LOCUS       V008455                 3793 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008455
VERSION     V008455
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3793)
  AUTHORS   Thole V, Worland B, Snape JW, Vain P.
  TITLE     The pCLEAN dual binary vector system for Agrobacterium-mediated
            plant transformation
  JOURNAL   Plant Physiol. 145 (4), 1211-1219 (2007)
   PUBMED   17932303
REFERENCE   2  (bases 1 to 3793)
  AUTHORS   Thole V, Vain P.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2007) Department of Crop Genetics, John Innes
            Centre, Colney Lane, Norwich NR4 7UH, UK
REFERENCE   3  (bases 1 to 3793)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3793)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant
            Physiol."; date: "2007"; volume: "145"; issue: "4"; pages:
            "1211-1219"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (30-SEP-2007) Department of Crop Genetics, John Innes Centre, Colney
            Lane, Norwich NR4 7UH, UK"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3793
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(61..649)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(823..1635)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      1926..2361
                     /label="pSa ori"
                     /note="origin of replication from bacterial plasmid pSa"
     misc_feature    2508..2532
                     /label="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
     misc_feature    2533..2558
                     /note="26-nt long spacer sequence separating left border
                     No. 1 and No. 2 of the double left border sequence (Thole
                     et al., 2007)"
     misc_feature    2559..2583
                     /note="T-DNA left border No. 2 sequence belonging to a
                     double left border sequence separated by a 26-nt long
                     spacer sequence from left border No. 1 sequence (Thole et
                     al., 2007); left border No. 2 is a 25-nt long consensus
                     sequence of the nopaline vector pTiT37 (Slightom et al.,
                     (1985) EMBO J 4:3069-3077)"
     misc_feature    2584..2635
                     /note="Inner T-DNA region containing 52 nt-long multiple
                     cloning site with NotI, PacI, SfiI, BsiWI, ApaI, XhoI and
                     SalI restriction sites"
     misc_feature    2636..2660
                     /label="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     misc_feature    2661..2684
                     /note="right border overdrive sequence (Peralta et al.
                     (1986) EMBO J 5: 1137-1142)"
     CDS             2894..3694
                     /gene="virG"
                     /label="Regulatory protein VirG"
                     /note="Regulatory protein VirG from Agrobacterium
                     tumefaciens (strain 15955). Accession#: P62722"
ORIGIN
        1 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
       61 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
      121 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      181 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      241 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      301 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      361 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      421 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      481 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
      541 cttcggaaga agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
      601 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
      661 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
      721 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
      781 atcaatctaa agtatatatg tgtaacattg gtctagtgat tagaaaaact catcgagcat
      841 caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg
      901 tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta
      961 tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa
     1021 aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa
     1081 aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa
     1141 atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac
     1201 gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac
     1261 tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc
     1321 tgttttccct gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg
     1381 cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt
     1441 aacaacattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt
     1501 cccatacaat cggtagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata
     1561 cccatataaa tcagcatcca tgttggaatt taatcgcggc cttgagcaag acgtttcccg
     1621 ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt
     1681 tcatgatgat atatttttat cttgtgcaat gtaacatcag agattttgag acacaacgtg
     1741 gctttgttga ataaatcgaa cttttgctga gttgaaggat cagatcacgc atcttcccga
     1801 caacgcagac cgttccgtgg caaagcaaaa gttcaaaatc accaactggt ccacctacaa
     1861 caaagctctc atcaaccgtg gctccctcac tttctggctg gatgatgggg cgattcaggc
     1921 gatccccatc caacagcccg ccgtcgagcg ggctttttta tccccggaag cctgtggata
     1981 gagggtagtt atccacgtga aaccgctaat gccccgcaaa gccttgattc acggggcttt
     2041 ccggcccgct ccaaaaacta tccacgtgaa atcgctaatc agggtacgtg aaatcgctaa
     2101 tcggagtacg tgaaatcgct aataaggtca cgtgaaatcg ctaatcaaaa aggcacgtga
     2161 gaacgctaat agccctttca gatcaacagc ttgcaaacac ccctcgctcc ggcaagtagt
     2221 tacagcaagt agtatgttca attagctttt caattatgaa tatatatatc aattattggt
     2281 cgcccttggc ttgtggacaa tgcgctacgc gcaccggctc cgcccgtgga caaccgcaag
     2341 cggttgccca ccgtcgagcg cctttgccca caacccggcg gccggccgca acagatcgtt
     2401 ttataaattt ttttttttga aaaagaaaaa gcccgaaagg cggcaacctc tcgggcttct
     2461 ggatttccga tccccggaat tagatccgtt taaactacgt aagatcttgg caggatatat
     2521 tgtggtgtaa acgttcctgc ggcggtcgag atggatcttg gcaggatata ttgtggtgta
     2581 aacgttcctg cggccgctta attaaggccg tacgggccct cgagtcgacg ttccttgaca
     2641 ggatatattg gcgggtaaac taagtcgctg tatgtgtttg tttgagatcc tctagggcat
     2701 gcaggctcgc ggcggacgca cgacgccggg gcgagaccat aggcgatctc ctaaatcaat
     2761 agtagctgta acctcgaagc gtttcacttg taacaacgat tgagaatttt tgtcataaaa
     2821 ttgaaatact tggttcgcat ttttgtcatc cgcggtcagc cgcaattctg acgaactgcc
     2881 catttagctg gagatgattg tacatccttc acgtgaaaat ttctcaagcg ctgtgaacaa
     2941 gggttcagat tttagattga aaggtgagcc gttgaaacac gttcttcttg tcgatgacga
     3001 cgtcgctatg cggcatctta ttattgaata ccttacgatc cacgccttca aagtgaccgc
     3061 ggtagccgac agcacccagt tcacaagagt actctcttcc gcgacggtcg atgtcgtggt
     3121 tgttgatcta aatttaggtc gtgaagatgg gctcgagatc gttcgtaatc tggcggcaaa
     3181 gtctgatatt ccaatcataa ttatcagtgg cgaccgcctt gaggagacgg ataaagttgt
     3241 tgcactcgag ctaggagcaa gtgattttat cgctaagccg ttcagtatca gagagtttct
     3301 agcacgcatt cgggttgcct tgcgcgtgcg ccccaacgtt gtccgctcca aagaccgacg
     3361 gtctttttgt tttactgact ggacacttaa tctcaggcaa cgtcgcttga tgtccgaagc
     3421 tggcggtgag gtgaaactta cggcaggtga gttcaatctt ctcctcgcgt ttttagagaa
     3481 accccgcgac gttctatcgc gcgagcaact tctcattgcc agtcgagtac gcgacgagga
     3541 ggtttatgac aggagtatag atgttctcat tttgaggctg cgccgcaaac ttgaggcgga
     3601 tccgtcaagc cctcaactga taaaaacagc aagaggtgcc ggttatttct ttgacgcgga
     3661 cgtgcaggtt tcgcacgggg ggacgatggc agcctgagcc aattgcattt gcctcttaat
     3721 tatctggctc aaagggtgac tgaggagtaa gcgatgtgcc catcacactg cgcatgcaag
     3781 ctgatctgga tct
//
