pCLEAN-G265 vector (V008454)

LOCUS       V008454                 8114 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008454
VERSION     V008454
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8114)
  AUTHORS   Thole V, Worland B, Snape JW, Vain P.
  TITLE     The pCLEAN dual binary vector system for Agrobacterium-mediated
            plant transformation
  JOURNAL   Plant Physiol. 145 (4), 1211-1219 (2007)
   PUBMED   17932303
REFERENCE   2  (bases 1 to 8114)
  AUTHORS   Thole V, Vain P.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2013) John Innes Centre, Norwich Research Park,
            Norwich, Norfolk NR4 7UH, United Kingdom
REFERENCE   3  (bases 1 to 8114)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8114)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Plant
            Physiol."; date: "2007"; volume: "145"; issue: "4"; pages:
            "1211-1219"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (13-DEC-2013) John Innes Centre, Norwich Research Park, Norwich,
            Norfolk NR4 7UH, United Kingdom"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8114
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(61..649)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(823..1635)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      1926..2361
                     /label="pSa ori"
                     /note="origin of replication from bacterial plasmid pSa"
     misc_feature    2508..2532
                     /label="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
     misc_feature    2533..2558
                     /note="spacer sequence separating left border No.1 and No.2
                     of the double left border sequence"
     misc_feature    2559..2583
                     /note="nopaline-type T-DNA left border sequence No.2
                     belonging to a double left border sequence separated by a
                     spacer sequence from left border No.1"
     terminator      complement(2607..2859)
                     /label="NOS terminator"
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(2877..3425)
                     /label="BlpR"
                     /note="phosphinothricin acetyltransferase"
     intron          complement(3458..3991)
                     /note="adh1-intron 1; first intron of maize alcohol
                     dehydrogenase 1 (adh1-1S)"
     promoter        complement(4022..4366)
                     /label="CaMV 35S promoter"
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     regulatory      complement(4466..4758)
                     /note="SPA-ter; soybean-derived poly(A) signal"
                     /regulatory_class="terminator"
     promoter        complement(4803..6799)
                     /label="Ubi promoter"
                     /note="maize polyubiquitin gene promoter"
     misc_feature    6849..6952
                     /note="UniBox2; synthetic unique sequence containing
                     forward and reverse primer sequences"
     misc_feature    6959..6983
                     /label="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     misc_feature    6984..7007
                     /label="right border overdrive sequence"
                     /note="right border overdrive sequence"
     CDS             7211..8011
                     /gene="virG"
                     /label="Regulatory protein VirG"
                     /note="Regulatory protein VirG from Agrobacterium
                     tumefaciens (strain 15955). Accession#: P62722"