LOCUS       V008454                 8114 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008454
VERSION     V008454
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8114)
  AUTHORS   Thole V, Worland B, Snape JW, Vain P.
  TITLE     The pCLEAN dual binary vector system for Agrobacterium-mediated
            plant transformation
  JOURNAL   Plant Physiol. 145 (4), 1211-1219 (2007)
   PUBMED   17932303
REFERENCE   2  (bases 1 to 8114)
  AUTHORS   Thole V, Vain P.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2013) John Innes Centre, Norwich Research Park,
            Norwich, Norfolk NR4 7UH, United Kingdom
REFERENCE   3  (bases 1 to 8114)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8114)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Plant
            Physiol."; date: "2007"; volume: "145"; issue: "4"; pages:
            "1211-1219"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (13-DEC-2013) John Innes Centre, Norwich Research Park, Norwich,
            Norfolk NR4 7UH, United Kingdom"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8114
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(61..649)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(823..1635)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      1926..2361
                     /label="pSa ori"
                     /note="origin of replication from bacterial plasmid pSa"
     misc_feature    2508..2532
                     /label="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
     misc_feature    2533..2558
                     /note="spacer sequence separating left border No.1 and No.2
                     of the double left border sequence"
     misc_feature    2559..2583
                     /note="nopaline-type T-DNA left border sequence No.2
                     belonging to a double left border sequence separated by a
                     spacer sequence from left border No.1"
     terminator      complement(2607..2859)
                     /label="NOS terminator"
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(2877..3425)
                     /label="BlpR"
                     /note="phosphinothricin acetyltransferase"
     intron          complement(3458..3991)
                     /note="adh1-intron 1; first intron of maize alcohol
                     dehydrogenase 1 (adh1-1S)"
     promoter        complement(4022..4366)
                     /label="CaMV 35S promoter"
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     regulatory      complement(4466..4758)
                     /note="SPA-ter; soybean-derived poly(A) signal"
                     /regulatory_class="terminator"
     promoter        complement(4803..6799)
                     /label="Ubi promoter"
                     /note="maize polyubiquitin gene promoter"
     misc_feature    6849..6952
                     /note="UniBox2; synthetic unique sequence containing
                     forward and reverse primer sequences"
     misc_feature    6959..6983
                     /label="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     misc_feature    6984..7007
                     /label="right border overdrive sequence"
                     /note="right border overdrive sequence"
     CDS             7211..8011
                     /gene="virG"
                     /label="Regulatory protein VirG"
                     /note="Regulatory protein VirG from Agrobacterium
                     tumefaciens (strain 15955). Accession#: P62722"
ORIGIN
        1 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
       61 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
      121 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      181 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      241 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      301 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      361 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      421 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      481 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
      541 cttcggaaga agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
      601 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
      661 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
      721 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
      781 atcaatctaa agtatatatg tgtaacattg gtctagtgat tagaaaaact catcgagcat
      841 caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg
      901 tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta
      961 tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa
     1021 aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa
     1081 aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa
     1141 atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac
     1201 gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac
     1261 tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc
     1321 tgttttccct gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg
     1381 cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt
     1441 aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt
     1501 cccatacaat cggtagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata
     1561 cccatataaa tcagcatcca tgttggaatt taatcgcggc cttgagcaag acgtttcccg
     1621 ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt
     1681 tcatgatgat atatttttat cttgtgcaat gtaacatcag agattttgag acacaacgtg
     1741 gctttgttga ataaatcgaa cttttgctga gttgaaggat cagatcacgc atcttcccga
     1801 caacgcagac cgttccgtgg caaagcaaaa gttcaaaatc accaactggt ccacctacaa
     1861 caaagctctc atcaaccgtg gctccctcac tttctggctg gatgatgggg cgattcaggc
     1921 gatccccatc caacagcccg ccgtcgagcg ggctttttta tccccggaag cctgtggata
     1981 gagggtagtt atccacgtga aaccgctaat gccccgcaaa gccttgattc acggggcttt
     2041 ccggcccgct ccaaaaacta tccacgtgaa atcgctaatc agggtacgtg aaatcgctaa
     2101 tcggagtacg tgaaatcgct aataaggtca cgtgaaatcg ctaatcaaaa aggcacgtga
     2161 gaacgctaat agccctttca gatcaacagc ttgcaaacac ccctcgctcc ggcaagtagt
     2221 tacagcaagt agtatgttca attagctttt caattatgaa tatatatatc aattattggt
     2281 cgcccttggc ttgtggacaa tgcgctacgc gcaccggctc cgcccgtgga caaccgcaag
     2341 cggttgccca ccgtcgagcg cctttgccca caacccggcg gccggccgca acagatcgtt
     2401 ttataaattt ttttttttga aaaagaaaaa gcccgaaagg cggcaacctc tcgggcttct
     2461 ggatttccga tccccggaat tagatccgtt taaactacgt aagatcttgg caggatatat
     2521 tgtggtgtaa acgttcctgc ggcggtcgag atggatcttg gcaggatata ttgtggtgta
     2581 aacgttcctg cggccgctta ttaaacgatc tagtaacata gatgacaccg cgcgcgataa
     2641 tttatcctag tttgcgcgct atattttgtt ttctatcgcg tattaaatgt ataattgcgg
     2701 gactctaatc ataaaaaccc atctcataaa taacgtcatg cattacatgt taattattac
     2761 atgcttaacg taattcaaca gaaattatat gataatcatc gcaagaccgg caacaggatt
     2821 caatcttaag aaactttatt gccaaatgtt tgaacgatct gcaggtcgac ggatcagatc
     2881 tcggtgacgg gcaggaccgg acggggcggt accggcaggc tgaagtccag ctgccagaaa
     2941 cccacgtcat gccagttccc gtgcttgaag ccggccgccc gcagcatgcc gcggggggca
     3001 tatccgagcg cctcgtgcat gcgcacgctc gggtcgttgg gcagcccgat gacagcgacc
     3061 acgctcttga agccctgtgc ctccagggac ttcagcaggt gggtgtagag cgtggagccc
     3121 agtcccgtcc gctggtggcg gggggagacg tacacggtcg actcggccgt ccagtcgtag
     3181 gcgttgcgtg ccttccaggg gcccgcgtag gcgatgccgg cgacctcgcc gtccacctcg
     3241 gcgacgagcc agggatagcg ctcccgcaga cggacgaggt cgtccgtcca ctcctgcggt
     3301 tcctgcggct cggtacggaa gttgaccgtg cttgtctcga tgtagtggtt gacgatggtg
     3361 cagaccgccg gcatgtccgc ctcggtggca cggcggatgt cggccgggcg tcgttctggg
     3421 ctcatggtta cttcctaatc gatggatccg tccgcagctg cacgggtcca ggaaagcaat
     3481 cgcatagtca agctaaatca tcaagatgca aacttttcgc ccttgctaaa cacggtaaaa
     3541 ttcgaatgga catgtgtgga gcagcaaagg ccttacgtcc gagaaacagg gccactcaac
     3601 gagttagtta aattcaaaga aagaaacgcc tccttgcaag ttgcaacatt cttagatcat
     3661 actgatgaaa atgacgtctt tcattaaaga acagggaaga tagatctttg ctcaatatcg
     3721 tatgatgtgt tcagccagac tgtcggatgg accacacggt aatagcagtg ctggacgatg
     3781 ttacatcgag aaagattact agccttttca tgggagtgaa ggatataaaa gaaataagtt
     3841 caccacgatt gcaggatagc atacaagatc agcgccactg cggcactgtt catcgaaaaa
     3901 aaaactgtgg acgaagcttt ccccaaaatt actcaacgaa tcataaacca agattagtca
     3961 gatcaagaga cagaggagaa acaaggcgga cctttgcact tgatccgtcg acctgcaggt
     4021 cgtcctctcc aaatgaaatg aacttcctta tatagaggaa gggtcttgcg aaggatagtg
     4081 ggattgtgcg tcatccctta cgtcagtgga gatatcacat caatccactt gctttgaaga
     4141 cgtggttgga acgtcttctt tttccacgat gctcctcgtg ggtgggggtc catctttggg
     4201 accactgtcg gcagaggcat cttgaacgat agcctttcct ttatcgcaat gatggcattt
     4261 gtaggtgcca ccttcctttt ctactgtcct tttgatgaag tgacagatag ctgggcaatg
     4321 gaatccgagg aggtttcccg atattaccct ttgttgaaaa gtctcaatag ccctttggtc
     4381 ttctgagact gtatctttga tattcttgga gtagacgaga gtgtcgtgct ccaccatgtt
     4441 gacttaatta ataaaaggcc gtacggttaa ccatcattta gtgacacaat taacttaaat
     4501 ttagaagaga ctaatttcaa cccacaagat ggaaatgcaa ggacaatggg ttcttgaagt
     4561 tatttggaaa caaagcaaca caacacaata gcttattcat tagttctcat ccattaattt
     4621 ccattgaagt ccgaaaacag aaaccaaaaa tacaggatga tgaaatctca taaagcaaaa
     4681 acaaatggga atgtagttta agtacaacaa acaaagtggg gtggctacag atgctttgca
     4741 aatttttcaa tgacttacgg taccagctgg cgcgccggcc aattaggccg gttaccaagc
     4801 ttctgcagaa gtaacaccaa acaacagggt gagcatcgac aaaagaaaca gtaccaagca
     4861 aataaatagc gtatgaaggc agggctaaaa aaatccacat atagctgctg catatgccat
     4921 catccaagta tatcaagatc aaaataatta taaaacatac ttgtttatta taatagatag
     4981 gtactcaagg ttagagcata tgaatagatg ctgcatatgc catcatgtat atgcatcagt
     5041 aaaacccaca tcaacatgta tacctatcct agatcgatat ttccatccat cttaaactcg
     5101 taactatgaa gatgtatgac acacacatac agttccaaaa ttaataaata caccaggtag
     5161 tttgaaacag tattctactc cgatctagaa cgaatgaacg accgcccaac cacaccacat
     5221 catcacaacc aagcgaacaa aaagcatctc tgtatatgca tcagtaaaac ccgcatcaac
     5281 atgtatacct atcctagatc gatatttcca tccatcatct tcaattcgta actatgaata
     5341 tgtatggcac acacatacag atccaaaatt aataaatcca ccaggtagtt tgaaacagaa
     5401 ttaattctac tccgatctag aacgaccgcc caaccagacc acatcatcac aaccaagaca
     5461 aaaaaaagca tgaaaagatg acccgacaaa caagtgcacg gcatatattg aaataaagga
     5521 aaagggcaaa ccaaacccta tgcaacgaaa caaaaaaaat catgaaatcg atcccgtctg
     5581 cggaacggct agagccatcc caggattccc caaagagaaa cactggcaag ttagcaatca
     5641 gaacgtgtct gacgtacagg tcgcatccgt gtacgaacgc tagcagcacg gatctaacac
     5701 aaacacggat ctaacacaaa catgaacaga agtagaacta ccgggcccta accatggacc
     5761 ggaacgccga tctagagaag gtagagaggg gggggggggg aggacgagcg gcgtaccttg
     5821 aagcggaggt gccgacgggt ggatttgggg gagatctggt tgtgtgtgtg tgcgctccga
     5881 acaacacgag gttggggaaa gagggtgtgg agggggtgtc tatttattac ggcgggcgag
     5941 gaagggaaag cgaaggagcg gtgggaaagg aatcccccgt agctgccggt gccgtgagag
     6001 gaggaggagg ccgcctgccg tgccggctca cgtctgccgc tccgccacgc aatttctgga
     6061 tgccgacagc ggagcaagtc caacggtgga gcggaactct cgagaggggt ccagaggcag
     6121 cgacagagat gccgtgccgt ctgcttcgct tggcccgacg cgacgctgct ggttcgctgg
     6181 ttggtgtccg ttagactcgt cgacggcgtt taacaggctg gcattatcta ctcgaaacaa
     6241 gaaaaatgtt tccttagttt ttttaatttc ttaaagggta tttgtttaat ttttagtcac
     6301 tttattttat tctattttat atctaaatta ttaaataaaa aaactaaaat agagttttag
     6361 ttttcttaat ttagaggcta aaatagaata aaatagatgt actaaaaaaa ttagtctata
     6421 aaaaccatta accctaaacc ctaaatggat gtactaataa aatggatgaa gtattatata
     6481 ggtgaagcta tttgcaaaaa aaaaggagaa cacatgcaca ctaaaaagat aaaactgtag
     6541 agtcctgttg tcaaaatact caattgtcct ttagaccatg tctaactgtt catttatatg
     6601 attctctaaa acactgatat tattgtagta ctatagatta tattattcgt agagtaaagt
     6661 ttaaatatat gtataaagat agataaactg cacttcaaac aagtgtgaca aaaaaaatat
     6721 gtggtaattt tttataactt agacatgcaa tgctcattat ctctagagag gggcacgacc
     6781 gggtcacgct gcactgcagg aattcaagct ggcatcgaga ttcaaactag tctcgagtcg
     6841 acaggccttt ctaacctgtc gaggatacgt gataagcggg agcttagcgg agctcgttgc
     6901 aatcaaagct cgatacgtcg agacttagag gatctacgac agtccacaga acaggccttg
     6961 acaggatata ttggcgggta aactaagtcg ctgtatgtgt ttgtttgttt aaacccggga
     7021 ggctcgcggc ggacgcacga cgccggggcg agaccatagg cgatctccta aatcaatagt
     7081 agctgtaacc tcgaagcgtt tcacttgtaa caacgattga gaatttttgt cataaaattg
     7141 aaatacttgg ttcgcatttt tgtcatccgc ggtcagccgc aattctgacg aactgcccat
     7201 ttagctggag atgattgtac atccttcacg tgaaaatttc tcaagcgctg tgaacaaggg
     7261 ttcagatttt agattgaaag gtgagccgtt gaaacacgtt cttcttgtcg atgacgacgt
     7321 cgctatgcgg catcttatta ttgaatacct tacgatccac gccttcaaag tgaccgcggt
     7381 agccgacagc acccagttca caagagtact ctcttccgcg acggtcgatg tcgtggttgt
     7441 tgatctaaat ttaggtcgtg aagatgggct cgagatcgtt cgtaatctgg cggcaaagtc
     7501 tgatattcca atcataatta tcagtggcga ccgccttgag gagacggata aagttgttgc
     7561 actcgagcta ggagcaagtg attttatcgc taagccgttc agtatcagag agtttctagc
     7621 acgcattcgg gttgccttgc gcgtgcgccc caacgttgtc cgctccaaag accgacggtc
     7681 tttttgtttt actgactgga cacttaatct caggcaacgt cgcttgatgt ccgaagctgg
     7741 cggtgaggtg aaacttacgg caggtgagtt caatcttctc ctcgcgtttt tagagaaacc
     7801 ccgcgacgtt ctatcgcgcg agcaacttct cattgccagt cgagtacgcg acgaggaggt
     7861 ttatgacagg agtatagatg ttctcatttt gaggctgcgc cgcaaacttg aggcggatcc
     7921 gtcaagccct caactgataa aaacagcaag aggtgccggt tatttctttg acgcggacgt
     7981 gcaggtttcg cacgggggga cgatggcagc ctgagccaat tgcatttgcc tcttaattat
     8041 ctggctcaaa gggtgactga ggagtaagcg atgtgcccat cacactgccc gggcatgcaa
     8101 gctgatctgg atct
//
