LOCUS       V008524                14713 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008524
VERSION     V008524
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 14713)
  AUTHORS   Tsetsarkin KA, Vanlandingham DL, Higgs S.
  TITLE     GFP-expressing full length clone of Chikungunya virus strain 37997
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14713)
  AUTHORS   Tsetsarkin KA, Vanlandingham DL, Higgs S.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-OCT-2007) Pathology, University of Texas Medical
            Branch, University Blvd, Galveston, TX 77555-0609, USA
REFERENCE   3  (bases 1 to 14713)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 14713)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (17-OCT-2007) Pathology, University of Texas Medical Branch,
            University Blvd, Galveston, TX 77555-0609, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..14713
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     5'UTR           1..77
     CDS             78..7502
                     /codon_start=1
                     /transl_except=(pos: 5646 .. 5648,  aa: OTHER)
                     /product="nonstructural polyprotein"
                     /label="nonstructural polyprotein"
                     /note="encodes nonstructural proteins 1 through 4"
                     /protein_id="ABX40012.1"
                     /translation="MDPVYVDIDADSAFLKALQRAYPMFEVEPRQVTPNDHANARAFSH
                     LAIKLIEQEIDPDSTILDIGSAPARRMMSDRKYHCVCPMRSAEDPERLANYARKLASAA
                     GKVLDRNISEKIGDLQAVMAVPDAETPTFCLHTDVSCRQRADVAIYQDVYAVHAPTSLY
                     HQAIKGVRVAYWIGFDTTPFMYNAMAGAYPSYSTNWADEQVLKAKNIGLCSTDLTEGRR
                     GKLSIMRGKKMKPCDRVLFSVGSTLYPESRKLLKSWHLPSVFHLKGKLSFTCRCDTVVS
                     CEGYVVKRITISPGLYGKTTGYAVTHHADGFLMCKTTDTVDGERVSFSVCTYVPATICD
                     QMTGILATEVTPEDAQKLLVGLNQRIVVNGRTQRNTNTMKNYLLPVVAQALSKWAKECR
                     KDMEDEKLLGIRERTLTCCCLWAFKKQKTHTVYKRPDTQSIQKVPAEFDSFVVPSLWSS
                     GLSIPLRTRIKWLLSKVPKTDLIPYSGDAKEARDAEKEAEEEREAELTREALPPLQAAQ
                     DDVQVEIDVEQLEDRAGAGIIETPRGAIKVTAQPTDHVVGEYLVLSPQTVLRSQKLSLI
                     HALAEQVKTCTHSGRAGRYAVEAYDGRILVPSGYAISPEDFQSLSESATMVYNEREFVN
                     RKLHHIALHGPALNTDEESYELVRAERTEHEYVYDVDQRRCCKKEEAAGLVLVGDLTNP
                     PYHEFAYEGLRIRPACPYKTAVIGVFGVPGSGKSAIIKNLVTRQDLVTSGKKENCQEIS
                     TDVMRQRNLEISARTVDSLLLNGCNRPVDVLYVDEAFACHSGTLLALIALVRPRQKVVL
                     CGDPKQCGFFNMMQMKVNYNHNICTQVYHKSISRRCTLPVTAIVSSLHYEGKMRTTNEY
                     NKPIVVDTTGSTKPDPGDLVLTCFRGWVKQLQIDYRGHEVMTAAASQGLTRKGVYAVRQ
                     KVNENPLYASTSEHVNVLLTRTEGKLVWKTLSGDPWIKTLQNPPKGNFKATIKEWEVEH
                     ASIMAGICNHQVTFDTFQNKANVCWAKSLVPILETAGIKLNDRQWSQIIQAFKEDRAYS
                     PEVALNEICTRMYGVDLDSGLFSKPLVSVHHADNHWDNRPGGKMFGFNPEAASILERKY
                     PFTKGKWNTNKQICVTTRRIEDFNPNTNIIPANRRLPHSLVAEHRPVKGERMEWLVNKI
                     NGHHVLLVSGYNLVLPTKRVTWVAPLGIRGADYTYNLELGLPATLGRYDLVIINIHTPF
                     RIHHYQQCVDHAMKLQMLGGDSLRLLKPGGSLLIRAYGYADRTSERVVCVLGRKFRSSR
                     ALKPPCVTSNTEMFFLFSNFDNGRRNFTTHVMNNQLNAAFVGQATRAGCAPSYRVKRMD
                     IAKNDEECVVNAANPRGLPGDGVCKAVYKKWPESFKNSATPVGTAKTVMCGTYPVIHAV
                     GPNFSNYSESEGDRELAAAYREVAKEVTRLGVNSVAIPLLSTGVYSGGKDRLTQSLNHL
                     FTALDSTDADVVIYCRDKEWEKKIAEAIQMRTQVELLDEHISVDCDIIRVHPDSSLAGR
                     KGYSTTEGSLYSYLEGTRFHQTAVDMAEVYTMWPKQTEANEQVCLYALGESIESIRQKC
                     PVDDADASSPPKTVPCLCRYAMTPERVTRLRMNHVTSIIVCSSFPLPKYKIEGVQKVKC
                     SKVMLFDHNVPSRVSPREYKSPQETAQEVSSTTSLTHSQFDLSVDGEELPAPSDLEADA
                     PIPEPTPDDRAVLTLPPTIDNFSAVSDWVMNTAPVAPPRRRRGKNLNVTCDEREGNVLP
                     MASVRFFRADLHSIVQETAEIRDTAASLQAPLSVATEPNQLPISFGAPNETFPITFGDF
                     DEGEIESLSSELLTFGDFSPGEVDDLTDSDWSTCSDTDDEL*LDRAGGYIFSSDTGPGH
                     LQQRSVRQTVLPVNTLEEVQEEKCYPPKLDEVKEQLLLKKLQESASMANRSRYQSRKVE
                     NMKATIVQRLKGGCKLYLMSETPKVPTYRTTYPAPVYSPPINIRLSNPESAVAACNEFL
                     ARNYPTVASYQITDEYDAYLDMVDGSESCLDRATFNPSKLRSYPKQHSYHAPTIRSAVP
                     SPFQNTLQNVLAAATKRNCNVTQMRELPTLDSAVFNVECFKKFACNQEYWKEFAASPIR
                     ITTENLTTYVTKLKGPKAAALFAKTHNLLPLQEVPMDRFTVDMKRDVKVTPGTKHTEER
                     PKVQVIQAAEPLATAYLCGIHRELVRRLNAVLLPNVHTLFDMSAEDFDAIIAAHFKPGD
                     AVLETDIASFDKSQDDSLALTALMLLEDLGVDHPLLDLIEAAFGEISSCHLPTGTRFKF
                     GAMMKSGMFLTLFVNTLLNITIASRVLEDRLTRSACAAFIGDDNIIHGVVSDELMAARC
                     ATWMNMEVKIIDAVVSQKAPYFCGGFILYDTVAGTACRVADPLKRLFKLGKPLAAGDEQ
                     DDDRRRALADEVVRWQRTGLTDELEKAVHSRYEVQGISVVVMSMATFASSRSNFEKLRG
                     PVVTLYGGPK"
     RBS             4355..4363
                     /label="Shine-Dalgarno sequence"
                     /note="full consensus sequence for ribosome-binding sites
                     upstream of start codons in E. coli; complementary to a
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     regulatory      7480..7499
                     /label="first subgenomic promoter"
                     /note="first subgenomic promoter"
                     /regulatory_class="promoter"
     CDS             7576..8292
                     /label="EGFP"
                     /note="enhanced GFP"
     regulatory      8367..8386
                     /label="second subgenomic promoter"
                     /note="second subgenomic promoter"
                     /regulatory_class="promoter"
     CDS             8456..12199
                     /note="Structural polyprotein from Chikungunya virus
                     (strain 37997). Accession#: Q5XXP3"
                     /label="Structural polyprotein"
     3'UTR           12203..12768
     polyA_site      12769
     promoter        12884..12988
                     /label="AmpR promoter"
     CDS             12989..13846
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      14020..14608
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     promoter        14696..14713
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 atggctgcgt gagacacacg tagcctacca gtttcttact gctctactct gcttagcaag
       61 agacttgaga acccatcatg gatcccgtgt acgtggacat agacgccgac agcgcctttt
      121 taaaggccct gcagcgtgcg taccccatgt ttgaggtgga accaaggcag gtcacaccga
      181 atgaccatgc caatgctaga gcattctcgc atctagctat aaaactaata gagcaggaaa
      241 ttgatcccga ctcaaccatc ctggacatag gcagcgcgcc agcaaggagg atgatgtcgg
      301 ataggaagta ccactgcgtt tgccctatgc gcagcgcaga agaccctgag agactcgcca
      361 actacgcgag aaaactagca tctgccgcag gaaaagtctt ggacagaaac atctccgaaa
      421 aaattggaga tctacaagca gtaatggctg taccagacgc agaaacgccc acattctgct
      481 tgcacactga cgtctcatgt agacaaaggg cggacgtcgc tatataccag gatgtctacg
      541 ccgtgcatgc accaacatcg ctgtaccacc aggcgattaa aggagtccgt gtagcatact
      601 ggatagggtt tgatacaacc ccgttcatgt ataatgccat ggcaggtgca tacccctcgt
      661 actcgacaaa ctgggcagat gagcaggtgc tgaaggcaaa gaacatagga ttatgttcaa
      721 cagacctgac ggaaggtaga cgaggtaaat tgtctatcat gagaggaaaa aagatgaagc
      781 catgtgaccg cgtactgttc tcagtcgggt caacgcttta cccggagagc cgtaagcttc
      841 ttaagagttg gcacttacct tcagtgttcc atctaaaagg gaagctcagc ttcacgtgcc
      901 gctgtgatac agtggtttcg tgtgaaggct atgtcgttaa gagaataacg attagcccgg
      961 gcctctacgg taaaaccaca gggtacgcag taacccacca tgcagacgga ttcctaatgt
     1021 gcaaaacaac cgatacggta gatggcgaga gagtgtcatt ttcggtatgc acgtacgtac
     1081 ccgcaaccat ttgtgatcaa atgacaggta ttcttgccac ggaggttaca ccggaggatg
     1141 cacagaagct gctggtggga ctgaaccaga ggatagtggt caatggcaga acgcagagga
     1201 acacgaacac aatgaagaat tacttgcttc ctgtagttgc ccaagccctc agtaagtggg
     1261 caaaggaatg ccggaaagat atggaagatg aaaaactttt gggcatcaga gaaaggacac
     1321 tgacatgctg ctgcctttgg gcgttcaaga agcagaagac acacacggtc tacaagaggc
     1381 ctgacactca gtcaattcag aaagtcccag ccgaatttga cagctttgtg gtaccaagtc
     1441 tgtggtcatc tggactgtcg atcccgctac ggaccagaat caagtggctg ctaagcaaag
     1501 tgccaaagac tgatttgatc ccttacagcg gtgacgccaa agaagcccgc gacgctgaaa
     1561 aagaagcaga agaagaacga gaagcggagc taactcgcga ggcactacca ccactacagg
     1621 cggcacagga cgacgtccag gtcgaaattg acgtggaaca gctcgaagac agagctgggg
     1681 caggaataat tgaaactcca agaggagcta tcaaagtcac tgcccaacca acagaccacg
     1741 tcgtgggaga gtacttggta ctttccccgc agaccgtgtt acgaagccag aagctcagcc
     1801 tgatccacgc attggcggaa caagtgaaga catgcacaca cagcggacgg gcaggaaggt
     1861 acgcggtcga agcatatgac ggcagaatcc ttgtgccctc aggctatgca atatcacctg
     1921 aagacttcca gagcctgagc gaaagtgcga cgatggtgta caacgaaagg gagttcgtaa
     1981 ataggaaatt acaccatatc gcgttgcacg gaccagccct gaacactgac gaggagtcgt
     2041 acgagctggt aagggcagaa aggacagagc atgagtacgt ctatgatgtg gaccaaagaa
     2101 ggtgctgcaa gaaagaggag gcagccgggc tggtactggt cggcgacttg accaacccgc
     2161 cctaccatga gttcgcatat gaagggctga gaatccgccc cgcctgccca tacaagaccg
     2221 cagtaatagg ggtctttgga gtgccaggat ccggcaaatc agcaatcatt aagaacctag
     2281 ttaccaggca agacctagtg accagtggaa agaaagaaaa ctgccaagaa atctccaccg
     2341 acgtgatgcg acagaggaac ctggagatat ctgcacgcac ggtcgactca ctgctcttga
     2401 acggatgcaa tagaccagtc gacgtgttgt acgtcgacga agcttttgcg tgccattctg
     2461 gcacgctact tgctctgata gccttggtga gaccgaggca gaaagtcgtg ctatgcggtg
     2521 atccgaaaca gtgcggcttc ttcaatatga tgcagatgaa agttaactac aaccataaca
     2581 tctgcaccca agtgtaccat aaaagtattt ccaggcggtg tacactgcct gtgactgcca
     2641 ttgtgtcctc gttgcattac gaaggcaaaa tgcgcacaac aaatgagtac aacaagccaa
     2701 ttgtagtgga tactacaggc tcgacaaaac ccgaccccgg agaccttgtg ctaacatgtt
     2761 tcagagggtg ggttaagcaa ctgcaaattg actatcgtgg acacgaggtc atgacagcag
     2821 ctgcatctca ggggctaacc agaaaagggg tctatgccgt caggcaaaaa gttaatgaaa
     2881 acccccttta cgcatcaaca tcagagcacg tgaacgtgct actgacgcgt acggaaggca
     2941 aactagtatg gaagacactt tctggagacc catggataaa gacactgcag aacccgccga
     3001 aaggaaattt taaagcaaca attaaggaat gggaagtgga acatgcttca ataatggcgg
     3061 gtatctgtaa ccaccaagtg acctttgaca cgttccagaa taaagccaat gtctgctggg
     3121 cgaagagctt agtccccatc ctagaaacag cagggataaa attaaacgac aggcagtggt
     3181 cccagataat ccaggctttt aaagaagaca gagcatactc acccgaggtg gccctgaatg
     3241 agatatgcac gcgcatgtac ggggtagacc tggacagcgg actgttctct aaaccactgg
     3301 tgtccgtgca tcatgcggat aatcactggg acaacaggcc gggagggaag atgttcggat
     3361 tcaaccccga agcggcgtcc atactggaga ggaaataccc gtttacaaaa gggaagtgga
     3421 ataccaacaa gcaaatctgt gtgactacta ggaggattga agattttaac ccgaacacca
     3481 acattatacc tgccaacagg agattaccgc attcattggt ggccgaacat cgcccggtaa
     3541 aaggggagag gatggaatgg ttggtcaaca aaataaatgg ccaccatgtg ctcctggtca
     3601 gcggctacaa cctcgttctg cccactaaga gagtcacctg ggtggcgccg ctgggcattc
     3661 ggggagctga ctacacatac aacctagagt taggcctacc agcaacgctc ggtagatatg
     3721 acctagtgat tataaacatc cacacaccct ttcgcataca tcattaccaa cagtgcgtgg
     3781 atcacgcaat gaagctgcag atgctcggag gagactccct gagactgctc aagccgggtg
     3841 gttcattact gatcagggca tacggctacg cagacagaac aagcgaacga gtagtctgcg
     3901 tattgggacg caagtttcga tcatccagag cgttgaaacc gccgtgcgtc actagcaaca
     3961 ccgagatgtt tttcttgttc agcaactttg ataacggcag aaggaacttt acgacgcacg
     4021 taatgaacaa ccagctgaat gctgcttttg ttggtcaggc cacccgagca gggtgcgcac
     4081 cgtcgtaccg ggttaaacgc atggacatcg caaagaacga tgaagagtgt gtagtcaacg
     4141 ccgccaaccc tcgtgggcta ccaggcgatg gcgtctgtaa agcagtatac aaaaaatggc
     4201 cggagtcctt caagaacagt gcaacaccag tgggaaccgc aaagacagtc atgtgcggta
     4261 catacccggt aatccatgca gtaggaccta atttctcaaa ttactctgag tccgaaggag
     4321 accgggaatt ggcagctgct taccgagaag tcgctaagga ggtgactaga ctaggagtaa
     4381 acagcgtagc tataccgctc ctttccaccg gtgtgtactc tggagggaaa gacaggctga
     4441 ctcagtcact aaaccacctt tttacagcat tagactcaac tgatgcagat gtggttatct
     4501 actgccgcga caaggagtgg gagaagaaaa tagctgaggc catacaaatg aggacccaag
     4561 tggaattact agacgaacac atctctgtag actgcgatat catccgagtg caccctgaca
     4621 gcagtttggc aggtagaaaa gggtacagca ctacagaagg ttcactgtac tcctacttgg
     4681 aagggacacg gttccatcag acggcagtgg acatggcaga agtatacacc atgtggccaa
     4741 agcagacgga ggctaatgaa caagtttgct tgtacgcatt gggggaaagt atagaatcaa
     4801 tcaggcaaaa gtgcccagtg gatgacgcag atgcatcgtc gcccccaaaa accgtcccgt
     4861 gcctctgccg ttatgccatg acacccgaac gagtcaccag gcttcgtatg aaccatgtca
     4921 caagcataat agtatgctca tcattccccc ttccaaagta taaaatagaa ggagtgcaga
     4981 aagtcaagtg ttctaaagtg atgctgttcg accataacgt gccatcacgc gttagtccaa
     5041 gggaatataa atcgcctcag gagaccgcac aagaagtaag ttcgaccacg tcactgacgc
     5101 acagccaatt cgaccttagc gttgacggtg aggaactgcc cgctccgtct gacttggaag
     5161 ctgacgctcc gattccggaa ccaacaccag acgacagagc ggtacttact ttgcctccca
     5221 cgattgataa tttttcggct gtgtcagact gggtaatgaa taccgcgcca gtcgcaccac
     5281 ccagaagaag acgtgggaaa aacttgaatg tcacctgcga cgagagagaa gggaacgtac
     5341 ttcccatggc tagcgttcgg ttcttcagag cggatctgca ctccatcgta caggaaacgg
     5401 cagagatacg cgatacggcc gcgtccctcc aggcgcccct gagtgtcgct acagaaccga
     5461 atcaactgcc gatctcattt ggagcaccaa acgagacttt ccccataacg ttcggggatt
     5521 ttgatgaagg ggagattgaa agcttgtcct ctgagttact gacctttggg gacttctcgc
     5581 cgggcgaagt ggatgacctg acagacagcg actggtccac gtgttcagac acggacgacg
     5641 aattatgact agatagggca ggtgggtaca tattctcatc tgacaccggc cccggccacc
     5701 tgcaacagag gtctgtccgt cagacagtac tgccggtaaa taccttggag gaagttcagg
     5761 aggagaaatg ttacccacct aagttggatg aagtgaaaga gcagttgtta cttaagaaac
     5821 tccaggaaag tgcgtccatg gctaacagaa gcaggtacca atcccgcaaa gtagagaaca
     5881 tgaaagcaac aatagtccaa aggctgaagg gtggttgcaa actttattta atgtcggaga
     5941 ccccgaaagt tcctacctac cgaactacat atccggcacc agtgtactca cccccaatca
     6001 atatccgact gtccaacccc gagtctgctg tggcagcgtg caatgagttc ctagcaagga
     6061 actatccgac agttgcgtcg taccaaatca ccgatgagta cgatgcatac ctagacatgg
     6121 tggacgggtc ggaaagttgc cttgaccggg cgacgttcaa cccatcaaag cttagaagtt
     6181 atccaaaaca gcactcctac catgcaccca caatcagaag tgccgtacct tccccgttcc
     6241 agaacacgct gcagaacgta ctggctgctg ccacgaaaag aaattgcaac gtcacacaga
     6301 tgagagaact gcctactttg gattcagcgg tatttaatgt tgagtgcttt aaaaaatttg
     6361 cgtgcaatca agaatactgg aaggaatttg ccgccagccc tattaggata acgactgaga
     6421 acttgacaac ttatgtcaca aaactaaaag gaccaaaagc agcagcactg tttgccaaga
     6481 cacataacct gctaccactg caggaggtgc cgatggacag gtttactgta gacatgaaaa
     6541 gggacgtgaa ggtgactccg gggacgaagc acactgagga aagacctaaa gtgcaggtca
     6601 tacaggcagc cgaacctttg gcaacagcat atctgtgtgg gatccacaga gagttggtca
     6661 gaaggctgaa tgcagtcctt ctacctaatg tacacacgct gtttgacatg tctgccgagg
     6721 actttgacgc cattattgcc gcgcacttca agccggggga cgccgtattg gaaaccgata
     6781 tagcctcctt tgacaagagc caagacgact cattggcgct cactgctcta atgttgctag
     6841 aggatttggg ggtggatcat cccctgttgg acttgataga ggctgccttc ggggagatct
     6901 ccagctgcca cctaccgacg ggcacccgtt ttaagttcgg cgccatgatg aagtctggta
     6961 tgttcctaac cctgttcgtc aacacactgc taaacatcac catagccagc cgagtgctgg
     7021 aggaccgctt gacaaggtct gcgtgcgcgg ccttcatcgg cgacgacaat ataatacatg
     7081 gggttgtctc tgacgaactg atggcagcaa ggtgtgctac atggatgaac atggaagtga
     7141 agatcataga tgcggtcgtg tctcagaaag ccccgtactt ctgcggaggg tttatactgt
     7201 atgacacagt agcaggcacg gcctgcagag tggcagaccc gctaaagcgg ctgttcaagc
     7261 tgggcaaacc gctggcagcg ggagatgaac aagacgacga cagaagacgt gcactggctg
     7321 acgaagtggt tagatggcaa cgaacaggac taactgatga gctagaaaaa gcggtacact
     7381 ccaggtatga agtgcagggc atatctgtcg tggtaatgtc tatggccacc tttgcaagct
     7441 ctagatctaa ctttgagaag ctcagaggac ccgtcgtaac cctgtacggt ggtcctaaat
     7501 aggtacgcac tacagctacc tatttcgtca gaaaccaatc gcagctactt gcatacctac
     7561 cagctacggc gcgccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
     7621 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
     7681 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
     7741 gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac
     7801 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
     7861 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
     7921 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
     7981 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc
     8041 gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc
     8101 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
     8161 ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag
     8221 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
     8281 gagctgtaca agtaattaat taatgtctat ggccaccttt gcaagctcta gatctaactt
     8341 tgagaagctc agaggacccg tcgtaaccct gtacggtggt cctaaatagg tacgcactac
     8401 agctacctat ttcgtcagaa accaatcgca gctacttgca tacctaccag ctacaatgga
     8461 gttcatcccg acgcaaactt tctataacag aaggtaccaa ccccgaccct gggccccacg
     8521 ccctacaatt caagtaatta gacctagacc acgtccacag aggcaggctg ggcaactcgc
     8581 ccagctgatc tccgcagtca acaaattgac catgcgcgcg gtacctcaac agaagcctcg
     8641 cagaaatcgg aaaaacaaga agcaaaggca gaagaagcag gcgccgcaaa acgacccaaa
     8701 gcaaaagaag caaccaccac aaaagaagcc ggctcaaaag aagaagaaac caggccgtag
     8761 ggagagaatg tgcatgaaaa ttgaaaatga ttgcatcttc gaagtcaagc atgaaggcaa
     8821 agtgatgggc tacgcatgcc tggtggggga taaagtaatg aaaccagcac atgtgaaggg
     8881 aactatcgac aatgccgatc tggctaaact ggcctttaag cggtcgtcta aatacgatct
     8941 tgaatgtgca cagataccgg tgcacatgaa gtctgatgcc tcgaagttta cccacgagaa
     9001 acccgagggg tactataact ggcatcacgg agcagtgcag tattcaggag gccggttcac
     9061 tatcccgacg ggtgcaggca agccgggaga cagcggcaga ccgatcttcg acaacaaagg
     9121 acgggtggtg gccatcgtcc taggaggggc caacgaaggt gcccgcacgg ccctctccgt
     9181 ggtgacgtgg aacaaagaca tcgtcacaaa aattacccct gagggagccg aagagtggag
     9241 cctcgccctc ccggtcttgt gcctgttggc aaacactaca ttcccctgct ctcagccgcc
     9301 ttgcacaccc tgctgctacg aaaaggaacc ggaaagcacc ttgcgcatgc ttgaggacaa
     9361 cgtgatgaga cccggatact accagctact aaaagcatcg ctgacttgct ctccccaccg
     9421 ccaaagacgc agtactaagg acaattttaa tgtctataaa gccacaagac catatctagc
     9481 tcattgtcct gactgcggag aagggcattc gtgccacagc cctatcgcat tggagcgcat
     9541 cagaaatgaa gcaacggacg gaacgctgaa aatccaggtc tctttgcaga tcgggataaa
     9601 gacagatgac agccacgatt ggaccaagct gcgctatatg gatagccata cgccagcgga
     9661 cgcggagcga gccggattgc ttgtaaggac ttcagcaccg tgcacgatca ccgggaccat
     9721 gggacacttt attctcgccc gatgcccgaa aggagagacg ctgacagtgg gatttacgga
     9781 cagcagaaag atcagccaca catgcacaca cccgttccat catgaaccac ctgtgatagg
     9841 tagggagagg ttccactctc gaccacaaca tggtaaagag ttaccttgca gcacgtacgt
     9901 gcagagcacc gctgccactg ctgaggagat agaggtgcat atgcccccag atactcctga
     9961 ccgcacgctg atgacgcagc agtctggcaa cgtgaagatc acagttaatg ggcagacggt
    10021 gcggtacaag tgcaactgcg gtggctcaaa cgagggactg acaaccacag acaaagtgat
    10081 caataactgc aaaattgatc agtgccatgc tgcagtcact aatcacaaga attggcaata
    10141 caactcccct ttagtcccgc gcaacgctga actcggggac cgtaaaggaa agatccacat
    10201 cccattccca ttggcaaacg tgacttgcag agtgccaaaa gcaagaaacc ctacagtaac
    10261 ttacggaaaa aaccaagtca ccatgctgct gtatcctgac catccgacac tcttgtctta
    10321 ccgtaacatg ggacaggaac caaattacca cgaggagtgg gtgacacaca agaaggaggt
    10381 taccttgacc gtgcctactg agggtctgga ggtcacttgg ggcaacaacg aaccatacaa
    10441 gtactggccg cagatgtcta cgaacggtac tgctcatggt cacccacatg agataatctt
    10501 gtactattat gagctgtacc ccactatgac tgtagtcatt gtgtcggtgg cctcgttcgt
    10561 gcttctgtcg atggtgggca cagcagtggg aatgtgtgtg tgcgcacggc gcagatgcat
    10621 tacaccatat gaattaacac caggagccac tgttcccttc ctgctcagcc tgctatgctg
    10681 cgtcagaacg accaaggcgg ccacatatta cgaggctgcg gcatatctat ggaacgaaca
    10741 gcagcccctg ttctggttgc aggctcttat cccgctggcc gccttgatcg tcctgtgcaa
    10801 ctgtctgaaa ctcttgccat gctgctgtaa gaccctggct tttttagccg taatgagcat
    10861 cggtgcccac actgtgagcg cgtacgaaca cgtaacagtg atcccgaaca cggtgggagt
    10921 accgtataag actcttgtca acagaccggg ttacagcccc atggtgttgg agatggagct
    10981 acaatcagtc accttggaac caacactgtc acttgactac atcacgtgcg agtacaaaac
    11041 tgtcatcccc tccccgtacg tgaagtgctg tggtacagca gagtgcaagg acaagagcct
    11101 accagactac agctgcaagg tctttactgg agtctaccca tttatgtggg gcggcgccta
    11161 ctgcttttgc gacgccgaaa atacgcaatt gagcgaggca catgtagaga aatctgaatc
    11221 ttgcaaaaca gagtttgcat cggcctacag agcccacacc gcatcggcgt cggcgaagct
    11281 ccgcgtcctt taccaaggaa acaacattac cgtagctgcc tacgctaacg gtgaccatgc
    11341 cgtcacagta aaggacgcca agtttgtcgt gggcccaatg tcctccgcct ggacaccttt
    11401 tgacaacaaa atcgtggtgt acaaaggcga cgtctacaac atggactacc caccttttgg
    11461 cgcaggaaga ccaggacaat ttggtgacat tcaaagtcgt acaccggaaa gtaaagacgt
    11521 ttatgccaac actcagttgg tactacagag gccagcagca ggcacggtac atgtaccata
    11581 ctctcaggca ccatctggct tcaagtattg gctgaaggaa cgaggagcat cgctacagca
    11641 cacggcaccg ttcggttgcc agattgcgac aaacccggta agagctgtaa attgcgctgt
    11701 ggggaacata ccaatttcca tcgacatacc ggatgcggcc tttactaggg ttgtcgatgc
    11761 accctctgta acggacatgt catgcgaagt accagcctgc actcactcct ccgactttgg
    11821 gggcgtcgcc atcatcaaat acacagctag caagaaaggt aaatgtgcag tacattcgat
    11881 gaccaacgcc gttaccattc gagaagccga cgtagaagta gaggggaact cccagctgca
    11941 aatatccttc tcaacagccc tggcaagcgc cgagtttcgc gtgcaagtgt gctccacaca
    12001 agtacactgc gcagccgcat gccaccctcc aaaggaccac atagtcaatt acccagcatc
    12061 acacaccacc cttggggtcc aggatatatc cacaacggca atgtcttggg tgcagaagat
    12121 tacgggagga gtaggattaa ttgttgctgt tgctgcctta attttaattg tggtgctatg
    12181 cgtgtcgttt agcaggcact aaaccgatga taaggcacga aataactaaa tagcaaaagt
    12241 agaaagtaca taaccaggta tatgtgcccc ttaagaggca caatatatat agctaagcac
    12301 tattagatca aagggctata caacccctga atagtaacaa aacacaaaaa ccaataaaaa
    12361 tcataaaaag aaaaatctca taaacaggta taagtgtccc ctaagagaca cattgtatgt
    12421 aggtagtaag tatagatcaa agggctatat taacccctga atagtaacaa aacacaaaaa
    12481 caataaaaac tacaaaatag aaaatctata aacaaaagta gttcaaaggg ctacaaaacc
    12541 cctgaatagt aacaaaacat aaaatgtaat aaaaattaag tgtgtaccca aaagaggtac
    12601 agtaagaatc agtgaatatc acaattggca acgagaagag acgtaggtat ttaagcttcc
    12661 taaaagcagc cgaactcact ttgagacgta ggcatagcat accgaactct tccactattc
    12721 tccgaaccca cagggacgta ggagatgtta ttttgttttt aatatttcaa aaaaaaaaaa
    12781 aaaaaaaaaa aaaaaaaaaa aaaaaaaagc ggccgcttaa ttaatcgagg ggaattaatt
    12841 cttgaagacg aaagggccag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
    12901 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
    12961 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
    13021 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
    13081 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
    13141 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
    13201 agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg
    13261 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
    13321 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
    13381 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
    13441 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
    13501 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
    13561 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
    13621 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
    13681 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
    13741 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
    13801 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
    13861 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
    13921 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
    13981 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
    14041 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
    14101 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
    14161 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
    14221 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
    14281 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
    14341 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
    14401 acagcgtgag cattgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
    14461 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
    14521 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
    14581 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcgagctcgt atggacatat
    14641 tgtcgttaga acgcggctac aattaataca taaccttatg tatcatacac aatcgattta
    14701 ggtgacacta tag
//
