PCMV6-XL3 vector (V008375)

Basic Vector Information

      • Vector Name:
      • PCMV6-XL3
      • Antibiotic Resistance:
      • Ampicillin
      • Length:
      • 4713 bp
      • Type:
      • Cloning vector
      • Source/Author:
      • Li X.

PCMV6-XL3 vector Vector Map

PCMV6-XL34713 bp600120018002400300036004200M13 fwdCMVT7 promotermultiple cloning siteT3 promoterhGH poly(A) signalSV40 promoterT7 promoterM13 revlac operatorlac promoterCAP binding siteColE1ampicillin resistance proteinAmpR promoterf1 ori

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

PCMV6-XL3 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       Exported                4713 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector PCMV6-XL3, complete sequence.
ACCESSION   AF067197
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4713)
  AUTHORS   Li X.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-1998) Origene Technologies Inc., 13 Taft Ct., 
            Rockville, MD 20850, USA
REFERENCE   2  (bases 1 to 4713)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4713
                     /organism="Cloning vector PCMV6-XL3"
                     /host="Escherichia coli"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:77617"
     primer_bind     166..182
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      201..926
                     /regulatory_class="promoter"
                     /note="CMV"
     enhancer        343..722
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        723..926
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        952..970
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      953..971
                     /regulatory_class="promoter"
                     /note="T7 promoter"
     misc_feature    972..1037
                     /label=multiple cloning site
                     /note="multiple cloning site"
     regulatory      1038..1058
                     /regulatory_class="promoter"
                     /note="T3 promoter"
     promoter        complement(1040..1058)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     polyA_signal    1068..1690
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     regulatory      1069..1690
                     /regulatory_class="polyA_signal_sequence"
                     /note="human growth hormone"
     promoter        1719..2048
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1747..2053
                     /label=SV40 origin
                     /note="SV40 origin"
     rep_origin      1899..2034
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(2087..2105)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2119..2135)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2143..2159
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2167..2197)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2212..2233
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      2463..3135
                     /label=ColE1
                     /note="ColE1"
     rep_origin      complement(2521..3109)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3280..4140)
                     /codon_start=1
                     /product="ampicillin resistance protein"
                     /label=ampicillin resistance protein
                     /protein_id="AAC24838.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4141..4245)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4272..4700
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     rep_origin      4273..4685
                     /label=f1
                     /note="f1"

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