LOCUS       40924_11011        4347 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Binary vector pCLEAN-G182, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4347)
  AUTHORS   Thole V, Worland B, Snape JW, Vain P.
  TITLE     The pCLEAN dual binary vector system for Agrobacterium-mediated 
            plant transformation
  JOURNAL   Plant Physiol. 145 (4), 1211-1219 (2007)
  PUBMED    17932303
REFERENCE   2  (bases 1 to 4347)
  AUTHORS   Thole V, Vain P.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-OCT-2007) Department of Crop Genetics, John Innes 
            Centre, Colney Lane, Norwich NR4 7UH, UK
REFERENCE   3  (bases 1 to 4347)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4347)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2007"; volume: "145"; issue: "4"; pages: 
            "1211-1219"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (01-OCT-2007) Department of Crop Genetics, John Innes Centre, Colney
            Lane, Norwich NR4 7UH, UK"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4347
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(61..649)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(823..1635)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      1926..2361
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     regulatory      complement(2536..2832)
                     /label=soybean-derived poly(A)signal
                     /note="soybean-derived poly(A)signal"
                     /regulatory_class="terminator"
     CDS             complement(2868..3578)
                     /label=mgfp5
                     /note="GFP with folding enhancement mutations"
     promoter        complement(3677..4021)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    4137..4161
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     misc_feature    4162..4187
                     /note="26-nt long spacer sequence separating left border
                     No. 1 and No. 2 of the double left border sequence (Thole 
                     et al., 2007)"
     misc_feature    4188..4212
                     /note="T-DNA left border No. 2 sequence belonging to a
                     double left border sequence separated by a 26-nt long 
                     spacer sequence from left border No. 1 sequence (Thole et 
                     al., 2007); left border No. 2 is a 25-nt long consensus 
                     sequence of the nopaline vector pTiT37 (Slightom et al., 
                     (1985) EMBO J 4:3069-3077)"
     misc_feature    4213..4264
                     /note="Inner T-DNA region containing 52 nt-long multiple
                     cloning site with NotI, PacI, SfiI, BsiWI, ApaI, XhoI and 
                     SalI restriction sites"
     misc_feature    4265..4289
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     misc_feature    4290..4313
                     /note="right border overdrive sequence (Peralta et al.
                     (1986) EMBO J 5: 1137-1142)"
ORIGIN
        1 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
       61 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
      121 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      181 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      241 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      301 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      361 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      421 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      481 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
      541 cttcggaaga agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
      601 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
      661 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
      721 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
      781 atcaatctaa agtatatatg tgtaacattg gtctagtgat tagaaaaact catcgagcat
      841 caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg
      901 tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta
      961 tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa
     1021 aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa
     1081 aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa
     1141 atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac
     1201 gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac
     1261 tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc
     1321 tgttttccct gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg
     1381 cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt
     1441 aacaacattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt
     1501 cccatacaat cggtagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata
     1561 cccatataaa tcagcatcca tgttggaatt taatcgcggc cttgagcaag acgtttcccg
     1621 ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt
     1681 tcatgatgat atatttttat cttgtgcaat gtaacatcag agattttgag acacaacgtg
     1741 gctttgttga ataaatcgaa cttttgctga gttgaaggat cagatcacgc atcttcccga
     1801 caacgcagac cgttccgtgg caaagcaaaa gttcaaaatc accaactggt ccacctacaa
     1861 caaagctctc atcaaccgtg gctccctcac tttctggctg gatgatgggg cgattcaggc
     1921 gatccccatc caacagcccg ccgtcgagcg ggctttttta tccccggaag cctgtggata
     1981 gagggtagtt atccacgtga aaccgctaat gccccgcaaa gccttgattc acggggcttt
     2041 ccggcccgct ccaaaaacta tccacgtgaa atcgctaatc agggtacgtg aaatcgctaa
     2101 tcggagtacg tgaaatcgct aataaggtca cgtgaaatcg ctaatcaaaa aggcacgtga
     2161 gaacgctaat agccctttca gatcaacagc ttgcaaacac ccctcgctcc ggcaagtagt
     2221 tacagcaagt agtatgttca attagctttt caattatgaa tatatatatc aattattggt
     2281 cgcccttggc ttgtggacaa tgcgctacgc gcaccggctc cgcccgtgga caaccgcaag
     2341 cggttgccca ccgtcgagcg cctttgccca caacccggcg gccggccgca acagatcgtt
     2401 ttataaattt ttttttttga aaaagaaaaa gcccgaaagg cggcaacctc tcgggcttct
     2461 ggatttccga tccccggaat tagatccgtt taaacttcga cggtatcgag cttgatatcg
     2521 aattgatccg aattcctttg ttaaccatca tttagtgaca caattaactt aaatttagaa
     2581 gagactaatt tcaacccaca agatggaaat gcaaggacaa tgggttcttg aagttatttg
     2641 gaaacaaagc aacacaacac aatagcttat tcattagttc tcatccatta atttccattg
     2701 aagtccgaaa acagaaacca aaaatacagg atgatgaaat ctcataaagc aaaaacaaat
     2761 gggaatgtag tttaagtaca acaaacaaag tggggtggct acagatgctt tgcaaatttt
     2821 tcaatgactt acggatcaat tcctgcagcc ccttaaagct catcatgttt gtatagttca
     2881 tccatgccat gtgtaatccc agcagctgtt acaaactcaa gaaggaccat gtggtctctc
     2941 ttttcgttgg gatctttcga aagggcagat tgtgtggaca ggtaatggtt gtctggtaaa
     3001 aggacagggc catcgccaat tggagtattt tgttgataat gatcagcgag ttgcacgccg
     3061 ccgtcttcga tgttgtggcg ggtcttgaag ttggctttga tgccgttctt ttgcttgtcg
     3121 gccatgatgt atacgttgtg ggagttgtag ttgtattcca acttgtggcc gaggatgttt
     3181 ccgtcctcct tgaaatcgat tcccttaagc tcgatcctgt tgacgagggt gtctccctca
     3241 aacttgactt cagcacgtgt cttgtagttc ccgtcgtcct tgaagaagat ggtcctctcc
     3301 tgcacgtatc cctcaggcat ggcgctcttg aagaagtcgt gccgcttcat atgatctggg
     3361 tatcttgaaa agcattgaac accataagag aaagtagtga caagtgttgg ccatggaaca
     3421 ggtagttttc cagtagtgca aataaattta agggtaagtt ttccgtatgt tgcatcacct
     3481 tcaccctctc cactgacaga aaatttgtac ccattaacat caccatctaa ttcaacaaga
     3541 attgggacaa ctccagtgaa aagttcttct cctttactga attcggccga ggataatgat
     3601 aggagaagtg aaaagatgag aaagagaaaa agattagtct tcattgttat atctccttgg
     3661 atcctctaga gtcgaggtcc tctccaaatg aaatgaactt ccttatatag aggaagggtc
     3721 ttgcgaagga tagtgggatt gtgcgtcatc ccttacgtca gtggagatat cacatcaatc
     3781 cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc acgatgttcc tcgtgggtgg
     3841 gggtccatct ttgggaccac tgtcggtaga ggcatcttga acgatagcct ttcctttatc
     3901 gcaatgatgg catttgtaga agccatcttc cttttctact gtcctttcga tgaagtgaca
     3961 gatagctggg caatggaatc cgaggaggtt tcccgatatt accctttgtt gaaaagtctc
     4021 aatagccctc tggtcttctg agactgtatc tttgatattc ttggagtaga cgagagtgtc
     4081 gtgctccacc atgttgacct gcaggcatgc aagctggggg atccactaga agatcttggc
     4141 aggatatatt gtggtgtaaa cgttcctgcg gcggtcgaga tggatcttgg caggatatat
     4201 tgtggtgtaa acgttcctgc ggccgcttaa ttaaggccgt acgggccctc gagtcgacgt
     4261 tccttgacag gatatattgg cgggtaaact aagtcgctgt atgtgtttgt ttgagatcct
     4321 ctagggcatg caagctgatc tggatct
//
