LOCUS       40924_12320        4713 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector PCMV6-XL3, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4713)
  AUTHORS   Li X.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-1998) Origene Technologies Inc., 13 Taft Ct., 
            Rockville, MD 20850, USA
REFERENCE   2  (bases 1 to 4713)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 4713)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (20-MAY-1998) Origene Technologies Inc., 13 Taft Ct., Rockville, MD 
            20850, USA"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4713
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     166..182
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        343..722
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        723..926
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        952..970
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    972..1037
                     /label=multiple cloning site
                     /note="multiple cloning site"
     promoter        complement(1040..1058)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     polyA_signal    1068..1690
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     promoter        1719..2048
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     promoter        complement(2087..2105)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2119..2135)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2143..2159)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2167..2197)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2212..2233)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2521..3109)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3283..4140)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4141..4245)
                     /label=AmpR promoter
     rep_origin      4272..4700
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccgcga attcagatct ggtaccgata tcaagcttct agactgcagt
     1021 cgacggccaa gtcggcctcc ctttagtgag ggttaatttg tgatcccggg tggcatccct
     1081 gtgacccctc cccagtgcct ctcctggccc tggaagttgc cactccagtg cccaccagcc
     1141 ttgtcctaat aaaattaagt tgcatcattt tgtctgacta ggtgtccttc tataatatta
     1201 tggggtggag gggggtggta tggagcaagg ggcaagttgg gaagacaacc tgtagggcct
     1261 gcggggtcta ttgggaacca agctggagtg cagtggcaca atcttggctc actgcaatct
     1321 ccgcctcctg ggttcaagcg attctcctgc ctcagcctcc cgagttgttg ggattccagg
     1381 catgcatgac caggctcagc taatttttgt ttttttggta gagacggggt ttcaccatat
     1441 tggccaggct ggtctccaac tcctaatctc aggtgatcta cccaccttgg cctcccaaat
     1501 tgctgggatt acaggcgtga accactgctc ccttccctgt ccttctgatt ttaaaataac
     1561 tataccagca ggaggacgtc cagacacagc ataggctacc tggccatgcc caaccggtgg
     1621 gacatttgag ttgcttgctt ggcactgtcc tctcatgcgt tgggtccact cagtagatgc
     1681 ctgttgaatt gggtacgcgg ccagcttggc tgtggaatgt gtgtcagtta gggtgtggaa
     1741 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     1801 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     1861 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1921 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1981 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     2041 tttgcaaaaa gctcctcgag gaactgaaaa accagaaagt taattcccta tagtgagtcg
     2101 tattaaattc gtaatcatgt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     2161 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     2221 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     2281 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     2341 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     2401 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     2461 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     2521 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     2581 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     2641 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     2701 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     2761 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     2821 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     2881 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     2941 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     3001 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     3061 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     3121 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     3181 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     3241 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     3301 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     3361 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     3421 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     3481 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     3541 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     3601 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     3661 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     3721 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     3781 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     3841 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     3901 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     3961 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     4021 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     4081 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     4141 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     4201 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     4261 agtgccacct gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     4321 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     4381 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     4441 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     4501 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     4561 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     4621 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     4681 acaaaaattt aacgcgaatt ttaacaaaat att
//
