pCon2(3) vector (V008293)

LOCUS       V008293                 7692 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008293
VERSION     V008293
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7692)
  AUTHORS   Raja Abd Rahman RNZ., Wong CF, Salleh AB, Basri M.
  TITLE     Functions of Plac and PT7(A1/04/03) in overexpression of elastase
            strain K by E. coli and Pseudomonas sp. harbouring pCon2(3)##
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7692)
  AUTHORS   Raja Abd Rahman RNZ., Wong CF, Salleh AB, Basri M.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-JAN-2011) Department of Microbiology, Universiti Putra
            Malaysia, Faculty of Biotechnology and Biomolecular Sciences, UPM
            Serdang, Selangor 43400, Malaysia
REFERENCE   3  (bases 1 to 7692)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7692)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (31-JAN-2011) Department of Microbiology, Universiti Putra Malaysia,
            Faculty of Biotechnology and Biomolecular Sciences, UPM Serdang,
            Selangor 43400, Malaysia"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7692
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(317..1810)
                     /gene="lasB"
                     /label="Elastase"
                     /note="Elastase from Pseudomonas aeruginosa (strain ATCC
                     15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C
                     / PRS 101 / PAO1). Accession#: P14756"
     rep_origin      complement(2649..3000)
                     /direction=LEFT
                     /label="pRO1600 oriV"
                     /note="broad-host-range origin of replication from
                     Pseudomonas aeruginosa plasmid pRO1600; requires the
                     pRO1600 Rep protein for replication (West et al., 1994)"
     CDS             3014..3844
                     /label="pRO1600 Rep"
                     /note="replication protein for the broad-host-range plasmid
                     pRO1600 from Pseudomonas aeruginosa"
     primer_bind     4008..4024
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             complement(4075..5568)
                     /gene="lasB"
                     /label="Elastase"
                     /note="Elastase from Pseudomonas aeruginosa (strain ATCC
                     15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C
                     / PRS 101 / PAO1). Accession#: P14756"
     primer_bind     complement(5588..5604)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(5612..5628)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5636..5666)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5681..5702)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5990..6578)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(6752..7609)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(join(7610..7692,1..22))
                     /label="AmpR promoter"