pCLEAN-G182 vector (V008457)

LOCUS       40924_11011        4347 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Binary vector pCLEAN-G182, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4347)
  AUTHORS   Thole V, Worland B, Snape JW, Vain P.
  TITLE     The pCLEAN dual binary vector system for Agrobacterium-mediated 
            plant transformation
  JOURNAL   Plant Physiol. 145 (4), 1211-1219 (2007)
  PUBMED    17932303
REFERENCE   2  (bases 1 to 4347)
  AUTHORS   Thole V, Vain P.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-OCT-2007) Department of Crop Genetics, John Innes 
            Centre, Colney Lane, Norwich NR4 7UH, UK
REFERENCE   3  (bases 1 to 4347)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4347)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2007"; volume: "145"; issue: "4"; pages: 
            "1211-1219"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (01-OCT-2007) Department of Crop Genetics, John Innes Centre, Colney
            Lane, Norwich NR4 7UH, UK"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4347
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(61..649)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(823..1635)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      1926..2361
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     regulatory      complement(2536..2832)
                     /label=soybean-derived poly(A)signal
                     /note="soybean-derived poly(A)signal"
                     /regulatory_class="terminator"
     CDS             complement(2868..3578)
                     /label=mgfp5
                     /note="GFP with folding enhancement mutations"
     promoter        complement(3677..4021)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    4137..4161
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     misc_feature    4162..4187
                     /note="26-nt long spacer sequence separating left border
                     No. 1 and No. 2 of the double left border sequence (Thole 
                     et al., 2007)"
     misc_feature    4188..4212
                     /note="T-DNA left border No. 2 sequence belonging to a
                     double left border sequence separated by a 26-nt long 
                     spacer sequence from left border No. 1 sequence (Thole et 
                     al., 2007); left border No. 2 is a 25-nt long consensus 
                     sequence of the nopaline vector pTiT37 (Slightom et al., 
                     (1985) EMBO J 4:3069-3077)"
     misc_feature    4213..4264
                     /note="Inner T-DNA region containing 52 nt-long multiple
                     cloning site with NotI, PacI, SfiI, BsiWI, ApaI, XhoI and 
                     SalI restriction sites"
     misc_feature    4265..4289
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     misc_feature    4290..4313
                     /note="right border overdrive sequence (Peralta et al.
                     (1986) EMBO J 5: 1137-1142)"