LOCUS       40924_10796        3822 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Promoter vector pCK300, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3822)
  AUTHORS   Kelly CL, Liu Z, Yoshihara A, Jenkinson SF, Wormald MR, Otero JM, 
            Estevez AM, Kato A, Marqvorsen MH, Fleet GW, Estevez RJ, Izumori K, 
            Heap JT.
  TITLE     Synthetic chemical inducers and genetic decoupling enable orthogonal
            control of the rhaBAD promoter
  JOURNAL   ACS Synth Biol (2016) In press
  PUBMED    27247275
REFERENCE   2  (bases 1 to 3822)
  AUTHORS   Kelly CL, Liu Z, Yoshihara A, Jenkinson SF, Wormald MR, Otero J, 
            Estevez A, Kato A, Marqvorsen MHS., Fleet GWJ., Estevez RJ, Izumori 
            K, Heap JT.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2016) Centre for Synthetic Biology and Innovation,
            Department of Life Sciences, Imperial College London, South 
            Kensington Campus, London SW7 2AZ, United Kingdom
REFERENCE   3  (bases 1 to 3822)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3822)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol (2016) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (18-JAN-2016) Centre for Synthetic Biology and Innovation, 
            Department of Life Sciences, Imperial College London, South 
            Kensington Campus, London SW7 2AZ, United Kingdom"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3822
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      17..605
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     875..891
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      complement(908..1021)
                     /label=rpn txn term terminator
                     /note="rpn txn term terminator"
                     /regulatory_class="terminator"
     regulatory      1028..1328
                     /label=bla txn term terminator
                     /note="bla txn term terminator"
                     /regulatory_class="terminator"
     regulatory      1375..1549
                     /label=rrnB1 B2 T1 term terminator
                     /note="rrnB1 B2 T1 term terminator"
                     /regulatory_class="terminator"
     promoter        1636..1727
                     /gene="bla"
                     /label=AmpR promoter
     regulatory      1658..1686
                     /label=Pamp promoter
                     /note="Pamp promoter"
                     /regulatory_class="promoter"
     CDS             1728..2585
                     /label=AmpR
                     /note="beta-lactamase"
     CDS             2671..3750
                     /label=lacI
                     /note="lac repressor"
ORIGIN
        1 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
       61 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
      121 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
      181 agttagccca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
      241 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
      301 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
      361 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
      421 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
      481 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
      541 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
      601 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
      661 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
      721 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
      781 cggaaggcga gagtagggaa ctgccaggca tcaaactaag cagaaggccc ctgacggatg
      841 gcctttttgc gtttctacaa actctttctg tgttgtaaaa cgacggccag tcttaagctc
      901 gggccccctg ggcggttctg ataacgagta atcgttaatc cgcaaataac gtaaaaaccc
      961 gcttcggcgg gtttttttat ggggggagtt tagggaaaga gcatttgtca gaatatttaa
     1021 gggcgcctgt cactttgctt gatatatgag aattatttaa ccttataaat gagaaaaaag
     1081 caacgcactt taaataagat acgttgcttt ttcgattgat gaacacctat aattaaacta
     1141 ttcatctatt atttatgatt ttttgtatat acaatatttc tagtttgtta aagagaatta
     1201 agaaaataaa tctcgaaaat aataaaggga aaatcagttt ttgatatcaa aattatacat
     1261 gtcaacgata atacaaaata taatacaaac tataagatgt tatcagtatt tattatgcat
     1321 ttagaataaa ttttgtgtcg ccctttaact cgagccccaa gggcgacacc ccataattag
     1381 cccgggcgaa aggcccagtc tttcgactga gcctttcgtt ttatttgatg cctggcagtt
     1441 ccctactctc gcatggggag tccccacact accatcggcg ctacggcgtt tcacttctga
     1501 gttcggcatg gggtcaggtg ggaccaccgc gctactgccg ccaggcaaac aaggggtgtt
     1561 atgagccata ttcaggtata aatgggctcg cgataatgtt cagaattggt taattggttg
     1621 taacactgac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     1681 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagaatatg agtattcaac
     1741 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     1801 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     1861 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     1921 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     1981 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     2041 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     2101 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     2161 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     2221 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcgatgg
     2281 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     2341 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     2401 ctggctggtt tattgctgat aaatccggag ccggtgagcg tggttctcgc ggtatcatcg
     2461 cagcgctggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     2521 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     2581 attggtaagc ggcgcgccat cgaatggcgc aaaacctttc gcggtatggc atgatagcgc
     2641 ccggaagaga gtcaattcag ggtggtgaat atgaaaccag taacgttata cgatgtcgca
     2701 gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt
     2761 tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta cattcccaac
     2821 cgcgtggcac aacaactggc gggcaaacag tcgttgctga ttggcgttgc cacctccagt
     2881 ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg
     2941 ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg
     3001 gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca ttaactatcc gctggatgac
     3061 caggatgcca ttgctgtgga agctgcctgc actaatgttc cggcgttatt tcttgatgtc
     3121 tctgaccaga cacccatcaa cagtattatt ttctcccatg aggacggtac gcgactgggc
     3181 gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg cccattaagt
     3241 tctgtctcgg cgcgtctgcg tctggctggc tggcataaat atctcactcg caatcaaatt
     3301 cagccgatag cggaacggga aggcgactgg agtgccatgt ccggttttca acaaaccatg
     3361 caaatgctga atgagggcat cgttcccact gcgatgctgg ttgccaacga tcagatggcg
     3421 ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga tatctcggta
     3481 gtgggatacg acgataccga agatagctca tgttatatcc cgccgttaac caccatcaaa
     3541 caggattttc gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc
     3601 caggcggtga agggcaatca gctgttgcca gtctcactgg tgaaaagaaa aaccaccctg
     3661 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     3721 cgacaggttt cccgactgga aagcgggcag tgactcatga ccaaaatccc ttaacgtgag
     3781 ttacgcgcgc gtcgttccac tgagcgtcag accccgtaga aa
//
