pCK300 vector (V008500)

LOCUS       40924_10796        3822 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Promoter vector pCK300, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3822)
  AUTHORS   Kelly CL, Liu Z, Yoshihara A, Jenkinson SF, Wormald MR, Otero JM, 
            Estevez AM, Kato A, Marqvorsen MH, Fleet GW, Estevez RJ, Izumori K, 
            Heap JT.
  TITLE     Synthetic chemical inducers and genetic decoupling enable orthogonal
            control of the rhaBAD promoter
  JOURNAL   ACS Synth Biol (2016) In press
  PUBMED    27247275
REFERENCE   2  (bases 1 to 3822)
  AUTHORS   Kelly CL, Liu Z, Yoshihara A, Jenkinson SF, Wormald MR, Otero J, 
            Estevez A, Kato A, Marqvorsen MHS., Fleet GWJ., Estevez RJ, Izumori 
            K, Heap JT.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2016) Centre for Synthetic Biology and Innovation,
            Department of Life Sciences, Imperial College London, South 
            Kensington Campus, London SW7 2AZ, United Kingdom
REFERENCE   3  (bases 1 to 3822)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3822)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol (2016) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (18-JAN-2016) Centre for Synthetic Biology and Innovation, 
            Department of Life Sciences, Imperial College London, South 
            Kensington Campus, London SW7 2AZ, United Kingdom"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3822
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      17..605
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     875..891
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      complement(908..1021)
                     /label=rpn txn term terminator
                     /note="rpn txn term terminator"
                     /regulatory_class="terminator"
     regulatory      1028..1328
                     /label=bla txn term terminator
                     /note="bla txn term terminator"
                     /regulatory_class="terminator"
     regulatory      1375..1549
                     /label=rrnB1 B2 T1 term terminator
                     /note="rrnB1 B2 T1 term terminator"
                     /regulatory_class="terminator"
     promoter        1636..1727
                     /gene="bla"
                     /label=AmpR promoter
     regulatory      1658..1686
                     /label=Pamp promoter
                     /note="Pamp promoter"
                     /regulatory_class="promoter"
     CDS             1728..2585
                     /label=AmpR
                     /note="beta-lactamase"
     CDS             2671..3750
                     /label=lacI
                     /note="lac repressor"