pCloneNat1 vector (V008446)

LOCUS       40924_11301        3707 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCloneNat1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3707)
  AUTHORS   Gregan J, Rabitsch KP, Rumpf C, Nasmyth K.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2006) Chromosome Biology, University of Vienna, 
            Dr. Bohrgasse 1, Vienna 1030, Austria
REFERENCE   2  (bases 1 to 3707)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 3707)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (02-NOV-2006) Chromosome Biology, University of Vienna, Dr. 
            Bohrgasse 1, Vienna 1030, Austria"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3707
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     gene            115..1237
                     /label=natMX6
                     /note="yeast selectable marker conferring nourseothricin 
                     resistance"
     promoter        complement(1345..1363)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(1621..2209)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2383..3240)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3241..3345)
                     /label=AmpR promoter
     promoter        3691..3707
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"