210..7265
                     /gene="thrC"
                     /label="Threonine synthase"
                     /note="Threonine synthase from Bacillus subtilis (strain
                     168). Accession#: P04990"
     CDS             complement(7629..8162)
                     /codon_start=1
                     /gene="amyE"
                     /product="AmyE"
                     /label="amyE"
                     /note="N terminal part of amyE"
                     /protein_id="AHG54599.1"
                     /translation="MFAKRFKTSLLPLFAGFLLLFHLVLAGPAAASAETANKSNELTAP
                     SIKSGTILHAWNWSFNTLKHNMKDIHDAGYTAIQTSPINQVKEGNQGDKSMSNWYWLYQ
                     PTSYQIGNRYLGTEQEFKEMCAAAEEYGIKVIVDAVINHTTSDYAAISNEVKSIPNWTH
                     GNTQIKNWSDRNST"
     gene            complement(7629..8162)
                     /gene="amyE"
                     /label="amyE"
ORIGIN
        1 aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
       61 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
      121 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
      181 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
      241 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
      301 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
      361 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
      421 aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt
      481 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
      541 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
      601 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
      661 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
      721 ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa
      781 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
      841 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
      901 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
      961 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     1021 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     1081 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     1141 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     1201 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     1261 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     1321 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     1381 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     1441 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     1501 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     1561 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     1621 ctacagcgtg agcattgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     1681 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     1741 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     1801 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     1861 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     1921 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     1981 cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg
     2041 catctgtgcg gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc
     2101 gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc
     2161 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     2221 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     2281 cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga
     2341 tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc
     2401 ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact tgatgcctcc
     2461 gtgtaagggg gaatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct
     2521 cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca
     2581 actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt
     2641 cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg
     2701 gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc
     2761 gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt
     2821 tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg
     2881 ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga
     2941 gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt
     3001 ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa
     3061 tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg
     3121 cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg
     3181 ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa
     3241 gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc
     3301 tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc
     3361 ataatgggga aggccatcca gcctcgcgtc gcgactaaga aaatgccgtc aaatccgctc
     3421 gccatgactt cactaacgat gcctttgaaa atcttcaagt tcttttctac taattcaagg
     3481 cgtgtctcac caggtttttg gtttgctccg gcgcaaatgc agacaatatc agcatccttg
     3541 cagggtatgt ttctctttga tgtctttttg tttgtgaagt atttcacatt tatattgtgc
     3601 aacacttcac aaacttttgc aagagaaaag ttttgtctga tttatgaaca aaaaagaaac
     3661 catcattgat ggtttctttc ggtaagtccc gtctagcctt gccctcaatg gggaagagaa
     3721 ccgcttaagc ccgagtcatt atataaacca tttagcacgt aatcaaagcc aggctgattc
     3781 tgaccgggca cttgggcgct gccattatta aaaatcactt ttgcgttggt tgtatccgtg
     3841 tccgcaggca gcgtcagcgt gtaaattccg tctgcatttt tagtcattgg ttttccaggc
     3901 caagatccgg tcaattcaat tactcggctc ccatcatgtt tatagatata agcatttacc
     3961 tggctccaat gattcggatt ttgatagccg atggttttgg ccgacgctgg atctctttta
     4021 acaaaactgt atttctcggt cctcgttaca ccatcactgt tcgttccttt taacatgatg
     4081 gtgtatgttt tgccaaattg gatctccttt tccgattgtg aattgatctc catccttaaa
     4141 cgcctgtcgt ctggtccatt attgatttga taaacggctt ttgttgtatt cgcatctgca
     4201 cgcaaggtaa tcgtcagttg atcattgaaa gaatgtgtta cacctgtttt gtaattctca
     4261 aggaaaacat gaggcgcttt tgcaatatca tcaggataaa gcacagctac agacctggca
     4321 ttgatcgtgc ctgtcagttt accatcgttc acttgaaatg aacccgctcc agctttattg
     4381 tcatacctgc catcaggcaa ttttgttgcc gtattgatag agacagagga tgaacctgca
     4441 tttgccagca caacgccatg tgagccgcgc tgattcataa atatctggtt gtttccattc
     4501 gggttcgaga gttcctcagg ctgtccagcc atcacattgt gaaatctatt gaccgcagtg
     4561 atagcctgat cttcaaataa agcactcccg cgatcgccta tttggctttt ccccgggaac
     4621 ctcacaccat ttccgcctcc ctcaggtctg gaaaagaaaa gaggcgtact gcctgaacga
     4681 gaagctatca ccgcccagcc taaacggata tcatcatcgc tcatccatgt cgacgctctc
     4741 ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg
     4801 ccgccgcaag gaatggtgca tgcacggatt aggccgtttg tcctcatgga cccgtataaa
     4861 aagaatgata ttgagcgttt tgaccgtgag ccggatgtga tctgcgagta tattaaaaac
     4921 cgttcacaat acctcaaaga tcatttaagt attttatgaa tgcgtgaaaa tgggtattcg
     4981 cggaaaaagc gacaattagg ctattgaatt agttcaacaa ataaatgtga cacgtatata
     5041 tgcagtatgt ttatcatcta tgtataagtg actaggagga atttgaatga gcaagaggag
     5101 aatgaaaacg cgttacgtaa agcttccatg ggcccgcggc cgcgctagca gatctggtac
     5161 ctcaggagga ggatcaggta cctcaaataa tgaaatatcg tattataact ttttgcactc
     5221 aatgaaagat aaaattgtta ctgtatatcg tggaggtccg gaatctaaaa aaggaaaatt
     5281 aacagctgta aaatcagatt atatagcttt acaagctgaa aaaaaaataa tttattatca
     5341 gttggagcat gtgaaaagta ttactgagga taccaataat agcaccacaa caattgagac
     5401 tgaggaaatg ctcgatgctg atgattttca tagcttaatc ggacatttaa taaaccaatc
     5461 agttcaattt aaccaagggg gtccggaatc taaaaaagga agattggtct ggctgggaga
     5521 tgattacgct gcgttaaaca caaatgagga tggggtagtg tattttaata tccatcacat
     5581 caaaagtata agtaaacacg agcctgattt gaaaatagaa gagcagacgc cagttggagt
     5641 tttggaagct gatgatttaa gcgaggtttt taagagtctg actcataaat gggtttcaat
     5701 taatcgtgga ggtccggaag ccattgaggg tatccttgta gataatgccg acggccatta
     5761 tactatagtg aaaaatcaag aggtgcttcg catctatcct tttcacataa aaagcatcag
     5821 cttaggtcca aaagggtcgt acaaaaaaga ggatcaaaaa aatgaacaaa accaggaaga
     5881 caataatgat aaggacagca attcgttcat ttcttcaaaa tcatatagct catcaaaatc
     5941 atctaaacga tcactaaaat cttcagatga tcaatcatcc ggatcctgga atttcgagga
     6001 tcctctagaa attagaaaat aaagaatctt gtcatatcaa tgcgttaagc gtttacattc
     6061 gcaaaattca gcctaatgat gtcttgtcaa aacaggagcg ggcttatata atgtttgtaa
     6121 cttatatgtc tgtgttgagt taacaattgc ctgcaggtcg ttcaagaagt caaaagcaca
     6181 tatcgtgtag aagggaacgg ttggagctaa tgtggaaagg acttatccat caatataaag
     6241 aatttttacc tgtaacagat caaacaccgg cgctaacttt acatgaagga aacacacctc
     6301 ttattcacct gccgaagctg tctgagcagc tcggaattga gcttcatgtc aaaacggaag
     6361 gcgtcaatcc tacgggatca tttaaagatc gcggaatggt tatggctgtg gcaaaggcaa
     6421 aagaagaagg caatgacacg attatgtgcg cgtcaacagg taacacttcc gctgctgcgg
     6481 cagcatatgc agcccgtgct aacatgaaat gcattgtcat catcccgaac ggaaaaattg
     6541 catttggaaa actcgctcaa gctgtcatgt acggagccga gattatcgca attgacggaa
     6601 actttgacga tgcgcttaaa attgtccgtt ccatctgtga gaaatcaccg attgcccttg
     6661 tcaactcagt caacccttac cgcattgaag gccaaaaaac tgctgccttc gaagtgtgcg
     6721 aacagctggg ggaagcgcct gatgttttag cgatcccggt cggaaatgct ggaaacatca
     6781 ctgcgtactg gaagggcttc aaggaatatc atgagaaaaa cggcacaggc cttccgaaaa
     6841 tgcgcggctt tgaagctgag ggtgcggcgg caatcgtgcg caatgaagtg attgaaaatc
     6901 cggaaacaat agcgacagcc attcgtatcg gaaacccggc aagctgggac aaagctgtaa
     6961 aggcagccga ggaatccaat gggaaaattg acgaagtcac tgatgatgaa atccttcacg
     7021 catatcagct gatcgcccgt gtagaaggcg tgtttgcaga accaggttct tgcgcgtcta
     7081 tcgcaggagt gctgaaacag gtgaaatccg gagaaattcc gaaaggcagc aaggtcgtag
     7141 ctgtgttaac aggaaacgga ctgaaagatc cgaacacagc ggtcgacatt tcagaaatca
     7201 agcctgtcac attgccgact gatgaagaca gcatccttga atatgtaaaa ggagcggccc
     7261 gtgtatgaac gaagccgaca tgctgttctc gtacgtcttc ccgagcgaaa acggtctgcg
     7321 ctgcgggacg cgcgaattga attatggccc acaccagtgg cgcggcgact tccagttcaa
     7381 catcagccgc tacagtcaac agcaactgat ggaaaccagc catcgccatc tgctgcacgc
     7441 ggaagaaggc acatggctga atatcgacgg tttccatatg gggattggtg gcgacgactc
     7501 ctggagcccg tcagtatcgg cggaaatcca gctgagcgcc ggtcgctacc attaccagtt
     7561 ggtctggtgt caaaaataat aataaccggg caggccatgt ctgcccgtat ttcgcgtaag
     7621 gaaatccatt atgtactatt tcgatcagac cagtttttaa tttgtgtgtt tccatgtgtc
     7681 cagtttggaa tactcttaac ctcattggaa atcgcggcat aatcactggt ggtatgattg
     7741 atgaccgcgt caacaatgac ctttatgcca tattcttcag cggctgcaca catttcttta
     7801 aattcttgtt cagtacctaa gtaacggttg ccaatttgat acgatgtcgg ctgatacagc
     7861 cagtaccagt tcgacatgct tttatctcct tgattccctt cctttacttg gttaatcgga
     7921 gatgtctgaa tggctgtata tcctgcatca tgaatatcct tcatattgtg ttttaacgta
     7981 ttgaacgacc aattccatgc atgaagaatg gttccgcttt tgatcgacgg tgctgtaagc
     8041 tcattcgatt tgttcgccgt ttcagcactc gcagccgccg gtcctgccag aaccaaatga
     8101 aacagcaata aaaatccagc gaataacggc agtaaagagg ttttgaatcg ttttgcaaac
     8161 attcttgaca ctccttattt gattttttga agacttactt cggagtcaaa aatccctctt
     8221 acttcattct tccgcttcct cctttcaaac cgatgtgaag actggagaat tttgtt
//
