LOCUS       V008293                 7692 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008293
VERSION     V008293
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7692)
  AUTHORS   Raja Abd Rahman RNZ., Wong CF, Salleh AB, Basri M.
  TITLE     Functions of Plac and PT7(A1/04/03) in overexpression of elastase
            strain K by E. coli and Pseudomonas sp. harbouring pCon2(3)##
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7692)
  AUTHORS   Raja Abd Rahman RNZ., Wong CF, Salleh AB, Basri M.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-JAN-2011) Department of Microbiology, Universiti Putra
            Malaysia, Faculty of Biotechnology and Biomolecular Sciences, UPM
            Serdang, Selangor 43400, Malaysia
REFERENCE   3  (bases 1 to 7692)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7692)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (31-JAN-2011) Department of Microbiology, Universiti Putra Malaysia,
            Faculty of Biotechnology and Biomolecular Sciences, UPM Serdang,
            Selangor 43400, Malaysia"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7692
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(317..1810)
                     /gene="lasB"
                     /label="Elastase"
                     /note="Elastase from Pseudomonas aeruginosa (strain ATCC
                     15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C
                     / PRS 101 / PAO1). Accession#: P14756"
     rep_origin      complement(2649..3000)
                     /direction=LEFT
                     /label="pRO1600 oriV"
                     /note="broad-host-range origin of replication from
                     Pseudomonas aeruginosa plasmid pRO1600; requires the
                     pRO1600 Rep protein for replication (West et al., 1994)"
     CDS             3014..3844
                     /label="pRO1600 Rep"
                     /note="replication protein for the broad-host-range plasmid
                     pRO1600 from Pseudomonas aeruginosa"
     primer_bind     4008..4024
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             complement(4075..5568)
                     /gene="lasB"
                     /label="Elastase"
                     /note="Elastase from Pseudomonas aeruginosa (strain ATCC
                     15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C
                     / PRS 101 / PAO1). Accession#: P14756"
     primer_bind     complement(5588..5604)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(5612..5628)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5636..5666)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5681..5702)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5990..6578)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(6752..7609)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(join(7610..7692,1..22))
                     /label="AmpR promoter"
ORIGIN
        1 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
       61 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg
      121 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
      181 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
      241 gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc
      301 atatgaagct tttttacaac gcgctcgggc aggtcacgcc gacggtgctg aacgcccggg
      361 tgacgtcagc cgccgagtag ttgcggttct gcgccgagcg aatcaccccg caggcgccgc
      421 tgttgtagtt gctggtggcg gtccagtagt agcggttggc gtcgacgaac acctcgaagg
      481 ccttgcgggt atcccagccc ggcgaattgg ccaacaggta gaacgcacgg ttgtacacgc
      541 cgctggagtg gtgcacgtcg atgccgttgt agtactgcga cgcgttgtcg atggatcgcc
      601 cgtcgcggct gggctggtcc atgtagcgca gcgcaccgct gcccttcttg atgtcgtagc
      661 cgatcaggaa gtcgttcttg ccgcgcatgt agaattcggc tgcctcgccg gccatgtcgg
      721 agaacgcttc gttcattccg cctgattgcc cgcggtagat cagcccggag ttctgctcgg
      781 taaagccgtg gctgacctcg tgggccgcca cgtccagcga caccagcgga tagaacatgg
      841 tggcgccgtc gccgaagagc atcgccgtgc cgccccagta ggcgttctcc acgctgcgcc
      901 cgtagtgcac cttcatgtac agcttgtggg tcagcgggct ggtgccgaac cagtcccggt
      961 acagtttgaa caccacgccg ccgaagaaat gcgcgtcgtt cagcggcgaa taggcgccgt
     1021 tgacctgctt gtaggtgttg gtcgggcagg cgaagcggaa cggcgtggtc ttgctgtcgt
     1081 cggtgctgcc gttcatgtcg acggtgatga cgttgccgtc gtccatctcg cagcggtcgt
     1141 tgacgatcag cggaccgtag tcgctaccgt aggtgtactt gccgatcttc tggttgccgc
     1201 cggggccgcc cgcctcggcg tgggccaggc cttcccactg atcgagcact tcgccggtct
     1261 tggcgtcgat gacgaaatgc ggccgcgaca gtccctcgcc gggaatcagg taggagacgt
     1321 tgtagaccag ttgggcgatg ttgctctcgc ccaggcggat caccagttcc actttgtcat
     1381 tctcggtctt gcggccctgg gccttcaggc tcttggcctg ggccagcacc tgctcggcgg
     1441 ataccgccgc ggtggtgctg cccggcaggt cggcagcgat gttggcgacg aaatggccgc
     1501 tgcgctgcgc cgccacgctc ttgccgggac ccttgacttc ggtgatggct tcgccgacca
     1561 cccgtacgcc gttgtggaat tgctcgtagc gggtgacctg cttgccgttg ggcagggtcg
     1621 tgctgcggat cgctttcagt tcatcggcac cgccagcgcc gaccgcggct tgcaaggtga
     1681 ccgggccggg cgcgccctgg gcagccttgc tggggagttt ggacacgtcg atcaggtcgg
     1741 cggcaagagc ggccggcgaa acacccatga tcgcaacgaa caacaggtca agcgtagaaa
     1801 ccttcttcat tctgcaggtc gactctagag gatccccggg taccgagctc gaattctgtg
     1861 tgaaattgtt atccgctcac aattgaatct aagtatcatt gttatccgct cacaagtcaa
     1921 cactcttttt gataaatttt catatgcggt gtgaaatacc gcacagatgc gtaaggagaa
     1981 aataccgcat caggcggcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     2041 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     2101 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     2161 gcgtggatct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     2221 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     2281 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     2341 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     2401 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccaa
     2461 ttacacgcca ctggctgtgc ttgctggggt gacggtggca acggtggcgg ccttgctggg
     2521 ctatcgcgtt ggaaagaaac gagggaaagg ggactgataa accggtctta gcccctcccc
     2581 ttggtgtcca accgctctgt aggcctctca ggcgccgctg gtgccgctgg ttggacgcca
     2641 agggtgaatc cgcctcgata ccctgattac tcgcttcctg cgccctctca ggcggcgata
     2701 ggggactggt aaaacgggga ttgcccagac gcctcccccg ccccttcagg ggcacaaatg
     2761 cggccccaac ggggccacgt agtggtgcgt tttttgcgtt tccacccttt tcttcctttt
     2821 cccttttaaa ccttttagga cgtctacagg ccacgtaatc cgtggcctgt agagtttaaa
     2881 aagggacgga tttgttgcca ttaagggacg gatttgttgt taagaaggga cggatttgtt
     2941 gttgtaaagg gacggatttg ttgtattgtg ggacgcagat acagtgtccc cttatacaca
     3001 aggaatgtcg aacgtggcct cacccccaat ggtttacaaa agcaatgccc tggtcgaggc
     3061 cgcgtatcgc ctcagtgttc aggaacagcg gatcgttctg gcctgtatta gccaggtgaa
     3121 gaggagcgag cctgtcaccg atgaagtgat gtattcagtg acggcggagg acatagcgac
     3181 gatggcgggt gtccctatcg aatcttccta caaccagctc aaagaagcgg ccctgcgcct
     3241 gaaacggcgg gaagtccggt taacccaaga gcccaatggc aaggggaaaa gaccgagtgt
     3301 gatgattacc ggctgggtgc aaacaatcat ctaccgggag ggtgagggcc gtgtagaact
     3361 caggttcacc aaagacatgc tgccgtacct gacggaactc accaaacagt tcaccaaata
     3421 cgccttggct gacgtggcca agatggacag cacccacgcg atcaggcttt acgagctgct
     3481 catgcaatgg gacagcatcg gccagcgcga aatagaaatt gaccagctgc gaaagtggtt
     3541 tcaactggaa ggccggtatc cctcgatcaa ggacttcaag ttgcgagtgc ttgatccagc
     3601 cgtgacgcag atcaacgagc acagcccgct acaggtggag tgggcgcagc gaaagaccgg
     3661 gcgcaaggtc acacatctgt tgttcagttt tggaccgaag aagcccgcca aggcggtggg
     3721 taaggcccca gcgaagcgca aggccgggaa gatttcagat gctgagatcg cgaaacaggc
     3781 tcgccctggt gagacatggg aagcggcccg cgctcgacta acccagatgc cgctggatct
     3841 ggcctagagg ccgtggccac cacggcccgg cctgcctttc aggctgcgca actgttggga
     3901 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
     3961 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
     4021 cagtgaattc gagctcggta cccggggatc ctctagagtc gacctgcagt tttacaacgc
     4081 gctcgggcag gtcacgccga cggtgctgaa cgcccgggtg acgtcagccg ccgagtagtt
     4141 gcggttctgc gccgagcgaa tcaccccgca ggcgccgctg ttgtagttgc tggtggcggt
     4201 ccagtagtag cggttggcgt cgacgaacac ctcgaaggcc ttgcgggtat cccagcccgg
     4261 cgaattggcc aacaggtaga acgcacggtt gtacacgccg ctggagtggt gcacgtcgat
     4321 gccgttgtag tactgcgacg cgttgtcgat ggatcgcccg tcgcggctgg gctggtccat
     4381 gtagcgcagc gcaccgctgc ccttcttgat gtcgtagccg atcaggaagt cgttcttgcc
     4441 gcgcatgtag aactcggctg cctcgccggc catgtcggag aacgcttcgt tcattccgcc
     4501 tgattgcccg cggtagatca gcccggagtt ctgctcggta aagccgtggc tgacctcgtg
     4561 ggccgccacg tccagcgaca ccagcggata gaacatggtg gcgccgtcgc cgaagagcat
     4621 cgccgtgccg tcccagtagg cgttctccac gctgcgcccg tagtgcacct tcatgtacag
     4681 cttgtgggtc agcgggctgg tgccgaacca gtcccggtac agtttgaaca ccacgccgcc
     4741 gaagaaatgc gcgtcgttca gcggcgaata ggcgccgttg acctgcttgt aggtgttggt
     4801 cgggcaggcg aagcggaacg gcgtggtctt gctgtcgtcg gtgctgccgt tcatgtcgac
     4861 ggtgatgacg ttgccgtcgt ccatctcgca gcggtcgttg acgatcagcg gaccgtagtc
     4921 gctaccgtag gtgtacttgc cgatcttctg gttgccgccg gggccgcccg cctcggcgtg
     4981 ggccaggcct tcccactgat cgagcacttc gccggtcttg gcgtcgatga cgaaatgcgg
     5041 ccgcgacagt ccctcgccgg gaatcaggta ggagacgttg tagaccagtt gggcgatgtt
     5101 gttctcgccc aggcggatca ccagttccac tttgtcattc tcggtcttgc ggccctgggc
     5161 cttcaggctc ttggcctggg ccagcacctg ctcggcggat accgccgcgg tggtgctgcc
     5221 cggcaggtcg gcagcgatgt tggcgacgaa atggccgctg cgctgcgccg ccacgctctt
     5281 gccgggaccc ttgacttcgg tgatggcttc gccgaccacc cgtacgccgt tgtggaattg
     5341 ctcgtagcgg gtgacctgct tgccgttggg cagggtcgtg ctgcggatcg ctttcagttc
     5401 atcggcaccg ccagcgccga ccgcggcttg caaggtgacc gggccgggcg cgccctgggc
     5461 agccttgctg gggagtttgg acacgtcgat caggtcggcg gcaaaagcgg ccggcgaaac
     5521 acccatgatc gcaacgaaca acaggtcaag cgtagaaacc ttcttcatta agcttggcgt
     5581 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     5641 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     5701 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     5761 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     5821 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     5881 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     5941 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     6001 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     6061 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     6121 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     6181 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     6241 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     6301 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     6361 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     6421 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     6481 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     6541 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     6601 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     6661 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     6721 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     6781 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     6841 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     6901 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     6961 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     7021 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     7081 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     7141 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     7201 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     7261 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     7321 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     7381 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     7441 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     7501 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     7561 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     7621 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     7681 gtatttagaa aa
//
