pCLEAN-G185 vector (V008455)

LOCUS       V008455                 3793 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008455
VERSION     V008455
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3793)
  AUTHORS   Thole V, Worland B, Snape JW, Vain P.
  TITLE     The pCLEAN dual binary vector system for Agrobacterium-mediated
            plant transformation
  JOURNAL   Plant Physiol. 145 (4), 1211-1219 (2007)
   PUBMED   17932303
REFERENCE   2  (bases 1 to 3793)
  AUTHORS   Thole V, Vain P.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2007) Department of Crop Genetics, John Innes
            Centre, Colney Lane, Norwich NR4 7UH, UK
REFERENCE   3  (bases 1 to 3793)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3793)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant
            Physiol."; date: "2007"; volume: "145"; issue: "4"; pages:
            "1211-1219"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (30-SEP-2007) Department of Crop Genetics, John Innes Centre, Colney
            Lane, Norwich NR4 7UH, UK"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3793
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(61..649)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(823..1635)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      1926..2361
                     /label="pSa ori"
                     /note="origin of replication from bacterial plasmid pSa"
     misc_feature    2508..2532
                     /label="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
     misc_feature    2533..2558
                     /note="26-nt long spacer sequence separating left border
                     No. 1 and No. 2 of the double left border sequence (Thole
                     et al., 2007)"
     misc_feature    2559..2583
                     /note="T-DNA left border No. 2 sequence belonging to a
                     double left border sequence separated by a 26-nt long
                     spacer sequence from left border No. 1 sequence (Thole et
                     al., 2007); left border No. 2 is a 25-nt long consensus
                     sequence of the nopaline vector pTiT37 (Slightom et al.,
                     (1985) EMBO J 4:3069-3077)"
     misc_feature    2584..2635
                     /note="Inner T-DNA region containing 52 nt-long multiple
                     cloning site with NotI, PacI, SfiI, BsiWI, ApaI, XhoI and
                     SalI restriction sites"
     misc_feature    2636..2660
                     /label="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     misc_feature    2661..2684
                     /note="right border overdrive sequence (Peralta et al.
                     (1986) EMBO J 5: 1137-1142)"
     CDS             2894..3694
                     /gene="virG"
                     /label="Regulatory protein VirG"
                     /note="Regulatory protein VirG from Agrobacterium
                     tumefaciens (strain 15955). Accession#: P62722"