pCHIK-37997-5GFP vector (V008524)

LOCUS       V008524                14713 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008524
VERSION     V008524
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 14713)
  AUTHORS   Tsetsarkin KA, Vanlandingham DL, Higgs S.
  TITLE     GFP-expressing full length clone of Chikungunya virus strain 37997
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14713)
  AUTHORS   Tsetsarkin KA, Vanlandingham DL, Higgs S.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-OCT-2007) Pathology, University of Texas Medical
            Branch, University Blvd, Galveston, TX 77555-0609, USA
REFERENCE   3  (bases 1 to 14713)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 14713)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (17-OCT-2007) Pathology, University of Texas Medical Branch,
            University Blvd, Galveston, TX 77555-0609, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..14713
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     5'UTR           1..77
     CDS             78..7502
                     /codon_start=1
                     /transl_except=(pos: 5646 .. 5648,  aa: OTHER)
                     /product="nonstructural polyprotein"
                     /label="nonstructural polyprotein"
                     /note="encodes nonstructural proteins 1 through 4"
                     /protein_id="ABX40012.1"
                     /translation="MDPVYVDIDADSAFLKALQRAYPMFEVEPRQVTPNDHANARAFSH
                     LAIKLIEQEIDPDSTILDIGSAPARRMMSDRKYHCVCPMRSAEDPERLANYARKLASAA
                     GKVLDRNISEKIGDLQAVMAVPDAETPTFCLHTDVSCRQRADVAIYQDVYAVHAPTSLY
                     HQAIKGVRVAYWIGFDTTPFMYNAMAGAYPSYSTNWADEQVLKAKNIGLCSTDLTEGRR
                     GKLSIMRGKKMKPCDRVLFSVGSTLYPESRKLLKSWHLPSVFHLKGKLSFTCRCDTVVS
                     CEGYVVKRITISPGLYGKTTGYAVTHHADGFLMCKTTDTVDGERVSFSVCTYVPATICD
                     QMTGILATEVTPEDAQKLLVGLNQRIVVNGRTQRNTNTMKNYLLPVVAQALSKWAKECR
                     KDMEDEKLLGIRERTLTCCCLWAFKKQKTHTVYKRPDTQSIQKVPAEFDSFVVPSLWSS
                     GLSIPLRTRIKWLLSKVPKTDLIPYSGDAKEARDAEKEAEEEREAELTREALPPLQAAQ
                     DDVQVEIDVEQLEDRAGAGIIETPRGAIKVTAQPTDHVVGEYLVLSPQTVLRSQKLSLI
                     HALAEQVKTCTHSGRAGRYAVEAYDGRILVPSGYAISPEDFQSLSESATMVYNEREFVN
                     RKLHHIALHGPALNTDEESYELVRAERTEHEYVYDVDQRRCCKKEEAAGLVLVGDLTNP
                     PYHEFAYEGLRIRPACPYKTAVIGVFGVPGSGKSAIIKNLVTRQDLVTSGKKENCQEIS
                     TDVMRQRNLEISARTVDSLLLNGCNRPVDVLYVDEAFACHSGTLLALIALVRPRQKVVL
                     CGDPKQCGFFNMMQMKVNYNHNICTQVYHKSISRRCTLPVTAIVSSLHYEGKMRTTNEY
                     NKPIVVDTTGSTKPDPGDLVLTCFRGWVKQLQIDYRGHEVMTAAASQGLTRKGVYAVRQ
                     KVNENPLYASTSEHVNVLLTRTEGKLVWKTLSGDPWIKTLQNPPKGNFKATIKEWEVEH
                     ASIMAGICNHQVTFDTFQNKANVCWAKSLVPILETAGIKLNDRQWSQIIQAFKEDRAYS
                     PEVALNEICTRMYGVDLDSGLFSKPLVSVHHADNHWDNRPGGKMFGFNPEAASILERKY
                     PFTKGKWNTNKQICVTTRRIEDFNPNTNIIPANRRLPHSLVAEHRPVKGERMEWLVNKI
                     NGHHVLLVSGYNLVLPTKRVTWVAPLGIRGADYTYNLELGLPATLGRYDLVIINIHTPF
                     RIHHYQQCVDHAMKLQMLGGDSLRLLKPGGSLLIRAYGYADRTSERVVCVLGRKFRSSR
                     ALKPPCVTSNTEMFFLFSNFDNGRRNFTTHVMNNQLNAAFVGQATRAGCAPSYRVKRMD
                     IAKNDEECVVNAANPRGLPGDGVCKAVYKKWPESFKNSATPVGTAKTVMCGTYPVIHAV
                     GPNFSNYSESEGDRELAAAYREVAKEVTRLGVNSVAIPLLSTGVYSGGKDRLTQSLNHL
                     FTALDSTDADVVIYCRDKEWEKKIAEAIQMRTQVELLDEHISVDCDIIRVHPDSSLAGR
                     KGYSTTEGSLYSYLEGTRFHQTAVDMAEVYTMWPKQTEANEQVCLYALGESIESIRQKC
                     PVDDADASSPPKTVPCLCRYAMTPERVTRLRMNHVTSIIVCSSFPLPKYKIEGVQKVKC
                     SKVMLFDHNVPSRVSPREYKSPQETAQEVSSTTSLTHSQFDLSVDGEELPAPSDLEADA
                     PIPEPTPDDRAVLTLPPTIDNFSAVSDWVMNTAPVAPPRRRRGKNLNVTCDEREGNVLP
                     MASVRFFRADLHSIVQETAEIRDTAASLQAPLSVATEPNQLPISFGAPNETFPITFGDF
                     DEGEIESLSSELLTFGDFSPGEVDDLTDSDWSTCSDTDDEL*LDRAGGYIFSSDTGPGH
                     LQQRSVRQTVLPVNTLEEVQEEKCYPPKLDEVKEQLLLKKLQESASMANRSRYQSRKVE
                     NMKATIVQRLKGGCKLYLMSETPKVPTYRTTYPAPVYSPPINIRLSNPESAVAACNEFL
                     ARNYPTVASYQITDEYDAYLDMVDGSESCLDRATFNPSKLRSYPKQHSYHAPTIRSAVP
                     SPFQNTLQNVLAAATKRNCNVTQMRELPTLDSAVFNVECFKKFACNQEYWKEFAASPIR
                     ITTENLTTYVTKLKGPKAAALFAKTHNLLPLQEVPMDRFTVDMKRDVKVTPGTKHTEER
                     PKVQVIQAAEPLATAYLCGIHRELVRRLNAVLLPNVHTLFDMSAEDFDAIIAAHFKPGD
                     AVLETDIASFDKSQDDSLALTALMLLEDLGVDHPLLDLIEAAFGEISSCHLPTGTRFKF
                     GAMMKSGMFLTLFVNTLLNITIASRVLEDRLTRSACAAFIGDDNIIHGVVSDELMAARC
                     ATWMNMEVKIIDAVVSQKAPYFCGGFILYDTVAGTACRVADPLKRLFKLGKPLAAGDEQ
                     DDDRRRALADEVVRWQRTGLTDELEKAVHSRYEVQGISVVVMSMATFASSRSNFEKLRG
                     PVVTLYGGPK"
     RBS             4355..4363
                     /label="Shine-Dalgarno sequence"
                     /note="full consensus sequence for ribosome-binding sites
                     upstream of start codons in E. coli; complementary to a
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     regulatory      7480..7499
                     /label="first subgenomic promoter"
                     /note="first subgenomic promoter"
                     /regulatory_class="promoter"
     CDS             7576..8292
                     /label="EGFP"
                     /note="enhanced GFP"
     regulatory      8367..8386
                     /label="second subgenomic promoter"
                     /note="second subgenomic promoter"
                     /regulatory_class="promoter"
     CDS             8456..12199
                     /note="Structural polyprotein from Chikungunya virus
                     (strain 37997). Accession#: Q5XXP3"
                     /label="Structural polyprotein"
     3'UTR           12203..12768
     polyA_site      12769
     promoter        12884..12988
                     /label="AmpR promoter"
     CDS             12989..13846
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      14020..14608
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     promoter        14696..14713
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"