pCITCAM101 vector (V008509)

LOCUS       V008509                 9297 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008509
VERSION     V008509
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9297)
  AUTHORS   Dutt M, Lee DH, Grosser JW.
  TITLE     Bifunctional selection-reporter systems for genetic transformation
            of citrus: mannose and kanamycin based systems
  JOURNAL   In Vitro Cell. Dev. Biol., Plant 46, 467-476 (2010)
REFERENCE   2  (bases 1 to 9297)
  AUTHORS   Dutt M, Lee DH, Grosser JW.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2011) Citrus Research and Education Center,
            University of Florida, 700 Experiment Station Road, Lake Alfred, FL
            33850, USA
REFERENCE   3  (bases 1 to 9297)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9297)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "In Vitro
            Cell. Dev. Biol., Plant 46, 467-476 (2010)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (04-OCT-2011) Citrus Research and Education Center, University of
            Florida, 700 Experiment Station Road, Lake Alfred, FL 33850, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9297
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        276..619
                     /label="CaMV 35S promoter"
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    630..685
                     /label="TMV Omega"
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             694..1866
                     /gene="manA"
                     /label="Mannose-6-phosphate isomerase"
                     /note="Mannose-6-phosphate isomerase from Escherichia coli
                     (strain K12). Accession#: P00946"
     CDS             1873..1890
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             1891..2604
                     /label="EGFP"
                     /note="enhanced GFP"
     terminator      2638..2890
                     /label="NOS terminator"
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    2912..2936
                     /label="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4236..4862
                     /label="pVS1 StaA"
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             5299..6363
                     /label="pVS1 RepA"
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      6432..6626
                     /label="pVS1 oriV"
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    6970..7110
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(7296..7884)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7974..8765)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     misc_feature    9190..9214
                     /label="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"