LOCUS       V008509                 9297 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008509
VERSION     V008509
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9297)
  AUTHORS   Dutt M, Lee DH, Grosser JW.
  TITLE     Bifunctional selection-reporter systems for genetic transformation
            of citrus: mannose and kanamycin based systems
  JOURNAL   In Vitro Cell. Dev. Biol., Plant 46, 467-476 (2010)
REFERENCE   2  (bases 1 to 9297)
  AUTHORS   Dutt M, Lee DH, Grosser JW.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2011) Citrus Research and Education Center,
            University of Florida, 700 Experiment Station Road, Lake Alfred, FL
            33850, USA
REFERENCE   3  (bases 1 to 9297)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9297)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "In Vitro
            Cell. Dev. Biol., Plant 46, 467-476 (2010)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (04-OCT-2011) Citrus Research and Education Center, University of
            Florida, 700 Experiment Station Road, Lake Alfred, FL 33850, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9297
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        276..619
                     /label="CaMV 35S promoter"
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    630..685
                     /label="TMV Omega"
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             694..1866
                     /gene="manA"
                     /label="Mannose-6-phosphate isomerase"
                     /note="Mannose-6-phosphate isomerase from Escherichia coli
                     (strain K12). Accession#: P00946"
     CDS             1873..1890
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             1891..2604
                     /label="EGFP"
                     /note="enhanced GFP"
     terminator      2638..2890
                     /label="NOS terminator"
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    2912..2936
                     /label="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4236..4862
                     /label="pVS1 StaA"
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             5299..6363
                     /label="pVS1 RepA"
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      6432..6626
                     /label="pVS1 oriV"
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    6970..7110
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(7296..7884)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7974..8765)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     misc_feature    9190..9214
                     /label="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 agcttgcctc cgattgagac ttttcaacaa agggtaatat ccggaaacct cctcggattc
       61 cattgcccag ctatctgtca ctttattgtg aagatagtgg aaaaggaagg tggctcctac
      121 aaatgccatc attgcgataa aggaaaggcc atcgttgaag atgcctctgc cgacagtggt
      181 cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaagacgt tccaaccacg
      241 tcttcaaagc aagtggattg atgtgatggt ccgattgaga cttttcaaca aagggtaata
      301 tccggaaacc tcctcggatt ccattgccca gctatctgtc actttattgt gaagatagtg
      361 gaaaaggaag gtggctccta caaatgccat cattgcgata aaggaaaggc catcgttgaa
      421 gatgcctctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa
      481 aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgatat ctccactgac
      541 gtaagggatg acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt
      601 tcatttcatt tggagaggat atttttacaa caattaccaa caacaacaaa caacaaacaa
      661 cattacaatt actatttaca attacaggat ccgatgcaaa aactcattaa ctcagtgcaa
      721 aactatgcct ggggcagcaa aacggcgttg actgaacttt atggtatgga aaatccgtcc
      781 agccagccga tggccgagct gtggatgggc gcacatccga aaagcagttc acgagtgcag
      841 aatgccgccg gagatatcgt ttcactgcgt gatgtgattg agagtgataa atcgactctg
      901 ctcggagagg ccgttgccaa acgctttggc gaactgcctt tcctgttcaa agtattatgc
      961 gcagcacagc cactctccat tcaggttcat ccaaacaaac acaattctga aatcggtttt
     1021 gccaaagaaa atgccgcagg tatcccgatg gatgccgccg agcgtaacta taaagatcct
     1081 aaccacaagc cggagctggt ttttgcgctg acgcctttcc ttgcgatgaa cgcgtttcgt
     1141 gaattttccg agattgtctc cctactccag ccggtcgcag gtgcacatcc ggcgattgct
     1201 cactttttac aacagcctga tgccgaacgt ttaagcgaac tgttcgccag cctgttgaat
     1261 atgcagggtg aagaaaaatc ccgcgcgctg gcgattttaa aatcggccct cgatagccag
     1321 cagggtgaac cgtggcaaac gattcgttta atttctgaat tttacccgga agacagcggt
     1381 ctgttctccc cgctattgct gaatgtggtg aaattgaacc ctggcgaagc gatgttcctg
     1441 ttcgctgaaa caccgcacgc ttacctgcaa ggcgtggcgc tggaagtgat ggcaaactcc
     1501 gataacgtgc tgcgtgcggg tctgacgcct aaatacattg atattccgga actggttgcc
     1561 aatgtgaaat tcgaagccaa accggctaac cagttgttga cccagccggt gaaacaaggt
     1621 gcagaactgg acttcccgat tccagtggat gattttgcct tctcgctgca tgaccttagt
     1681 gataaagaaa ccaccattag ccagcagagt gccgccattt tgttctgcgt cgaaggcgat
     1741 gcaacgttgt ggaaaggttc tcagcagtta cagcttaaac cgggtgaatc agcgtttatt
     1801 gccgccaacg aatcaccggt gactgtcaaa ggccacggcc gtttagcgcg tgtttacaac
     1861 aagctgcagg gccatcatca tcaccatcat gtgagcaagg gcgaggagct gttcaccggg
     1921 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
     1981 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
     2041 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc
     2101 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
     2161 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
     2221 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     2281 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
     2341 tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac
     2401 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
     2461 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac
     2521 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
     2581 ctcggcatgg acgagctgta caagtgataa agcggccgcg actctagaca ggcctacgat
     2641 cgttcaaaca tttggcaata aagtttctta agattgaatc ctgttgccgg tcttgcgatg
     2701 attatcatat aatttctgtt gaattacgtt aagcatgtaa taattaacat gtaatgcatg
     2761 acgttattta tgagatgggt ttttatgatt agagtcccgc aattatacat ttaatacgcg
     2821 atagaaaaca aaatatagcg cgcaaactag gataaattat cgcgcgcggt gtcatctatg
     2881 ttactagatc gggaattaaa ctatcagtgt ttgacaggat atattggcgg gtaaacctaa
     2941 gagaaaagag cgtttattag aataacggat atttaaaagg gcgtgaaaag gtttatccgt
     3001 tcgtccattt gtatgtgcat gccaaccaca gggttcccct cgggatcaaa gtactttgat
     3061 ccaacccctc cgctgctata gtgcagtcgg cttctgacgt tcagtgcagc cgtcttctga
     3121 aaacgacatg tcgcacaagt cctaagttac gcgacaggct gccgccctgc ccttttcctg
     3181 gcgttttctt gtcgcgtgtt ttagtcgcat aaagtagaat acttgcgact agaaccggag
     3241 acattacgcc atgaacaaga gcgccgccgc tggcctgctg ggctatgccc gcgtcagcac
     3301 cgacgaccag gacttgacca accaacgggc cgaactgcac gcggccggct gcaccaagct
     3361 gttttccgag aagatcaccg gcaccaggcg cgaccgcccg gagctggcca ggatgcttga
     3421 ccacctacgc cctggcgacg ttgtgacagt gaccaggcta gaccgcctgg cccgcagcac
     3481 ccgcgaccta ctggacattg ccgagcgcat ccaggaggcc ggcgcgggcc tgcgtagcct
     3541 ggcagagccg tgggccgaca ccaccacgcc ggccggccgc atggtgttga ccgtgttcgc
     3601 cggcattgcc gagttcgagc gttccctaat catcgaccgc acccggagcg ggcgcgaggc
     3661 cgccaaggcc cgaggcgtga agtttggccc ccgccctacc ctcaccccgg cacagatcgc
     3721 gcacgcccgc gagctgatcg accaggaagg ccgcaccgtg aaagaggcgg ctgcactgct
     3781 tggcgtgcat cgctcgaccc tgtaccgcgc acttgagcgc agcgaggaag tgacgcccac
     3841 cgaggccagg cggcgcggtg ccttccgtga ggacgcattg accgaggccg acgccctggc
     3901 ggccgccgag aatgaacgcc aagaggaaca agcatgaaac cgcaccagga cggccaggac
     3961 gaaccgtttt tcattaccga agagatcgag gcggagatga tcgcggccgg gtacgtgttc
     4021 gagccgcccg cgcacgtctc aaccgtgcgg ctgcatgaaa tcctggccgg tttgtctgat
     4081 gccaagctgg cggcctggcc ggccagcttg gccgctgaag aaaccgagcg ccgccgtcta
     4141 aaaaggtgat gtgtatttga gtaaaacagc ttgcgtcatg cggtcgctgc gtatatgatg
     4201 cgatgagtaa ataaacaaat acgcaagggg aacgcatgaa ggttatcgct gtacttaacc
     4261 agaaaggcgg gtcaggcaag acgaccatcg caacccatct agcccgcgcc ctgcaactcg
     4321 ccggggccga tgttctgtta gtcgattccg atccccaggg cagtgcccgc gattgggcgg
     4381 ccgtgcggga agatcaaccg ctaaccgttg tcggcatcga ccgcccgacg attgaccgcg
     4441 acgtgaaggc catcggccgg cgcgacttcg tagtgatcga cggagcgccc caggcggcgg
     4501 acttggctgt gtccgcgatc aaggcagccg acttcgtgct gattccggtg cagccaagcc
     4561 cttacgacat atgggccacc gccgacctgg tggagctggt taagcagcgc attgaggtca
     4621 cggatggaag gctacaagcg gcctttgtcg tgtcgcgggc gatcaaaggc acgcgcatcg
     4681 gcggtgaggt tgccgaggcg ctggccgggt acgagctgcc cattcttgag tcccgtatca
     4741 cgcagcgcgt gagctaccca ggcactgccg ccgccggcac aaccgttctt gaatcagaac
     4801 ccgagggcga cgctgcccgc gaggtccagg cgctggccgc tgaaattaaa tcaaaactca
     4861 tttgagttaa tgaggtaaag agaaaatgag caaaagcaca aacacgctaa gtgccggccg
     4921 tccgagcgca cgcagcagca aggctgcaac gttggccagc ctggcagaca cgccagccat
     4981 gaagcgggtc aactttcagt tgccggcgga ggatcacacc aagctgaaga tgtacgcggt
     5041 acgccaaggc aagaccatta ccgagctgct atctgaatac atcgcgcagc taccagagta
     5101 aatgagcaaa tgaataaatg agtagatgaa ttttagcggc taaaggaggc ggcatggaaa
     5161 atcaagaaca accaggcacc gacgccgtgg aatgccccat gtgtggagga acgggcggtt
     5221 ggccaggcgt aagcggctgg gttgtctgcc ggccctgcaa tggcactgga acccccaagc
     5281 ccgaggaatc ggcgtgacgg tcgcaaacca tccggcccgg tacaaatcgg cgcggcgctg
     5341 ggtgatgacc tggtggagaa gttgaaggcc gcgcaggccg cccagcggca acgcatcgag
     5401 gcagaagcac gccccggtga atcgtggcaa gcggccgctg atcgaatccg caaagaatcc
     5461 cggcaaccgc cggcagccgg tgcgccgtcg attaggaagc cgcccaaggg cgacgagcaa
     5521 ccagattttt tcgttccgat gctctatgac gtgggcaccc gcgatagtcg cagcatcatg
     5581 gacgtggccg ttttccgtct gtcgaagcgt gaccgacgag ctggcgaggt gatccgctac
     5641 gagcttccag acgggcacgt agaggtttcc gcagggccgg ccggcatggc cagtgtgtgg
     5701 gattacgacc tggtactgat ggcggtttcc catctaaccg aatccatgaa ccgataccgg
     5761 gaagggaagg gagacaagcc cggccgcgtg ttccgtccac acgttgcgga cgtactcaag
     5821 ttctgccggc gagccgatgg cggaaagcag aaagacgacc tggtagaaac ctgcattcgg
     5881 ttaaacacca cgcacgttgc catgcagcgt acgaagaagg ccaagaacgg ccgcctggtg
     5941 acggtatccg agggtgaagc cttgattagc cgctacaaga tcgtaaagag cgaaaccggg
     6001 cggccggagt acatcgagat cgagctagct gattggatgt accgcgagat cacagaaggc
     6061 aagaacccgg acgtgctgac ggttcacccc gattactttt tgatcgatcc cggcatcggc
     6121 cgttttctct accgcctggc acgccgcgcc gcaggcaagg cagaagccag atggttgttc
     6181 aagacgatct acgaacgcag tggcagcgcc ggagagttca agaagttctg tttcaccgtg
     6241 cgcaagctga tcgggtcaaa tgacctgccg gagtacgatt tgaaggagga ggcggggcag
     6301 gctggcccga tcctagtcat gcgctaccgc aacctgatcg agggcgaagc atccgccggt
     6361 tcctaatgta cggagcagat gctagggcaa attgccctag caggggaaaa aggtcgaaaa
     6421 ggtctctttc ctgtggatag cacgtacatt gggaacccaa agccgtacat tgggaaccgg
     6481 aacccgtaca ttgggaaccc aaagccgtac attgggaacc ggtcacacat gtaagtgact
     6541 gatataaaag agaaaaaagg cgatttttcc gcctaaaact ctttaaaact tattaaaact
     6601 cttaaaaccc gcctggcctg tgcataactg tctggccagc gcacagccga agagctgcaa
     6661 aaagcgccta cccttcggtc gctgcgctcc ctacgccccg ccgcttcgcg tcggcctatc
     6721 gcggccgctg gccgctcaaa aatggctggc ctacggccag gcaatctacc agggcgcgga
     6781 caagccgcgc cgtcgccact cgaccgccgg cgcccacatc aaggcaccct gcctcgcgcg
     6841 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg
     6901 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg
     6961 gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata ctggcttaac
     7021 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac
     7081 agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg
     7141 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     7201 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     7261 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     7321 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     7381 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     7441 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     7501 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     7561 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     7621 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     7681 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
     7741 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     7801 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     7861 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     7921 cagtggaacg aaaactcacg ttaagggatt ttggtcatgc attctaggta ctaaaacaat
     7981 tcatccagta aaatataata ttttattttc tcccaatcag gcttgatccc cagtaagtca
     8041 aaaaatagct cgacatactg ttcttccccg atatcctccc tgatcgaccg gacgcagaag
     8101 gcaatgtcat accacttgtc cgccctgccg cttctcccaa gatcaataaa gccacttact
     8161 ttgccatctt tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa gacaagttcc
     8221 tcttcgggct tttccgtctt taaaaaatca tacagctcgc gcggatcttt aaatggagtg
     8281 tcttcttccc agttttcgca atccacatcg gccagatcgt tattcagtaa gtaatccaat
     8341 tcggctaagc ggctgtctaa gctattcgta tagggacaat ccgatatgtc gatggagtga
     8401 aagagcctga tgcactccgc atacagctcg ataatctttt cagggctttg ttcatcttca
     8461 tactcttccg agcaaaggac gccatcggcc tcactcatga gcagattgct ccagccatca
     8521 tgccgttcaa agtgcaggac ctttggaaca ggcagctttc cttccagcca tagcatcatg
     8581 tccttttccc gttccacatc ataggtggtc cctttatacc ggctgtccgt catttttaaa
     8641 tataggtttt cattttctcc caccagctta tataccttag caggagacat tccttccgta
     8701 tcttttacgc agcggtattt ttcgatcagt tttttcaatt ccggtgatat tctcatttta
     8761 gccatttatt atttccttcc tcttttctac agtatttaaa gataccccaa gaagctaatt
     8821 ataacaagac gaactccaat tcactgttcc ttgcattcta aaaccttaaa taccagaaaa
     8881 cagctttttc aaagttgttt tcaaagttgg cgtataacat agtatcgacg gagccgattt
     8941 tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc
     9001 tccgcgagat catccgtgtt tcaaacccgg cagcttagtt gccgttcttc cgaatagcat
     9061 cggtaacatg agcaaagtct gccgccttac aacggctctc ccgctgacgc cgtcccggac
     9121 tgatgggctg cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg
     9181 gctggctggt ggcaggatat attgtggtgt aaacaaattg acgcttagac aacttaataa
     9241 cacattgcgg acgtttttaa tgtactgaat taacgccgaa ttaattccta gggccca
//
