pCHC01 vector (V008529)

LOCUS       40924_10631        8786 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCHC01, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8786)
  AUTHORS   Chen C-H., DeMay BS, Gladfelter AS, Dunlap JC, Loros JJ.
  TITLE     Direct interaction between VVD and WCC regulates photoadaptation in 
            Neurospora
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8786)
  AUTHORS   Chen C-H.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAR-2010) Genetics, Dartmouth Medical School, 702 
            Remsen, Hanover, NH 03755, USA
REFERENCE   3  (bases 1 to 8786)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8786)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (18-MAR-2010) Genetics, Dartmouth Medical School, 702 Remsen, 
            Hanover, NH 03755, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8786
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        196..416
                     /label=URA3 promoter
     CDS             417..1217
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     rep_origin      complement(1351..1806)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     misc_recomb     1957..3025
                     /note="derived from Neurospora crassa csr-1; Neurospora 
                     homologous recombination site"
     misc_recomb     3026..3054
                     /label=yeast homologous recombination site
                     /note="yeast homologous recombination site"
     promoter        3055..3072
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_recomb     3085..3113
                     /label=yeast homologous recombination site
                     /note="yeast homologous recombination site"
     CDS             complement(3264..3812)
                     /label=BlpR
                     /note="phosphinothricin acetyltransferase"
     promoter        complement(3835..4194)
                     /label=trpC promoter
                     /note="promoter for Aspergillus nidulans trpC"
     misc_recomb     4206..5220
                     /note="derived from Neurospora crassa csr-1; Neurospora 
                     homologous recombination site"
     protein_bind    complement(5247..5263)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5271..5301)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5316..5337)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5625..6213)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6387..7244)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(7245..7349)
                     /label=AmpR promoter
     rep_origin      complement(7376..8718)
                     /direction=LEFT
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"