LOCUS       40924_11301        3707 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCloneNat1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3707)
  AUTHORS   Gregan J, Rabitsch KP, Rumpf C, Nasmyth K.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2006) Chromosome Biology, University of Vienna, 
            Dr. Bohrgasse 1, Vienna 1030, Austria
REFERENCE   2  (bases 1 to 3707)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 3707)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (02-NOV-2006) Chromosome Biology, University of Vienna, Dr. 
            Bohrgasse 1, Vienna 1030, Austria"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3707
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     gene            115..1237
                     /label=natMX6
                     /note="yeast selectable marker conferring nourseothricin 
                     resistance"
     promoter        complement(1345..1363)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(1621..2209)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2383..3240)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3241..3345)
                     /label=AmpR promoter
     promoter        3691..3707
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gaacgcggcc gccagctgaa gcttctcgag tgtacaccat ggggatcccc gggttaatta
       61 aggcgcgcca gatctgttta gcttgcctcg tccccgccgg gtcacccggc cagcgacatg
      121 gaggcccaga ataccctcct tgacagtctt gacgtgcgca gctcaggggc atgatgtgac
      181 tgtcgcccgt acatttagcc catacatccc catgtataat catttgcatc catacatttt
      241 gatggccgca cggcgcgaag caaaaattac ggctcctcgc tgcagacctg cgagcaggga
      301 aacgctcccc tcacagacgc gttgaattgt ccccacgccg cgcccctgta gagaaatata
      361 aaaggttagg atttgccact gaggttcttc tttcatatac ttccttttaa aatcttgcta
      421 ggatacagtt ctcacatcac atccgaacat aaacaaccat gggtaccact cttgacgaca
      481 cggcttaccg gtaccgcacc agtgtcccgg gggacgccga ggccatcgag gcactggatg
      541 ggtccttcac caccgacacc gtcttccgcg tcaccgccac cggggacggc ttcaccctgc
      601 gggaggtgcc ggtggacccg cccctgacca aggtgttccc cgacgacgaa tcggacgacg
      661 aatcggacga cggggaggac ggcgacccgg actcccggac gttcgtcgcg tacggggacg
      721 acggcgacct ggcgggcttc gtggtcatct cgtactcggc gtggaaccgc cggctgaccg
      781 tcgaggacat cgaggtcgcc ccggagcacc gggggcacgg ggtcgggcgc gcgttgatgg
      841 ggctcgcgac ggagttcgcc ggcgagcggg gcgccgggca cctctggctg gaggtcacca
      901 acgtcaacgc accggcgatc cacgcgtacc ggcggatggg gttcaccctc tgcggcctgg
      961 acaccgccct gtacgacggc accgcctcgg acggcgagcg gcaggcgctc tacatgagca
     1021 tgccctgccc ctaatcagta ctgacaataa aaagattctt gttttcaaga acttgtcatt
     1081 tgtatagttt ttttatattg tagttgttct attttaatca aatgttagcg tgatttatat
     1141 tttttttcgc ctcgacatca tctgcccaga tgcgaagtta agtgcgcaga aagtaatatc
     1201 atgcgtcaat cgtatgtgaa tgctggtcgc tatactgctg tcgattcgat actaacgccg
     1261 ccatccagtg tcgaaaacga gctcgaattc atcgatgata tcagatccac tagtggccta
     1321 tgcggccgcg gatctgccgg tctccctata gtgagtcgta ttaatttcga taagccaggt
     1381 taacctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     1441 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     1501 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     1561 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     1621 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     1681 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     1741 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     1801 cgtggcgctt tctcaatgct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     1861 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     1921 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     1981 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     2041 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
     2101 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     2161 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     2221 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     2281 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     2341 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     2401 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     2461 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     2521 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     2581 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     2641 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     2701 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     2761 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     2821 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     2881 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     2941 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     3001 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     3061 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     3121 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     3181 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     3241 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     3301 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     3361 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
     3421 tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
     3481 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
     3541 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga
     3601 gcagattgta ctgagagtgc accatatgga catattgtcg ttagaacgcg gctacaatta
     3661 atacataacc ttatgtatca tacacatacg atttaggtga cactata
//
