pCMIRT15 vector (V008416)

LOCUS       40924_11456       10759 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Binary vector pCMIRT15, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10759)
  AUTHORS   Zhang C, Ng DW, Lu J, Chen ZJ.
  TITLE     Roles of target site location and sequence complementarity in 
            trans-acting siRNA formation in Arabidopsis
  JOURNAL   Plant J. 69 (2), 217-226 (2012)
  PUBMED    21910773
REFERENCE   2  (bases 1 to 10759)
  AUTHORS   Zhang C, Chen ZJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-NOV-2010) Section of Molecular Cell and Developmental 
            Biology, The University of Texas at Austin, Austin, TX 78712, USA
REFERENCE   3  (bases 1 to 10759)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10759)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J."; 
            date: "2012"; volume: "69"; issue: "2"; pages: "217-226"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (03-NOV-2010) Section of Molecular Cell and Developmental Biology, 
            The University of Texas at Austin, Austin, TX 78712, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10759
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(37..1059)
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
     promoter        complement(1064..1444)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     promoter        1525..2196
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     CDS             2800..3516
                     /label=EGFP
                     /note="enhanced GFP"
     3'UTR           3529..3909
                     /note="contains octopine synthase terminator, a synthetic
                     poly linker, and CaMV 35S polyA signal"
     terminator      3925..4177
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    4199..4223
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             5523..6149
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             6586..7650
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      7719..7913
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    8257..8397
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8583..9171)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9261..10052)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     misc_feature    10477..10501
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(10579..10753)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"