LOCUS       40924_11456       10759 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Binary vector pCMIRT15, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10759)
  AUTHORS   Zhang C, Ng DW, Lu J, Chen ZJ.
  TITLE     Roles of target site location and sequence complementarity in 
            trans-acting siRNA formation in Arabidopsis
  JOURNAL   Plant J. 69 (2), 217-226 (2012)
  PUBMED    21910773
REFERENCE   2  (bases 1 to 10759)
  AUTHORS   Zhang C, Chen ZJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-NOV-2010) Section of Molecular Cell and Developmental 
            Biology, The University of Texas at Austin, Austin, TX 78712, USA
REFERENCE   3  (bases 1 to 10759)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10759)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J."; 
            date: "2012"; volume: "69"; issue: "2"; pages: "217-226"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (03-NOV-2010) Section of Molecular Cell and Developmental Biology, 
            The University of Texas at Austin, Austin, TX 78712, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10759
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(37..1059)
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
     promoter        complement(1064..1444)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     promoter        1525..2196
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     CDS             2800..3516
                     /label=EGFP
                     /note="enhanced GFP"
     3'UTR           3529..3909
                     /note="contains octopine synthase terminator, a synthetic
                     poly linker, and CaMV 35S polyA signal"
     terminator      3925..4177
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    4199..4223
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             5523..6149
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             6586..7650
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      7719..7913
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    8257..8397
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8583..9171)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9261..10052)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     misc_feature    10477..10501
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(10579..10753)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
ORIGIN
        1 cttgtcgatc gacagatccg gtcggcatct actctatttc tttgccctcg gacgagtgct
       61 ggggcgtcgg tttccactat cggcgagtac ttctacacag ccatcggtcc agacggccgc
      121 gcttctgcgg gcgatttgtg tacgcccgac agtcccggct ccggatcgga cgattgcgtc
      181 gcatcgaccc tgcgcccaag ctgcatcatc gaaattgccg tcaaccaagc tctgatagag
      241 ttggtcaaga ccaatgcgga gcatatacgc ccggagtcgt ggcgatcctg caagctccgg
      301 atgcctccgc tcgaagtagc gcgtctgctg ctccatacaa gccaaccacg gcctccagaa
      361 gaagatgttg gcgacctcgt attgggaatc cccgaacatc gcctcgctcc agtcaatgac
      421 cgctgttatg cggccattgt ccgtcaggac attgttggag ccgaaatccg cgtgcacgag
      481 gtgccggact tcggggcagt cctcggccca aagcatcagc tcatcgagag cctgcgcgac
      541 ggacgcactg acggtgtcgt ccatcacagt ttgccagtga tacacatggg gatcagcaat
      601 cgcgcatatg aaatcacgcc atgtagtgta ttgaccgatt ccttgcggtc cgaatgggcc
      661 gaacccgctc gtctggctaa gatcggccgc agcgatcgca tccatagcct ccgcgaccgg
      721 ttgtagaaca gcgggcagtt cggtttcagg caggtcttgc aacgtgacac cctgtgaacg
      781 gcgggagatg caataggtca ggctctcgct aaactcccca atgtcaagca cttccggaat
      841 cgggagcgcg gccgatgcaa agtgccgata aacataacga tctttgtaga aaccatcggc
      901 gcagctattt acccgcagga catatccacg ccctcctaca tcgaagctga aagcacgaga
      961 ttcttcgccc tccgagagct gcatcaggtc ggagacgctg tcgaactttt cgatcagaaa
     1021 cttctcgaca gacgtcgcgg tgagttcagg ctttttcatt gttcgatttg gtgtatcgag
     1081 attggttatg aaattcagat gctagtgtaa tgtattggta atttgggaag atataatagg
     1141 aagcaaggct atttatccat ttctgaaaag gcgaaatggc gtcaccgcga gcgtcacgcg
     1201 cattccgttc ttgctgtaaa gcgttgtttg gtacactttt gactagcgag gcttggcgtg
     1261 tcagcgtatc tattcaaaag tcgttaatgg ctgcggatca agaaaaagtt ggaatagaaa
     1321 cagaataccc gcgaaattca ggcccggttg ccatgtccta cacgccgaaa taaacgacca
     1381 aattagtaga aaaataaaaa ctgactcgga tacttacgtc acgtcttgcg cactgatttg
     1441 aaaagaattc aacatggtgg agcacgacac acttgtctac tccaaaaata tcaaagatac
     1501 agtctcagaa gaccaaaggg caattgagac ttttcaacaa agggtaatat ccggaaacct
     1561 cctcggattc cattgcccag ctatctgtca ctttattgtg aagatagtgg aaaaggaagg
     1621 tggctcctac aaatgccatc attgcgataa aggaaaggcc atcgttgaag atgcctctgc
     1681 cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaagacgt
     1741 tccaaccacg tcttcaaagc aagtggattg atgtgataac atggtggagc acgacacact
     1801 tgtctactcc aaaaatatca aagatacagt ctcagaagac caaagggcaa ttgagacttt
     1861 tcaacaaagg gtaatatccg gaaacctcct cggattccat tgcccagcta tctgtcactt
     1921 tattgtgaag atagtggaaa aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg
     1981 aaaggccatc gttgaagatg cctctgccga cagtggtccc aaagatggac ccccacccac
     2041 gaggagcatc gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag tggattgatg
     2101 tgatatctcc actgacgtaa gggatgacgc acaatcccac tatccttcgc aagacccttc
     2161 ctctatataa ggaagttcat ttcatttgga gaggacctcg aaattctcaa cacaacatat
     2221 acaaaacaaa cgaatctcaa gcaatcaagc attctacttc tattgcagca atttaaatca
     2281 tttcttttaa agcaaaagca attttctgaa aattttcacc atttacgaac gatagcaatg
     2341 gaatccaccg gaaaagtgaa gaaggctttc ggaggaagaa aaccacctgg tgccccaaaa
     2401 accaaatcgg tttcgaaatc gatgaaagcc ggtcttcaat tcccagtggg aagaatcact
     2461 cgtttcctga agaaaggacg atacgctcag agacttggtg gtggtgctcc ggtttacatg
     2521 gccgccgttc ttgaatacct cgccgcagaa gttctggagc ttgctggtaa cgctgcgaga
     2581 gataacaaga aatcaaggat aattccgagg catcttcttc tcgcgataag gaacgatgaa
     2641 gaattgggga aacttctgag tggtgtcaca atcgctcacg gtggtgtttt gcctaacatc
     2701 aactctgttc tattgcctaa gaagtctgcc actaaaccag ctgaagaaaa ggctaccaaa
     2761 tcaccagtca agtctccaaa gaaagctcac gtggggccca tggtgagcaa gggcgaggag
     2821 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
     2881 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
     2941 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cttcacctac
     3001 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
     3061 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
     3121 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
     3181 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac
     3241 agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag
     3301 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
     3361 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc
     3421 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
     3481 gccgggatca ctcacggcat ggacgagctg tacaagggga tcctctagac ccgggactag
     3541 tccctagagt cctgctttaa tgagatatgc gagacgccta tgatcgcatg atatttgctt
     3601 tcaattctgt tgtgcacgtt gctcgagagc gctgtcgacc tgcaggcatg caagcttgag
     3661 ctcggtaccg ttaccagatc tgtccgcaaa aatcaccagt ctctctctac aaatctatct
     3721 ctctctattt ttctccagaa taatgtgtga gtagttccca gataagggaa ttagggttct
     3781 tatagggttt cgctcatgtg ttgagcatat aagaaaccct tagtatgtat ttgtatttgt
     3841 aaaatacttc tatcaataaa atttctaatt cctaaaacca aaatccagtg accctcaggc
     3901 ctggtgacca gctcgaattt ccccgatcgt tcaaacattt ggcaataaag tttcttaaga
     3961 ttgaatcctg ttgccggtct tgcgatgatt atcatataat ttctgttgaa ttacgttaag
     4021 catgtaataa ttaacatgta atgcatgacg ttatttatga gatgggtttt tatgattaga
     4081 gtcccgcaat tatacattta atacgcgata gaaaacaaaa tatagcgcgc aaactaggat
     4141 aaattatcgc gcgcggtgtc atctatgtta ctagatcggg aattaaacta tcagtgtttg
     4201 acaggatata ttggcgggta aacctaagag aaaagagcgt ttattagaat aacggatatt
     4261 taaaagggcg tgaaaaggtt tatccgttcg tccatttgta tgtgcatgcc aaccacaggg
     4321 ttcccctcgg gatcaaagta ctttgatcca acccctccgc tgctatagtg cagtcggctt
     4381 ctgacgttca gtgcagccgt cttctgaaaa cgacatgtcg cacaagtcct aagttacgcg
     4441 acaggctgcc gccctgccct tttcctggcg ttttcttgtc gcgtgtttta gtcgcataaa
     4501 gtagaatact tgcgactaga accggagaca ttacgccatg aacaagagcg ccgccgctgg
     4561 cctgctgggc tatgcccgcg tcagcaccga cgaccaggac ttgaccaacc aacgggccga
     4621 actgcacgcg gccggctgca ccaagctgtt ttccgagaag atcaccggca ccaggcgcga
     4681 ccgcccggag ctggccagga tgcttgacca cctacgccct ggcgacgttg tgacagtgac
     4741 caggctagac cgcctggccc gcagcacccg cgacctactg gacattgccg agcgcatcca
     4801 ggaggccggc gcgggcctgc gtagcctggc agagccgtgg gccgacacca ccacgccggc
     4861 cggccgcatg gtgttgaccg tgttcgccgg cattgccgag ttcgagcgtt ccctaatcat
     4921 cgaccgcacc cggagcgggc gcgaggccgc caaggcccga ggcgtgaagt ttggcccccg
     4981 ccctaccctc accccggcac agatcgcgca cgcccgcgag ctgatcgacc aggaaggccg
     5041 caccgtgaaa gaggcggctg cactgcttgg cgtgcatcgc tcgaccctgt accgcgcact
     5101 tgagcgcagc gaggaagtga cgcccaccga ggccaggcgg cgcggtgcct tccgtgagga
     5161 cgcattgacc gaggccgacg ccctggcggc cgccgagaat gaacgccaag aggaacaagc
     5221 atgaaaccgc accaggacgg ccaggacgaa ccgtttttca ttaccgaaga gatcgaggcg
     5281 gagatgatcg cggccgggta cgtgttcgag ccgcccgcgc acgtctcaac cgtgcggctg
     5341 catgaaatcc tggccggttt gtctgatgcc aagctggcgg cctggccggc cagcttggcc
     5401 gctgaagaaa ccgagcgccg ccgtctaaaa aggtgatgtg tatttgagta aaacagcttg
     5461 cgtcatgcgg tcgctgcgta tatgatgcga tgagtaaata aacaaatacg caaggggaac
     5521 gcatgaaggt tatcgctgta cttaaccaga aaggcgggtc aggcaagacg accatcgcaa
     5581 cccatctagc ccgcgccctg caactcgccg gggccgatgt tctgttagtc gattccgatc
     5641 cccagggcag tgcccgcgat tgggcggccg tgcgggaaga tcaaccgcta accgttgtcg
     5701 gcatcgaccg cccgacgatt gaccgcgacg tgaaggccat cggccggcgc gacttcgtag
     5761 tgatcgacgg agcgccccag gcggcggact tggctgtgtc cgcgatcaag gcagccgact
     5821 tcgtgctgat tccggtgcag ccaagccctt acgacatatg ggccaccgcc gacctggtgg
     5881 agctggttaa gcagcgcatt gaggtcacgg atggaaggct acaagcggcc tttgtcgtgt
     5941 cgcgggcgat caaaggcacg cgcatcggcg gtgaggttgc cgaggcgctg gccgggtacg
     6001 agctgcccat tcttgagtcc cgtatcacgc agcgcgtgag ctacccaggc actgccgccg
     6061 ccggcacaac cgttcttgaa tcagaacccg agggcgacgc tgcccgcgag gtccaggcgc
     6121 tggccgctga aattaaatca aaactcattt gagttaatga ggtaaagaga aaatgagcaa
     6181 aagcacaaac acgctaagtg ccggccgtcc gagcgcacgc agcagcaagg ctgcaacgtt
     6241 ggccagcctg gcagacacgc cagccatgaa gcgggtcaac tttcagttgc cggcggagga
     6301 tcacaccaag ctgaagatgt acgcggtacg ccaaggcaag accattaccg agctgctatc
     6361 tgaatacatc gcgcagctac cagagtaaat gagcaaatga ataaatgagt agatgaattt
     6421 tagcggctaa aggaggcggc atggaaaatc aagaacaacc aggcaccgac gccgtggaat
     6481 gccccatgtg tggaggaacg ggcggttggc caggcgtaag cggctgggtt gtctgccggc
     6541 cctgcaatgg cactggaacc cccaagcccg aggaatcggc gtgacggtcg caaaccatcc
     6601 ggcccggtac aaatcggcgc ggcgctgggt gatgacctgg tggagaagtt gaaggccgcg
     6661 caggccgccc agcggcaacg catcgaggca gaagcacgcc ccggtgaatc gtggcaagcg
     6721 gccgctgatc gaatccgcaa agaatcccgg caaccgccgg cagccggtgc gccgtcgatt
     6781 aggaagccgc ccaagggcga cgagcaacca gattttttcg ttccgatgct ctatgacgtg
     6841 ggcacccgcg atagtcgcag catcatggac gtggccgttt tccgtctgtc gaagcgtgac
     6901 cgacgagctg gcgaggtgat ccgctacgag cttccagacg ggcacgtaga ggtttccgca
     6961 gggccggccg gcatggccag tgtgtgggat tacgacctgg tactgatggc ggtttcccat
     7021 ctaaccgaat ccatgaaccg ataccgggaa gggaagggag acaagcccgg ccgcgtgttc
     7081 cgtccacacg ttgcggacgt actcaagttc tgccggcgag ccgatggcgg aaagcagaaa
     7141 gacgacctgg tagaaacctg cattcggtta aacaccacgc acgttgccat gcagcgtacg
     7201 aagaaggcca agaacggccg cctggtgacg gtatccgagg gtgaagcctt gattagccgc
     7261 tacaagatcg taaagagcga aaccgggcgg ccggagtaca tcgagatcga gctagctgat
     7321 tggatgtacc gcgagatcac agaaggcaag aacccggacg tgctgacggt tcaccccgat
     7381 tactttttga tcgatcccgg catcggccgt tttctctacc gcctggcacg ccgcgccgca
     7441 ggcaaggcag aagccagatg gttgttcaag acgatctacg aacgcagtgg cagcgccgga
     7501 gagttcaaga agttctgttt caccgtgcgc aagctgatcg ggtcaaatga cctgccggag
     7561 tacgatttga aggaggaggc ggggcaggct ggcccgatcc tagtcatgcg ctaccgcaac
     7621 ctgatcgagg gcgaagcatc cgccggttcc taatgtacgg agcagatgct agggcaaatt
     7681 gccctagcag gggaaaaagg tcgaaaaggt ctctttcctg tggatagcac gtacattggg
     7741 aacccaaagc cgtacattgg gaaccggaac ccgtacattg ggaacccaaa gccgtacatt
     7801 gggaaccggt cacacatgta agtgactgat ataaaagaga aaaaaggcga tttttccgcc
     7861 taaaactctt taaaacttat taaaactctt aaaacccgcc tggcctgtgc ataactgtct
     7921 ggccagcgca cagccgaaga gctgcaaaaa gcgcctaccc ttcggtcgct gcgctcccta
     7981 cgccccgccg cttcgcgtcg gcctatcgcg gccgctggcc gctcaaaaat ggctggccta
     8041 cggccaggca atctaccagg gcgcggacaa gccgcgccgt cgccactcga ccgccggcgc
     8101 ccacatcaag gcaccctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
     8161 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
     8221 tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc agtcacgtag
     8281 cgatagcgga gtgtatactg gcttaactat gcggcatcag agcagattgt actgagagtg
     8341 caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc
     8401 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     8461 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     8521 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     8581 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     8641 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     8701 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     8761 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     8821 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     8881 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     8941 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     9001 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
     9061 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     9121 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     9181 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     9241 gtcatgcatt ctaggtacta aaacaattca tccagtaaaa tataatattt tattttctcc
     9301 caatcaggct tgatccccag taagtcaaaa aatagctcga catactgttc ttccccgata
     9361 tcctccctga tcgaccggac gcagaaggca atgtcatacc acttgtccgc cctgccgctt
     9421 ctcccaagat caataaagcc acttactttg ccatctttca caaagatgtt gctgtctccc
     9481 aggtcgccgt gggaaaagac aagttcctct tcgggctttt ccgtctttaa aaaatcatac
     9541 agctcgcgcg gatctttaaa tggagtgtct tcttcccagt tttcgcaatc cacatcggcc
     9601 agatcgttat tcagtaagta atccaattcg gctaagcggc tgtctaagct attcgtatag
     9661 ggacaatccg atatgtcgat ggagtgaaag agcctgatgc actccgcata cagctcgata
     9721 atcttttcag ggctttgttc atcttcatac tcttccgagc aaaggacgcc atcggcctca
     9781 ctcatgagca gattgctcca gccatcatgc cgttcaaagt gcaggacctt tggaacaggc
     9841 agctttcctt ccagccatag catcatgtcc ttttcccgtt ccacatcata ggtggtccct
     9901 ttataccggc tgtccgtcat ttttaaatat aggttttcat tttctcccac cagcttatat
     9961 accttagcag gagacattcc ttccgtatct tttacgcagc ggtatttttc gatcagtttt
    10021 ttcaattccg gtgatattct cattttagcc atttattatt tccttcctct tttctacagt
    10081 atttaaagat accccaagaa gctaattata acaagacgaa ctccaattca ctgttccttg
    10141 cattctaaaa ccttaaatac cagaaaacag ctttttcaaa gttgttttca aagttggcgt
    10201 ataacatagt atcgacggag ccgattttga aaccgcggtg atcacaggca gcaacgctct
    10261 gtcatcgtta caatcaacat gctaccctcc gcgagatcat ccgtgtttca aacccggcag
    10321 cttagttgcc gttcttccga atagcatcgg taacatgagc aaagtctgcc gccttacaac
    10381 ggctctcccg ctgacgccgt cccggactga tgggctgcct gtatcgagtg gtgattttgt
    10441 gccgagctgc cggtcgggga gctgttggct ggctggtggc aggatatatt gtggtgtaaa
    10501 caaattgacg cttagacaac ttaataacac attgcggacg tttttaatgt actgaattaa
    10561 cgccgaatta attcggggga tctggatttt agtactggat tttggtttta ggaattagaa
    10621 attttattga tagaagtatt ttacaaatac aaatacatac taagggtttc ttatatgctc
    10681 aacacatgag cgaaacccta taggaaccct aattccctta tctgggaact actcacacat
    10741 tattatggag aaactcgac
//
