Basic Vector Information
- Vector Name:
- PCMV6-XL3
- Antibiotic Resistance:
- Ampicillin
- Length:
- 4713 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Li X.
- Promoter:
- CMV
PCMV6-XL3 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
PCMV6-XL3 vector Sequence
LOCUS 40924_12320 4713 bp DNA circular SYN 17-DEC-2018 DEFINITION Cloning vector PCMV6-XL3, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4713) AUTHORS Li X. TITLE Direct Submission JOURNAL Submitted (20-MAY-1998) Origene Technologies Inc., 13 Taft Ct., Rockville, MD 20850, USA REFERENCE 2 (bases 1 to 4713) TITLE Direct Submission REFERENCE 3 (bases 1 to 4713) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Submitted (20-MAY-1998) Origene Technologies Inc., 13 Taft Ct., Rockville, MD 20850, USA" COMMENT SGRef: number: 2; type: "Journal Article" FEATURES Location/Qualifiers source 1..4713 /mol_type="other DNA" /organism="synthetic DNA construct" primer_bind 166..182 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" enhancer 343..722 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 723..926 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" promoter 952..970 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" misc_feature 972..1037 /label=multiple cloning site /note="multiple cloning site" promoter complement(1040..1058) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" polyA_signal 1068..1690 /label=hGH poly(A) signal /note="human growth hormone polyadenylation signal" promoter 1719..2048 /label=SV40 promoter /note="SV40 enhancer and early promoter" promoter complement(2087..2105) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(2119..2135) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind complement(2143..2159) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(2167..2197) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(2212..2233) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(2521..3109) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(3283..4140) /codon_start=1 /label=AmpR /note="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(4141..4245) /label=AmpR promoter rep_origin 4272..4700 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis"
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